Experiment: 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1455 and GFF1456 overlap by 4 nucleotides GFF1456 and GFF1457 overlap by 8 nucleotides GFF1457 and GFF1458 overlap by 4 nucleotides GFF1458 and GFF1459 are separated by 139 nucleotides GFF1459 and GFF1460 are separated by 76 nucleotides
GFF1455 - NrfC protein, at 118,346 to 119,017
GFF1455
GFF1456 - NrfD protein, at 119,014 to 119,970
GFF1456
GFF1457 - Cytochrome c heme lyase subunit CcmF, at 119,963 to 122,185
GFF1457
GFF1458 - Cytochrome c-type heme lyase subunit nrfG, nitrite reductase complex assembly, at 122,182 to 122,802
GFF1458
GFF1459 - hypothetical protein, at 122,942 to 123,070
GFF1459
GFF1460 - Glutamate-aspartate carrier protein, at 123,147 to 124,457
GFF1460
Position (kb)
119
120
121
122
123 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 118.994 kb on - strand at 118.997 kb on + strand at 118.998 kb on - strand at 119.075 kb on - strand at 119.164 kb on - strand, within GFF1456 at 119.164 kb on - strand, within GFF1456 at 119.275 kb on + strand, within GFF1456 at 119.323 kb on + strand, within GFF1456 at 119.330 kb on + strand, within GFF1456 at 119.330 kb on + strand, within GFF1456 at 119.331 kb on - strand, within GFF1456 at 119.466 kb on - strand, within GFF1456 at 119.497 kb on + strand, within GFF1456 at 119.545 kb on - strand, within GFF1456 at 119.631 kb on + strand, within GFF1456 at 119.632 kb on - strand, within GFF1456 at 119.632 kb on - strand, within GFF1456 at 119.663 kb on + strand, within GFF1456 at 119.687 kb on + strand, within GFF1456 at 119.687 kb on + strand, within GFF1456 at 119.688 kb on - strand, within GFF1456 at 119.688 kb on - strand, within GFF1456 at 119.866 kb on - strand, within GFF1456 at 120.155 kb on - strand at 120.377 kb on - strand, within GFF1457 at 120.397 kb on + strand, within GFF1457 at 120.398 kb on - strand, within GFF1457 at 120.493 kb on - strand, within GFF1457 at 120.669 kb on - strand, within GFF1457 at 120.767 kb on + strand, within GFF1457 at 120.767 kb on + strand, within GFF1457 at 120.768 kb on - strand, within GFF1457 at 120.925 kb on + strand, within GFF1457 at 120.966 kb on - strand, within GFF1457 at 121.104 kb on - strand, within GFF1457 at 121.104 kb on - strand, within GFF1457 at 121.189 kb on - strand, within GFF1457 at 121.269 kb on + strand, within GFF1457 at 121.270 kb on - strand, within GFF1457 at 121.270 kb on - strand, within GFF1457 at 121.270 kb on - strand, within GFF1457 at 121.364 kb on + strand, within GFF1457 at 121.365 kb on - strand, within GFF1457 at 121.410 kb on - strand, within GFF1457 at 121.410 kb on - strand, within GFF1457 at 121.410 kb on - strand, within GFF1457 at 121.410 kb on - strand, within GFF1457 at 121.539 kb on + strand, within GFF1457 at 121.599 kb on + strand, within GFF1457 at 121.601 kb on + strand, within GFF1457 at 121.601 kb on + strand, within GFF1457 at 121.601 kb on + strand, within GFF1457 at 121.602 kb on - strand, within GFF1457 at 121.602 