Experiment: Br60_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3254 and GFF3255 are separated by 94 nucleotides GFF3255 and GFF3256 are separated by 170 nucleotides GFF3256 and GFF3257 are separated by 44 nucleotides GFF3257 and GFF3258 are separated by 57 nucleotides
GFF3254 - Methylglyoxal synthase (EC 4.2.3.3), at 30,390 to 30,848
GFF3254
GFF3255 - UPF0319 protein YccT precursor, at 30,943 to 31,605
GFF3255
GFF3256 - Succinyl-CoA synthetase, alpha subunit-related enzymes, at 31,776 to 32,192
GFF3256
GFF3257 - hemimethylated DNA binding protein YccV, at 32,237 to 32,554
GFF3257
GFF3258 - LSU m5C1962 methyltransferase RlmI, at 32,612 to 33,823
GFF3258
Position (kb)
31
32
33 Strain fitness (log2 ratio)
-1
0
1 at 30.866 kb on - strand at 31.155 kb on - strand, within GFF3255 at 31.200 kb on + strand, within GFF3255 at 31.528 kb on - strand, within GFF3255 at 31.605 kb on + strand at 31.658 kb on + strand at 31.677 kb on + strand at 31.677 kb on + strand at 31.679 kb on + strand at 31.679 kb on + strand at 31.685 kb on + strand at 31.685 kb on + strand at 31.686 kb on - strand at 31.706 kb on + strand at 31.706 kb on + strand at 31.732 kb on + strand at 31.823 kb on + strand, within GFF3256 at 31.835 kb on - strand, within GFF3256 at 32.049 kb on - strand, within GFF3256 at 32.356 kb on - strand, within GFF3257 at 32.356 kb on - strand, within GFF3257 at 32.359 kb on + strand, within GFF3257 at 32.359 kb on + strand, within GFF3257 at 32.359 kb on + strand, within GFF3257 at 32.359 kb on + strand, within GFF3257 at 32.499 kb on + strand, within GFF3257 at 32.573 kb on - strand at 32.573 kb on - strand at 32.585 kb on + strand at 32.613 kb on + strand at 32.705 kb on - strand at 32.729 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Br60_phage 15 MOI remove 30,866 - -1.5 31,155 - GFF3255 0.32 +0.7 31,200 + GFF3255 0.39 +0.1 31,528 - GFF3255 0.88 +0.4 31,605 + -0.2 31,658 + +0.6 31,677 + -0.7 31,677 + +0.1 31,679 + +0.2 31,679 + +0.3 31,685 + -0.9 31,685 + +0.4 31,686 - -0.3 31,706 + -0.0 31,706 + -0.1 31,732 + -0.2 31,823 + GFF3256 0.11 +0.3 31,835 - GFF3256 0.14 +0.5 32,049 - GFF3256 0.65 +1.2 32,356 - GFF3257 0.37 +0.4 32,356 - GFF3257 0.37 +0.3 32,359 + GFF3257 0.38 -1.2 32,359 + GFF3257 0.38 -0.1 32,359 + GFF3257 0.38 -0.0 32,359 + GFF3257 0.38 -1.0 32,499 + GFF3257 0.82 +0.1 32,573 - -0.1 32,573 - -0.1 32,585 + +0.1 32,613 + +1.0 32,705 - -1.3 32,729 + +0.1
Or see this region's nucleotide sequence