Experiment: 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF312 and GFF313 overlap by 8 nucleotides GFF313 and GFF314 are separated by 62 nucleotides GFF314 and GFF315 are separated by 9 nucleotides
GFF312 - Putative carbohydrate PTS system, IIC component (EC 2.7.1.69), at 29,490 to 30,848
GFF312
GFF313 - Putative carbohydrate kinase in cluster with fructose-bisphosphate aldolase, at 30,841 to 32,325
GFF313
GFF314 - Fructose-bisphosphate aldolase (EC 4.1.2.13), at 32,388 to 33,248
GFF314
GFF315 - Phosphotransferase system HPr enzyme STM3779, at 33,258 to 33,527
GFF315
Position (kb)
30
31
32
33 Strain fitness (log2 ratio)
-2
-1
0
1 at 29.849 kb on + strand, within GFF312 at 29.858 kb on + strand, within GFF312 at 29.859 kb on - strand, within GFF312 at 29.859 kb on - strand, within GFF312 at 29.883 kb on + strand, within GFF312 at 30.019 kb on - strand, within GFF312 at 30.195 kb on - strand, within GFF312 at 30.228 kb on + strand, within GFF312 at 30.342 kb on - strand, within GFF312 at 30.363 kb on + strand, within GFF312 at 30.434 kb on + strand, within GFF312 at 30.434 kb on + strand, within GFF312 at 30.455 kb on + strand, within GFF312 at 30.455 kb on + strand, within GFF312 at 30.456 kb on - strand, within GFF312 at 30.492 kb on - strand, within GFF312 at 30.767 kb on + strand at 30.768 kb on - strand at 30.850 kb on + strand at 30.885 kb on + strand at 31.066 kb on - strand, within GFF313 at 31.145 kb on - strand, within GFF313 at 31.204 kb on + strand, within GFF313 at 31.204 kb on + strand, within GFF313 at 31.204 kb on + strand, within GFF313 at 31.205 kb on - strand, within GFF313 at 31.211 kb on + strand, within GFF313 at 31.238 kb on - strand, within GFF313 at 31.273 kb on + strand, within GFF313 at 31.273 kb on + strand, within GFF313 at 31.273 kb on + strand, within GFF313 at 31.307 kb on + strand, within GFF313 at 31.308 kb on - strand, within GFF313 at 31.375 kb on - strand, within GFF313 at 31.466 kb on + strand, within GFF313 at 31.535 kb on + strand, within GFF313 at 31.564 kb on - strand, within GFF313 at 31.638 kb on - strand, within GFF313 at 31.648 kb on - strand, within GFF313 at 32.406 kb on - strand at 32.799 kb on - strand, within GFF314 at 32.917 kb on + strand, within GFF314 at 32.917 kb on + strand, within GFF314 at 32.917 kb on + strand, within GFF314 at 32.959 kb on + strand, within GFF314 at 33.044 kb on + strand, within GFF314 at 33.055 kb on + strand, within GFF314 at 33.056 kb on - strand, within GFF314 at 33.255 kb on + strand at 33.281 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 6F2_phage 15 MOI remove 29,849 + GFF312 0.26 +0.1 29,858 + GFF312 0.27 -0.0 29,859 - GFF312 0.27 -0.4 29,859 - GFF312 0.27 +0.0 29,883 + GFF312 0.29 -1.3 30,019 - GFF312 0.39 +0.0 30,195 - GFF312 0.52 -0.1 30,228 + GFF312 0.54 -0.6 30,342 - GFF312 0.63 +0.0 30,363 + GFF312 0.64 +0.2 30,434 + GFF312 0.69 -2.1 30,434 + GFF312 0.69 -0.0 30,455 + GFF312 0.71 -1.1 30,455 + GFF312 0.71 -1.1 30,456 - GFF312 0.71 -0.4 30,492 - GFF312 0.74 +0.4 30,767 + -0.4 30,768 - -1.1 30,850 + -1.2 30,885 + -0.6 31,066 - GFF313 0.15 -0.2 31,145 - GFF313 0.20 +0.2 31,204 + GFF313 0.24 +0.5 31,204 + GFF313 0.24 +0.4 31,204 + GFF313 0.24 -0.2 31,205 - GFF313 0.25 -0.2 31,211 + GFF313 0.25 -0.4 31,238 - GFF313 0.27 -0.0 31,273 + GFF313 0.29 +0.2 31,273 + GFF313 0.29 +0.1 31,273 + GFF313 0.29 +0.0 31,307 + GFF313 0.31 -2.3 31,308 - GFF313 0.31 +0.2 31,375 - GFF313 0.36 +0.1 31,466 + GFF313 0.42 -0.4 31,535 + GFF313 0.47 +0.1 31,564 - GFF313 0.49 -0.8 31,638 - GFF313 0.54 -0.1 31,648 - GFF313 0.54 -0.3 32,406 - +0.3 32,799 - GFF314 0.48 +0.1 32,917 + GFF314 0.61 -0.0 32,917 + GFF314 0.61 -0.2 32,917 + GFF314 0.61 +0.1 32,959 + GFF314 0.66 +0.3 33,044 + GFF314 0.76 +0.1 33,055 + GFF314 0.77 +0.5 33,056 - GFF314 0.78 +0.3 33,255 + -0.6 33,281 + -0.3
Or see this region's nucleotide sequence