kb on - strand, within GFF1457 at 121.804 kb on - strand, within GFF1457 at 122.003 kb on - strand at 122.229 kb on - strand at 122.452 kb on + strand, within GFF1458 at 122.452 kb on + strand, within GFF1458 at 122.452 kb on + strand, within GFF1458 at 122.577 kb on + strand, within GFF1458 at 122.577 kb on + strand, within GFF1458 at 122.577 kb on + strand, within GFF1458 at 122.578 kb on - strand, within GFF1458 at 122.578 kb on - strand, within GFF1458 at 122.578 kb on - strand, within GFF1458 at 122.607 kb on + strand, within GFF1458 at 122.607 kb on + strand, within GFF1458 at 122.607 kb on + strand, within GFF1458 at 122.608 kb on - strand, within GFF1458 at 123.028 kb on - strand, within GFF1459 at 123.035 kb on - strand, within GFF1459 at 123.088 kb on + strand at 123.089 kb on - strand at 123.092 kb on + strand at 123.093 kb on - strand at 123.093 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 6F2_phage 15 MOI remove 118,994 - -0.2 118,997 + -0.3 118,998 - +0.8 119,075 - -0.2 119,164 - GFF1456 0.16 +0.3 119,164 - GFF1456 0.16 +0.3 119,275 + GFF1456 0.27 -0.8 119,323 + GFF1456 0.32 -0.3 119,330 + GFF1456 0.33 +0.7 119,330 + GFF1456 0.33 -1.9 119,331 - GFF1456 0.33 -0.3 119,466 - GFF1456 0.47 +0.4 119,497 + GFF1456 0.50 -0.4 119,545 - GFF1456 0.55 +0.6 119,631 + GFF1456 0.64 +0.3 119,632 - GFF1456 0.65 -0.5 119,632 - GFF1456 0.65 -1.2 119,663 + GFF1456 0.68 +1.7 119,687 + GFF1456 0.70 -1.7 119,687 + GFF1456 0.70 -0.4 119,688 - GFF1456 0.70 +0.7 119,688 - GFF1456 0.70 -0.3 119,866 - GFF1456 0.89 +0.3 120,155 - +0.4 120,377 - GFF1457 0.19 +0.1 120,397 + GFF1457 0.20 -0.2 120,398 - GFF1457 0.20 +0.5 120,493 - GFF1457 0.24 +0.6 120,669 - GFF1457 0.32 +1.2 120,767 + GFF1457 0.36 -2.0 120,767 + GFF1457 0.36 -1.0 120,768 - GFF1457 0.36 -0.1 120,925 + GFF1457 0.43 +0.3 120,966 - GFF1457 0.45 -0.2 121,104 - GFF1457 0.51 -0.2 121,104 - GFF1457 0.51 +0.8 121,189 - GFF1457 0.55 +0.4 121,269 + GFF1457 0.59 -0.8 121,270 - GFF1457 0.59 -0.5 121,270 - GFF1457 0.59 -0.1 121,270 - GFF1457 0.59 +0.5 121,364 + GFF1457 0.63 +2.1 121,365 - GFF1457 0.63 +0.2 121,410 - GFF1457 0.65 -0.4 121,410 - GFF1457 0.65 +1.4 121,410 - GFF1457 0.65 +0.2 121,410 - GFF1457 0.65 -0.3 121,539 + GFF1457 0.71 +1.5 121,599 + GFF1457 0.74 +0.3 121,601 + GFF1457 0.74 +0.1 121,601 + GFF1457 0.74 -1.8 121,601 + GFF1457 0.74 -1.0 121,602 - GFF1457 0.74 -0.8 121,602 - GFF1457 0.74 -0.1 121,804 - GFF1457 0.83 +0.9 122,003 - +0.5 122,229 - -0.9 122,452 + GFF1458 0.43 -1.3 122,452 + GFF1458 0.43 -0.2 122,452 + GFF1458 0.43 -0.0 122,577 + GFF1458 0.64 +0.4 122,577 + GFF1458 0.64 +0.2 122,577 + GFF1458 0.64 +0.6 122,578 - GFF1458 0.64 -0.4 122,578 - GFF1458 0.64 -0.1 122,578 - GFF1458 0.64 -0.2 122,607 + GFF1458 0.68 -0.1 122,607 + GFF1458 0.68 +0.1 122,607 + GFF1458 0.68 -0.7 122,608 - GFF1458 0.69 -0.8 123,028 - GFF1459 0.67 +0.5 123,035 - GFF1459 0.72 -0.4 123,088 + +1.6 123,089 - +0.2 123,092 + +0.9 123,093 - +0.8 123,093 - -0.3
Or see this region's nucleotide sequence