Experiment: Br60_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1858 and GFF1859 are separated by 2 nucleotides GFF1859 and GFF1860 overlap by 4 nucleotides GFF1860 and GFF1861 overlap by 4 nucleotides GFF1861 and GFF1862 overlap by 4 nucleotides
GFF1858 - UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (EC 2.6.1.-), at 50,346 to 51,503
GFF1858
GFF1859 - Polymyxin resistance protein ArnC, glycosyl transferase (EC 2.4.-.-), at 51,506 to 52,489
GFF1859
GFF1860 - UDP-glucuronic acid oxidase (UDP-4-keto-hexauronic acid decarboxylating) (EC 1.1.1.305) / UDP-4-amino-4-deoxy-L-arabinose formyltransferase (EC 2.1.2.13), at 52,486 to 54,468
GFF1860
GFF1861 - Polymyxin resistance protein PmrJ, predicted deacetylase, at 54,465 to 55,364
GFF1861
GFF1862 - Polymyxin resistance protein ArnT, undecaprenyl phosphate-alpha-L-Ara4N transferase; Melittin resistance protein PqaB, at 55,361 to 57,007
GFF1862
Position (kb) 52
53
54
55 Strain fitness (log2 ratio) -2
-1
0
1
2 at 51.491 kb on - strand at 51.502 kb on - strand at 51.502 kb on - strand at 51.555 kb on - strand at 51.643 kb on - strand, within GFF1859 at 51.938 kb on - strand, within GFF1859 at 52.024 kb on + strand, within GFF1859 at 52.025 kb on - strand, within GFF1859 at 52.140 kb on - strand, within GFF1859 at 52.148 kb on - strand, within GFF1859 at 52.169 kb on + strand, within GFF1859 at 52.169 kb on + strand, within GFF1859 at 52.170 kb on - strand, within GFF1859 at 52.267 kb on + strand, within GFF1859 at 52.268 kb on - strand, within GFF1859 at 52.315 kb on - strand, within GFF1859 at 52.315 kb on - strand, within GFF1859 at 52.319 kb on - strand, within GFF1859 at 52.385 kb on + strand, within GFF1859 at 52.386 kb on - strand, within GFF1859 at 52.400 kb on + strand at 52.401 kb on - strand at 52.671 kb on - strand at 52.671 kb on - strand at 52.732 kb on + strand, within GFF1860 at 52.733 kb on - strand, within GFF1860 at 52.733 kb on - strand, within GFF1860 at 52.980 kb on - strand, within GFF1860 at 53.004 kb on + strand, within GFF1860 at 53.004 kb on + strand, within GFF1860 at 53.005 kb on - strand, within GFF1860 at 53.008 kb on - strand, within GFF1860 at 53.039 kb on + strand, within GFF1860 at 53.040 kb on - strand, within GFF1860 at 53.040 kb on - strand, within GFF1860 at 53.070 kb on + strand, within GFF1860 at 53.071 kb on - strand, within GFF1860 at 53.075 kb on - strand, within GFF1860 at 53.075 kb on - strand, within GFF1860 at 53.075 kb on - strand, within GFF1860 at 53.139 kb on + strand, within GFF1860 at 53.140 kb on - strand, within GFF1860 at 53.185 kb on + strand, within GFF1860 at 53.185 kb on + strand, within GFF1860 at 53.186 kb on - strand, within GFF1860 at 53.186 kb on - strand, within GFF1860 at 53.186 kb on - strand, within GFF1860 at 53.208 kb on + strand, within GFF1860 at 53.340 kb on + strand, within GFF1860 at 53.340 kb on + strand, within GFF1860 at 53.341 kb on - strand, within GFF1860 at 53.464 kb on - strand, within GFF1860 at 53.516 kb on - strand, within GFF1860 at 53.589 kb on + strand, within GFF1860 at 53.757 kb on + strand, within GFF1860 at 53.791 kb on + strand, within GFF1860 at 53.792 kb on - strand, within GFF1860 at 54.174 kb on + strand, within GFF1860 at 54.204 kb on + strand, within GFF1860 at 54.243 kb on + strand, within GFF1860 at 54.304 kb on - strand at 54.307 kb on + strand at 54.308 kb on - strand at 54.497 kb on + strand at 54.649 kb on + strand, within GFF1861 at 54.778 kb on + strand, within GFF1861 at 54.969 kb on + strand, within GFF1861 at 54.970 kb on - strand, within GFF1861 at 54.971 kb on + strand, within GFF1861 at 55.054 kb on + strand, within GFF1861 at 55.153 kb on - strand, within GFF1861 at 55.155 kb on - strand, within GFF1861 at 55.155 kb on - strand, within GFF1861 at 55.155 kb on - strand, within GFF1861 at 55.301 kb on - strand at 55.412 kb on + strand at 55.413 kb on - strand at 55.415 kb on + strand at 55.415 kb on + strand at 55.416 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Br60_phage 15 MOI remove 51,491 - +0.1 51,502 - +0.1 51,502 - +0.2 51,555 - -0.2 51,643 - GFF1859 0.14 -1.1 51,938 - GFF1859 0.44 +0.1 52,024 + GFF1859 0.53 +0.4 52,025 - GFF1859 0.53 +0.1 52,140 - GFF1859 0.64 -0.0 52,148 - GFF1859 0.65 -0.3 52,169 + GFF1859 0.67 +0.9 52,169 + GFF1859 0.67 -0.0 52,170 - GFF1859 0.67 -0.2 52,267 + GFF1859 0.77 +0.1 52,268 - GFF1859 0.77 +0.2 52,315 - GFF1859 0.82 +0.2 52,315 - GFF1859 0.82 +0.0 52,319 - GFF1859 0.83 -0.1 52,385 + GFF1859 0.89 -0.2 52,386 - GFF1859 0.89 +0.4 52,400 + +0.0 52,401 - -0.2 52,671 - -0.1 52,671 - +0.6 52,732 + GFF1860 0.12 +0.4 52,733 - GFF1860 0.12 -0.4 52,733 - GFF1860 0.12 +0.8 52,980 - GFF1860 0.25 -0.2 53,004 + GFF1860 0.26 -0.1 53,004 + GFF1860 0.26 -0.5 53,005 - GFF1860 0.26 +0.2 53,008 - GFF1860 0.26 -0.2 53,039 + GFF1860 0.28 +0.3 53,040 - GFF1860 0.28 -0.2 53,040 - GFF1860 0.28 +0.7 53,070 + GFF1860 0.29 -0.4 53,071 - GFF1860 0.30 -0.4 53,075 - GFF1860 0.30 -0.2 53,075 - GFF1860 0.30 +2.0 53,075 - GFF1860 0.30 -0.0 53,139 + GFF1860 0.33 -0.3 53,140 - GFF1860 0.33 +0.2 53,185 + GFF1860 0.35 -0.5 53,185 + GFF1860 0.35 -0.0 53,186 - GFF1860 0.35 +0.4 53,186 - GFF1860 0.35 -0.3 53,186 - GFF1860 0.35 +1.0 53,208 + GFF1860 0.36 -0.0 53,340 + GFF1860 0.43 +0.4 53,340 + GFF1860 0.43 -0.2 53,341 - GFF1860 0.43 +0.1 53,464 - GFF1860 0.49 +0.4 53,516 - GFF1860 0.52 +1.7 53,589 + GFF1860 0.56 -0.1 53,757 + GFF1860 0.64 +0.7 53,791 + GFF1860 0.66 +0.7 53,792 - GFF1860 0.66 +0.1 54,174 + GFF1860 0.85 +0.0 54,204 + GFF1860 0.87 +0.6 54,243 + GFF1860 0.89 +0.3 54,304 - -0.2 54,307 + +0.1 54,308 - +0.3 54,497 + -1.8 54,649 + GFF1861 0.20 -0.0 54,778 + GFF1861 0.35 -0.4 54,969 + GFF1861 0.56 +0.7 54,970 - GFF1861 0.56 -0.3 54,971 + GFF1861 0.56 -2.1 55,054 + GFF1861 0.65 -0.7 55,153 - GFF1861 0.76 +0.2 55,155 - GFF1861 0.77 -0.2 55,155 - GFF1861 0.77 -2.7 55,155 - GFF1861 0.77 +0.9 55,301 - -0.9 55,412 + +0.9 55,413 - -0.0 55,415 + -0.1 55,415 + -0.3 55,416 - -0.2
Or see this region's nucleotide sequence