Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF24

Experiment: 6F2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF23 and GFF24 are separated by 391 nucleotidesGFF24 and GFF25 overlap by 37 nucleotidesGFF25 and GFF26 are separated by 110 nucleotides GFF23 - Methionine ABC transporter substrate-binding protein, at 2,751 to 3,566 GFF23 GFF24 - Protein RcsF, at 3,958 to 4,089 GFF24 GFF25 - COG1720: Uncharacterized conserved protein, at 4,053 to 4,793 GFF25 GFF26 - Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type, at 4,904 to 6,622 GFF26 Position (kb) 3 4 5Strain fitness (log2 ratio) -2 -1 0 1at 3.363 kb on + strand, within GFF23at 3.364 kb on - strand, within GFF23at 3.592 kb on - strandat 3.631 kb on - strandat 3.651 kb on - strandat 3.681 kb on + strandat 3.740 kb on + strandat 3.839 kb on - strandat 3.957 kb on + strandat 3.957 kb on + strandat 3.958 kb on - strandat 4.209 kb on + strand, within GFF25at 4.405 kb on + strand, within GFF25at 4.490 kb on + strand, within GFF25at 4.604 kb on + strand, within GFF25at 4.643 kb on + strand, within GFF25at 4.686 kb on - strand, within GFF25

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Per-strain Table

Position Strand Gene LocusTag Fraction 6F2_phage 15 MOI
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3,363 + GFF23 0.75 -2.1
3,364 - GFF23 0.75 +0.7
3,592 - +0.2
3,631 - -1.3
3,651 - +0.2
3,681 + -0.6
3,740 + -2.0
3,839 - -0.1
3,957 + -1.7
3,957 + -1.8
3,958 - +0.2
4,209 + GFF25 0.21 -0.6
4,405 + GFF25 0.48 -0.4
4,490 + GFF25 0.59 -0.6
4,604 + GFF25 0.74 +0.5
4,643 + GFF25 0.80 -0.0
4,686 - GFF25 0.85 +0.1

Or see this region's nucleotide sequence