Experiment: 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1280 and GFF1281 are separated by 12 nucleotides GFF1281 and GFF1282 are separated by 54 nucleotides GFF1282 and GFF1283 are separated by 57 nucleotides GFF1283 and GFF1284 are separated by 10 nucleotides
GFF1280 - Phage capsid and scaffold, at 7,381 to 8,877
GFF1280
GFF1281 - Head decoration protein, at 8,890 to 9,237
GFF1281
GFF1282 - Phage major capsid protein, at 9,292 to 10,320
GFF1282
GFF1283 - Phage capsid and scaffold, at 10,378 to 10,737
GFF1283
GFF1284 - Phage capsid and scaffold, at 10,748 to 11,131
GFF1284
Position (kb)
9
10
11 Strain fitness (log2 ratio)
-2
-1
0
1 at 8.431 kb on - strand, within GFF1280 at 8.478 kb on + strand, within GFF1280 at 8.479 kb on - strand, within GFF1280 at 8.683 kb on - strand, within GFF1280 at 8.823 kb on + strand at 8.853 kb on + strand at 8.889 kb on + strand at 8.889 kb on + strand at 8.890 kb on - strand at 8.890 kb on - strand at 9.025 kb on - strand, within GFF1281 at 9.122 kb on - strand, within GFF1281 at 9.291 kb on + strand at 9.448 kb on + strand, within GFF1282 at 9.544 kb on - strand, within GFF1282 at 9.548 kb on - strand, within GFF1282 at 9.709 kb on + strand, within GFF1282 at 9.771 kb on + strand, within GFF1282 at 9.778 kb on - strand, within GFF1282 at 9.793 kb on - strand, within GFF1282 at 9.846 kb on + strand, within GFF1282 at 9.850 kb on + strand, within GFF1282 at 9.879 kb on + strand, within GFF1282 at 10.147 kb on + strand, within GFF1282 at 10.147 kb on + strand, within GFF1282 at 10.148 kb on - strand, within GFF1282 at 10.247 kb on - strand at 10.247 kb on - strand at 10.248 kb on + strand at 10.248 kb on + strand at 10.249 kb on - strand at 10.400 kb on + strand at 10.401 kb on - strand at 10.610 kb on + strand, within GFF1283 at 10.611 kb on - strand, within GFF1283 at 10.660 kb on - strand, within GFF1283 at 10.747 kb on + strand at 10.865 kb on - strand, within GFF1284 at 10.989 kb on + strand, within GFF1284 at 11.050 kb on + strand, within GFF1284 at 11.051 kb on - strand, within GFF1284 at 11.053 kb on + strand, within GFF1284 at 11.054 kb on - strand, within GFF1284 at 11.308 kb on + strand at 11.317 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 6F2_phage 15 MOI remove 8,431 - GFF1280 0.70 -0.3 8,478 + GFF1280 0.73 +0.8 8,479 - GFF1280 0.73 +0.2 8,683 - GFF1280 0.87 -0.0 8,823 + -0.4 8,853 + +0.8 8,889 + +0.2 8,889 + -0.3 8,890 - -0.3 8,890 - +0.8 9,025 - GFF1281 0.39 -0.0 9,122 - GFF1281 0.67 -0.4 9,291 + +0.0 9,448 + GFF1282 0.15 -0.0 9,544 - GFF1282 0.24 +0.0 9,548 - GFF1282 0.25 +0.2 9,709 + GFF1282 0.41 +0.2 9,771 + GFF1282 0.47 -0.6 9,778 - GFF1282 0.47 +1.1 9,793 - GFF1282 0.49 +0.7 9,846 + GFF1282 0.54 +1.4 9,850 + GFF1282 0.54 -2.7 9,879 + GFF1282 0.57 -0.2 10,147 + GFF1282 0.83 +0.0 10,147 + GFF1282 0.83 +1.0 10,148 - GFF1282 0.83 +0.6 10,247 - -0.3 10,247 - +0.3 10,248 + -0.3 10,248 + -0.2 10,249 - +0.7 10,400 + +1.3 10,401 - -0.2 10,610 + GFF1283 0.64 -0.2 10,611 - GFF1283 0.65 -0.4 10,660 - GFF1283 0.78 +0.0 10,747 + -0.5 10,865 - GFF1284 0.30 -0.3 10,989 + GFF1284 0.63 -0.2 11,050 + GFF1284 0.79 -2.0 11,051 - GFF1284 0.79 +0.1 11,053 + GFF1284 0.79 +0.1 11,054 - GFF1284 0.80 +0.1 11,308 + -0.3 11,317 - +0.5
Or see this region's nucleotide sequence