Experiment: 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF30 and GFF31 are separated by 197 nucleotides GFF31 and GFF32 are separated by 52 nucleotides GFF32 and GFF33 are separated by 83 nucleotides GFF33 and GFF34 are separated by 62 nucleotides GFF34 and GFF35 are separated by 55 nucleotides
GFF30 - YaeQ protein, at 7,852 to 8,397
GFF30
GFF31 - FIG00639395: hypothetical protein, at 8,595 to 8,795
GFF31
GFF32 - Rho-specific inhibitor of transcription termination (YaeO), at 8,848 to 9,042
GFF32
GFF33 - tRNA(Ile)-lysidine synthetase (EC 6.3.4.19), at 9,126 to 10,418
GFF33
GFF34 - FIG01280259: hypothetical protein, at 10,481 to 10,870
GFF34
GFF35 - Lysine decarboxylase 2, constitutive (EC 4.1.1.18), at 10,926 to 13,067
GFF35
Position (kb)
9
10
11 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 8.250 kb on - strand, within GFF30 at 8.373 kb on + strand at 8.445 kb on + strand at 8.511 kb on + strand at 8.512 kb on - strand at 8.512 kb on - strand at 8.513 kb on + strand at 8.513 kb on + strand at 8.513 kb on + strand at 8.513 kb on + strand at 8.513 kb on + strand at 8.514 kb on - strand at 8.514 kb on - strand at 8.526 kb on - strand at 8.551 kb on - strand at 8.604 kb on + strand at 8.617 kb on + strand, within GFF31 at 8.653 kb on + strand, within GFF31 at 8.658 kb on + strand, within GFF31 at 8.696 kb on + strand, within GFF31 at 8.781 kb on + strand at 8.800 kb on + strand at 8.825 kb on - strand at 8.829 kb on + strand at 8.830 kb on - strand at 8.859 kb on - strand at 8.938 kb on + strand, within GFF32 at 10.430 kb on - strand at 10.432 kb on - strand at 10.454 kb on - strand at 10.454 kb on - strand at 10.482 kb on + strand at 10.483 kb on - strand at 10.576 kb on + strand, within GFF34 at 10.710 kb on + strand, within GFF34 at 10.722 kb on - strand, within GFF34 at 10.860 kb on - strand at 11.348 kb on - strand, within GFF35
Per-strain Table
Position Strand Gene LocusTag Fraction 6F2_phage 15 MOI remove 8,250 - GFF30 0.73 -0.7 8,373 + +0.8 8,445 + +0.4 8,511 + -0.9 8,512 - -0.8 8,512 - -2.5 8,513 + -1.0 8,513 + +0.3 8,513 + +1.0 8,513 + -0.1 8,513 + -0.1 8,514 - -0.4 8,514 - +0.0 8,526 - +0.0 8,551 - -0.3 8,604 + -1.7 8,617 + GFF31 0.11 -1.7 8,653 + GFF31 0.29 -0.1 8,658 + GFF31 0.31 -1.2 8,696 + GFF31 0.50 -0.3 8,781 + +0.6 8,800 + -0.2 8,825 - -0.4 8,829 + -1.6 8,830 - -2.0 8,859 - +0.2 8,938 + GFF32 0.46 -0.5 10,430 - +2.3 10,432 - +0.0 10,454 - +0.5 10,454 - -0.7 10,482 + -0.5 10,483 - +0.0 10,576 + GFF34 0.24 +0.1 10,710 + GFF34 0.59 -2.3 10,722 - GFF34 0.62 +1.5 10,860 - +0.8 11,348 - GFF35 0.20 -2.8
Or see this region's nucleotide sequence