Experiment: Br60_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4176 and GFF4177 are separated by 9 nucleotides GFF4177 and GFF4178 are separated by 57 nucleotides GFF4178 and GFF4179 overlap by 40 nucleotides
GFF4176 - 2-keto-3-deoxy-D-arabino-heptulosonate-7- phosphate synthase I alpha (EC 2.5.1.54), at 64,952 to 66,022
GFF4176
GFF4177 - Chorismate mutase I (EC 5.4.99.5) / Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43), at 66,032 to 67,153
GFF4177
GFF4178 - FIG074102: hypothetical protein, at 67,211 to 68,119
GFF4178
GFF4179 - Chorismate mutase I (EC 5.4.99.5) / Prephenate dehydratase (EC 4.2.1.51), at 68,080 to 69,240
GFF4179
Position (kb)
66
67
68 Strain fitness (log2 ratio)
-1
0
1 at 65.062 kb on - strand, within GFF4176 at 65.124 kb on + strand, within GFF4176 at 65.124 kb on + strand, within GFF4176 at 65.168 kb on + strand, within GFF4176 at 65.169 kb on - strand, within GFF4176 at 65.216 kb on + strand, within GFF4176 at 65.676 kb on - strand, within GFF4176 at 65.676 kb on - strand, within GFF4176 at 65.728 kb on - strand, within GFF4176 at 65.745 kb on + strand, within GFF4176 at 65.745 kb on + strand, within GFF4176 at 65.821 kb on + strand, within GFF4176 at 65.863 kb on + strand, within GFF4176 at 65.863 kb on + strand, within GFF4176 at 65.863 kb on + strand, within GFF4176 at 65.944 kb on + strand at 65.945 kb on - strand at 65.971 kb on - strand at 66.066 kb on + strand at 66.187 kb on + strand, within GFF4177 at 66.188 kb on - strand, within GFF4177 at 66.188 kb on - strand, within GFF4177 at 66.278 kb on + strand, within GFF4177 at 66.539 kb on - strand, within GFF4177 at 66.938 kb on + strand, within GFF4177 at 66.939 kb on - strand, within GFF4177 at 66.939 kb on - strand, within GFF4177 at 66.949 kb on + strand, within GFF4177 at 66.950 kb on - strand, within GFF4177 at 66.952 kb on + strand, within GFF4177 at 66.992 kb on + strand, within GFF4177 at 66.992 kb on + strand, within GFF4177 at 66.992 kb on + strand, within GFF4177 at 67.165 kb on - strand at 67.167 kb on - strand at 67.167 kb on - strand at 67.170 kb on - strand at 67.216 kb on + strand at 67.230 kb on + strand at 67.292 kb on + strand at 67.351 kb on + strand, within GFF4178 at 67.352 kb on - strand, within GFF4178 at 67.432 kb on + strand, within GFF4178 at 67.432 kb on + strand, within GFF4178 at 67.447 kb on + strand, within GFF4178 at 67.458 kb on + strand, within GFF4178 at 67.492 kb on + strand, within GFF4178 at 67.574 kb on + strand, within GFF4178 at 67.575 kb on - strand, within GFF4178 at 67.686 kb on - strand, within GFF4178 at 67.686 kb on - strand, within GFF4178 at 67.686 kb on - strand, within GFF4178 at 67.687 kb on + strand, within GFF4178 at 67.713 kb on + strand, within GFF4178 at 67.715 kb on + strand, within GFF4178 at 67.715 kb on + strand, within GFF4178 at 67.716 kb on - strand, within GFF4178 at 67.716 kb on - strand, within GFF4178 at 67.826 kb on + strand, within GFF4178 at 67.960 kb on + strand, within GFF4178 at 67.969 kb on + strand, within GFF4178 at 67.969 kb on + strand, within GFF4178
Per-strain Table
Position Strand Gene LocusTag Fraction Br60_phage 15 MOI remove 65,062 - GFF4176 0.10 -0.1 65,124 + GFF4176 0.16 -0.6 65,124 + GFF4176 0.16 +0.6 65,168 + GFF4176 0.20 +1.1 65,169 - GFF4176 0.20 -0.5 65,216 + GFF4176 0.25 -1.4 65,676 - GFF4176 0.68 +1.6 65,676 - GFF4176 0.68 -0.4 65,728 - GFF4176 0.72 +0.5 65,745 + GFF4176 0.74 -0.5 65,745 + GFF4176 0.74 -0.2 65,821 + GFF4176 0.81 +0.3 65,863 + GFF4176 0.85 -0.5 65,863 + GFF4176 0.85 +0.1 65,863 + GFF4176 0.85 +0.3 65,944 + +0.8 65,945 - +0.6 65,971 - +1.3 66,066 + +1.2 66,187 + GFF4177 0.14 -0.1 66,188 - GFF4177 0.14 -0.2 66,188 - GFF4177 0.14 -1.3 66,278 + GFF4177 0.22 +0.2 66,539 - GFF4177 0.45 -0.3 66,938 + GFF4177 0.81 +0.8 66,939 - GFF4177 0.81 +1.0 66,939 - GFF4177 0.81 +0.5 66,949 + GFF4177 0.82 +0.2 66,950 - GFF4177 0.82 +1.1 66,952 + GFF4177 0.82 -0.3 66,992 + GFF4177 0.86 +0.2 66,992 + GFF4177 0.86 -0.0 66,992 + GFF4177 0.86 -0.2 67,165 - -1.1 67,167 - +0.0 67,167 - +0.8 67,170 - +0.4 67,216 + -0.4 67,230 + +0.3 67,292 + +0.8 67,351 + GFF4178 0.15 +0.6 67,352 - GFF4178 0.16 +0.4 67,432 + GFF4178 0.24 +0.2 67,432 + GFF4178 0.24 +0.2 67,447 + GFF4178 0.26 -0.1 67,458 + GFF4178 0.27 -0.5 67,492 + GFF4178 0.31 +0.2 67,574 + GFF4178 0.40 +0.6 67,575 - GFF4178 0.40 -0.1 67,686 - GFF4178 0.52 -0.4 67,686 - GFF4178 0.52 -0.1 67,686 - GFF4178 0.52 -0.2 67,687 + GFF4178 0.52 +1.4 67,713 + GFF4178 0.55 +0.8 67,715 + GFF4178 0.55 -0.2 67,715 + GFF4178 0.55 -1.0 67,716 - GFF4178 0.56 +0.1 67,716 - GFF4178 0.56 -0.6 67,826 + GFF4178 0.68 +0.0 67,960 + GFF4178 0.82 +0.1 67,969 + GFF4178 0.83 +0.1 67,969 + GFF4178 0.83 -0.4
Or see this region's nucleotide sequence