Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3256

Experiment: Br60_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3254 and GFF3255 are separated by 94 nucleotidesGFF3255 and GFF3256 are separated by 170 nucleotidesGFF3256 and GFF3257 are separated by 44 nucleotidesGFF3257 and GFF3258 are separated by 57 nucleotides GFF3254 - Methylglyoxal synthase (EC 4.2.3.3), at 30,390 to 30,848 GFF3254 GFF3255 - UPF0319 protein YccT precursor, at 30,943 to 31,605 GFF3255 GFF3256 - Succinyl-CoA synthetase, alpha subunit-related enzymes, at 31,776 to 32,192 GFF3256 GFF3257 - hemimethylated DNA binding protein YccV, at 32,237 to 32,554 GFF3257 GFF3258 - LSU m5C1962 methyltransferase RlmI, at 32,612 to 33,823 GFF3258 Position (kb) 31 32 33Strain fitness (log2 ratio) -1 0 1at 30.866 kb on - strandat 31.155 kb on - strand, within GFF3255at 31.200 kb on + strand, within GFF3255at 31.528 kb on - strand, within GFF3255at 31.605 kb on + strandat 31.658 kb on + strandat 31.677 kb on + strandat 31.677 kb on + strandat 31.679 kb on + strandat 31.679 kb on + strandat 31.685 kb on + strandat 31.685 kb on + strandat 31.686 kb on - strandat 31.706 kb on + strandat 31.706 kb on + strandat 31.732 kb on + strandat 31.823 kb on + strand, within GFF3256at 31.835 kb on - strand, within GFF3256at 32.049 kb on - strand, within GFF3256at 32.356 kb on - strand, within GFF3257at 32.356 kb on - strand, within GFF3257at 32.359 kb on + strand, within GFF3257at 32.359 kb on + strand, within GFF3257at 32.359 kb on + strand, within GFF3257at 32.359 kb on + strand, within GFF3257at 32.499 kb on + strand, within GFF3257at 32.573 kb on - strandat 32.573 kb on - strandat 32.585 kb on + strandat 32.613 kb on + strandat 32.705 kb on - strandat 32.729 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Br60_phage 15 MOI
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30,866 - -1.5
31,155 - GFF3255 0.32 +0.7
31,200 + GFF3255 0.39 +0.1
31,528 - GFF3255 0.88 +0.4
31,605 + -0.2
31,658 + +0.6
31,677 + -0.7
31,677 + +0.1
31,679 + +0.2
31,679 + +0.3
31,685 + -0.9
31,685 + +0.4
31,686 - -0.3
31,706 + -0.0
31,706 + -0.1
31,732 + -0.2
31,823 + GFF3256 0.11 +0.3
31,835 - GFF3256 0.14 +0.5
32,049 - GFF3256 0.65 +1.2
32,356 - GFF3257 0.37 +0.4
32,356 - GFF3257 0.37 +0.3
32,359 + GFF3257 0.38 -1.2
32,359 + GFF3257 0.38 -0.1
32,359 + GFF3257 0.38 -0.0
32,359 + GFF3257 0.38 -1.0
32,499 + GFF3257 0.82 +0.1
32,573 - -0.1
32,573 - -0.1
32,585 + +0.1
32,613 + +1.0
32,705 - -1.3
32,729 + +0.1

Or see this region's nucleotide sequence