Experiment: Br60_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3276 and GFF3277 are separated by 217 nucleotides GFF3277 and GFF3278 are separated by 274 nucleotides GFF3278 and GFF3279 overlap by 4 nucleotides
GFF3276 - 4-hydroxyphenylacetate 3-monooxygenase (EC 1.14.13.3), at 47,592 to 49,154
GFF3276
GFF3277 - Homoprotocatechuate degradative operon repressor, at 49,372 to 49,812
GFF3277
GFF3278 - 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68) / 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC 5.3.3.-), at 50,087 to 51,376
GFF3278
GFF3279 - 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (EC 1.2.1.60), at 51,373 to 52,839
GFF3279
Position (kb)
50
51
52 Strain fitness (log2 ratio)
-1
0
1 at 49.235 kb on + strand at 49.370 kb on + strand at 49.371 kb on - strand at 49.371 kb on - strand at 49.374 kb on - strand at 49.374 kb on - strand at 49.416 kb on + strand, within GFF3277 at 49.517 kb on + strand, within GFF3277 at 49.517 kb on + strand, within GFF3277 at 49.550 kb on + strand, within GFF3277 at 49.550 kb on + strand, within GFF3277 at 49.553 kb on - strand, within GFF3277 at 49.553 kb on - strand, within GFF3277 at 49.553 kb on - strand, within GFF3277 at 49.553 kb on - strand, within GFF3277 at 49.557 kb on + strand, within GFF3277 at 49.557 kb on + strand, within GFF3277 at 50.082 kb on + strand at 50.086 kb on + strand at 50.340 kb on - strand, within GFF3278 at 50.566 kb on - strand, within GFF3278 at 50.780 kb on + strand, within GFF3278 at 50.810 kb on - strand, within GFF3278 at 50.832 kb on + strand, within GFF3278 at 50.901 kb on - strand, within GFF3278 at 50.901 kb on - strand, within GFF3278 at 50.901 kb on - strand, within GFF3278 at 50.909 kb on + strand, within GFF3278 at 50.978 kb on + strand, within GFF3278 at 50.979 kb on - strand, within GFF3278 at 51.085 kb on - strand, within GFF3278 at 51.397 kb on - strand at 51.740 kb on - strand, within GFF3279 at 51.768 kb on + strand, within GFF3279 at 52.066 kb on - strand, within GFF3279 at 52.244 kb on - strand, within GFF3279 at 52.368 kb on + strand, within GFF3279 at 52.369 kb on - strand, within GFF3279
Per-strain Table
Position Strand Gene LocusTag Fraction Br60_phage 15 MOI remove 49,235 + -0.7 49,370 + +0.3 49,371 - +0.2 49,371 - +0.4 49,374 - +0.3 49,374 - -1.1 49,416 + GFF3277 0.10 -0.6 49,517 + GFF3277 0.33 +0.3 49,517 + GFF3277 0.33 -1.0 49,550 + GFF3277 0.40 +0.2 49,550 + GFF3277 0.40 +0.4 49,553 - GFF3277 0.41 +0.2 49,553 - GFF3277 0.41 +0.3 49,553 - GFF3277 0.41 +0.3 49,553 - GFF3277 0.41 +0.0 49,557 + GFF3277 0.42 +0.4 49,557 + GFF3277 0.42 -0.6 50,082 + +0.1 50,086 + +0.5 50,340 - GFF3278 0.20 -1.7 50,566 - GFF3278 0.37 -0.2 50,780 + GFF3278 0.54 +0.6 50,810 - GFF3278 0.56 -1.0 50,832 + GFF3278 0.58 +0.1 50,901 - GFF3278 0.63 +0.2 50,901 - GFF3278 0.63 -0.4 50,901 - GFF3278 0.63 -0.3 50,909 + GFF3278 0.64 -0.5 50,978 + GFF3278 0.69 -0.1 50,979 - GFF3278 0.69 +0.0 51,085 - GFF3278 0.77 -0.7 51,397 - -0.6 51,740 - GFF3279 0.25 -0.3 51,768 + GFF3279 0.27 +0.2 52,066 - GFF3279 0.47 -0.8 52,244 - GFF3279 0.59 +0.7 52,368 + GFF3279 0.68 +0.1 52,369 - GFF3279 0.68 -0.0
Or see this region's nucleotide sequence