Experiment: Br60_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2006 and GFF2007 are separated by 281 nucleotides GFF2007 and GFF2008 are separated by 47 nucleotides GFF2008 and GFF2009 are separated by 243 nucleotides
GFF2006 - Lysyl-lysine 2,3-aminomutase, at 16,905 to 17,933
GFF2006
GFF2007 - FIG00733146: hypothetical protein, at 18,215 to 19,072
GFF2007
GFF2008 - probable membrane protein yjeI, at 19,120 to 19,473
GFF2008
GFF2009 - Heat shock protein 60 family chaperone GroEL, at 19,717 to 21,363
GFF2009
Position (kb) 18
19
20 Strain fitness (log2 ratio) -1
0
1
2 at 18.136 kb on - strand at 18.266 kb on - strand at 18.381 kb on + strand, within GFF2007 at 18.445 kb on + strand, within GFF2007 at 18.445 kb on + strand, within GFF2007 at 18.758 kb on + strand, within GFF2007 at 18.837 kb on + strand, within GFF2007 at 18.887 kb on - strand, within GFF2007 at 19.036 kb on + strand at 19.041 kb on + strand at 19.041 kb on + strand at 19.076 kb on + strand at 19.119 kb on - strand at 19.119 kb on - strand at 19.135 kb on - strand at 19.201 kb on - strand, within GFF2008 at 19.205 kb on - strand, within GFF2008 at 19.363 kb on - strand, within GFF2008 at 19.526 kb on - strand at 19.541 kb on - strand at 19.593 kb on + strand at 19.593 kb on + strand at 19.661 kb on - strand at 19.718 kb on + strand at 19.719 kb on - strand at 19.744 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Br60_phage 15 MOI remove 18,136 - +0.2 18,266 - -0.3 18,381 + GFF2007 0.19 +0.1 18,445 + GFF2007 0.27 -0.1 18,445 + GFF2007 0.27 +0.3 18,758 + GFF2007 0.63 +0.6 18,837 + GFF2007 0.72 +0.1 18,887 - GFF2007 0.78 +0.3 19,036 + +0.6 19,041 + +0.1 19,041 + -0.3 19,076 + +0.1 19,119 - +0.2 19,119 - -0.0 19,135 - -0.5 19,201 - GFF2008 0.23 +0.8 19,205 - GFF2008 0.24 +0.7 19,363 - GFF2008 0.69 -0.0 19,526 - -0.3 19,541 - -0.1 19,593 + -0.5 19,593 + +0.4 19,661 - -0.9 19,718 + +2.1 19,719 - +0.2 19,744 - +0.2
Or see this region's nucleotide sequence