Experiment: 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1995 and GFF1996 are separated by 322 nucleotides GFF1996 and GFF1997 overlap by 8 nucleotides GFF1997 and GFF1998 are separated by 10 nucleotides
GFF1995 - Translation elongation factor P Lys34:lysine transferase, at 9,402 to 10,379
GFF1995
GFF1996 - Succinate dehydrogenase flavoprotein subunit (EC 1.3.99.1), at 10,702 to 12,492
GFF1996
GFF1997 - Succinate dehydrogenase iron-sulfur protein (EC 1.3.99.1), at 12,485 to 13,219
GFF1997
GFF1998 - Fumarate reductase subunit C, at 13,230 to 13,625
GFF1998
Position (kb)
10
11
12
13 Strain fitness (log2 ratio)
-2
-1
0
1 at 10.011 kb on - strand, within GFF1995 at 10.416 kb on + strand at 10.504 kb on - strand at 10.594 kb on + strand at 10.595 kb on - strand at 10.595 kb on - strand at 10.608 kb on + strand at 10.680 kb on + strand at 10.681 kb on - strand at 10.685 kb on + strand at 10.686 kb on - strand at 10.733 kb on + strand at 10.733 kb on + strand at 10.733 kb on + strand at 10.895 kb on - strand, within GFF1996 at 11.106 kb on + strand, within GFF1996 at 11.115 kb on + strand, within GFF1996 at 11.116 kb on - strand, within GFF1996 at 11.182 kb on - strand, within GFF1996 at 11.305 kb on + strand, within GFF1996 at 11.472 kb on + strand, within GFF1996 at 11.548 kb on - strand, within GFF1996 at 11.716 kb on + strand, within GFF1996 at 11.770 kb on + strand, within GFF1996 at 11.771 kb on - strand, within GFF1996 at 11.771 kb on - strand, within GFF1996 at 12.100 kb on + strand, within GFF1996 at 12.100 kb on + strand, within GFF1996 at 12.136 kb on - strand, within GFF1996 at 12.170 kb on + strand, within GFF1996 at 12.171 kb on - strand, within GFF1996 at 12.214 kb on + strand, within GFF1996 at 12.214 kb on + strand, within GFF1996 at 12.215 kb on - strand, within GFF1996 at 12.217 kb on - strand, within GFF1996 at 12.255 kb on + strand, within GFF1996 at 12.255 kb on + strand, within GFF1996 at 12.469 kb on - strand at 12.611 kb on + strand, within GFF1997 at 12.611 kb on + strand, within GFF1997 at 12.738 kb on - strand, within GFF1997 at 12.842 kb on + strand, within GFF1997 at 12.842 kb on + strand, within GFF1997 at 12.847 kb on + strand, within GFF1997 at 12.847 kb on + strand, within GFF1997 at 12.951 kb on - strand, within GFF1997 at 13.238 kb on + strand at 13.251 kb on + strand at 13.251 kb on + strand at 13.252 kb on - strand at 13.252 kb on - strand at 13.305 kb on + strand, within GFF1998 at 13.306 kb on - strand, within GFF1998 at 13.307 kb on + strand, within GFF1998 at 13.308 kb on - strand, within GFF1998
Per-strain Table
Position Strand Gene LocusTag Fraction 6F2_phage 15 MOI remove 10,011 - GFF1995 0.62 -0.5 10,416 + -1.3 10,504 - +0.1 10,594 + -1.1 10,595 - -0.4 10,595 - -0.5 10,608 + -0.3 10,680 + -0.1 10,681 - +0.4 10,685 + -0.6 10,686 - -1.3 10,733 + +0.9 10,733 + -1.0 10,733 + +0.5 10,895 - GFF1996 0.11 +0.6 11,106 + GFF1996 0.23 +0.9 11,115 + GFF1996 0.23 -0.6 11,116 - GFF1996 0.23 +0.4 11,182 - GFF1996 0.27 +0.1 11,305 + GFF1996 0.34 -0.0 11,472 + GFF1996 0.43 +0.9 11,548 - GFF1996 0.47 -1.9 11,716 + GFF1996 0.57 -2.6 11,770 + GFF1996 0.60 -0.1 11,771 - GFF1996 0.60 -0.8 11,771 - GFF1996 0.60 -0.3 12,100 + GFF1996 0.78 -0.8 12,100 + GFF1996 0.78 +0.1 12,136 - GFF1996 0.80 +0.4 12,170 + GFF1996 0.82 +0.4 12,171 - GFF1996 0.82 -0.3 12,214 + GFF1996 0.84 +0.4 12,214 + GFF1996 0.84 +0.0 12,215 - GFF1996 0.84 +1.6 12,217 - GFF1996 0.85 +0.7 12,255 + GFF1996 0.87 -0.2 12,255 + GFF1996 0.87 +1.1 12,469 - -0.6 12,611 + GFF1997 0.17 -0.9 12,611 + GFF1997 0.17 -0.6 12,738 - GFF1997 0.34 -0.2 12,842 + GFF1997 0.49 +0.4 12,842 + GFF1997 0.49 +0.3 12,847 + GFF1997 0.49 +0.5 12,847 + GFF1997 0.49 -1.1 12,951 - GFF1997 0.63 +1.4 13,238 + -0.4 13,251 + +0.2 13,251 + -1.1 13,252 - -0.5 13,252 - +0.3 13,305 + GFF1998 0.19 +0.2 13,306 - GFF1998 0.19 -0.1 13,307 + GFF1998 0.19 +0.3 13,308 - GFF1998 0.20 +0.5
Or see this region's nucleotide sequence