Experiment: Plant=Tobacco; PlantTreatment=None; Sample=leaf; GrowthSubstrate=agar; Collection=Direct; Time=3days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5520 and Atu5521 are separated by 26 nucleotides Atu5521 and Atu5522 are separated by 72 nucleotides Atu5522 and Atu5523 overlap by 4 nucleotides
Atu5520: Atu5520 - acyl-CoA dehydrogenase, at 511,588 to 512,835
Atu5520
Atu5521: Atu5521 - ABC transporter nucleotide binding/ATPase (branched chain amino acid), at 512,862 to 513,641
Atu5521
Atu5522: Atu5522 - hypothetical protein, at 513,714 to 515,120
Atu5522
Atu5523: Atu5523 - ABC transporter membrane spanning protein (branched chain amino acid), at 515,117 to 516,211
Atu5523
Position (kb) 513
514
515
516 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 512.741 kb on + strand at 512.755 kb on - strand at 512.755 kb on - strand at 512.813 kb on + strand at 512.895 kb on - strand at 513.031 kb on + strand, within Atu5521 at 513.068 kb on - strand, within Atu5521 at 513.076 kb on - strand, within Atu5521 at 513.076 kb on - strand, within Atu5521 at 513.092 kb on + strand, within Atu5521 at 513.104 kb on + strand, within Atu5521 at 513.112 kb on - strand, within Atu5521 at 513.112 kb on - strand, within Atu5521 at 513.172 kb on + strand, within Atu5521 at 513.180 kb on - strand, within Atu5521 at 513.182 kb on + strand, within Atu5521 at 513.296 kb on + strand, within Atu5521 at 513.304 kb on - strand, within Atu5521 at 513.305 kb on - strand, within Atu5521 at 513.368 kb on + strand, within Atu5521 at 513.431 kb on + strand, within Atu5521 at 513.431 kb on + strand, within Atu5521 at 513.431 kb on + strand, within Atu5521 at 513.439 kb on - strand, within Atu5521 at 513.439 kb on - strand, within Atu5521 at 513.439 kb on - strand, within Atu5521 at 513.439 kb on - strand, within Atu5521 at 513.596 kb on - strand at 513.621 kb on - strand at 513.650 kb on + strand at 513.745 kb on - strand at 513.745 kb on - strand at 513.829 kb on + strand at 513.885 kb on + strand, within Atu5522 at 513.932 kb on - strand, within Atu5522 at 514.050 kb on - strand, within Atu5522 at 514.077 kb on - strand, within Atu5522 at 514.118 kb on + strand, within Atu5522 at 514.137 kb on - strand, within Atu5522 at 514.140 kb on + strand, within Atu5522 at 514.186 kb on + strand, within Atu5522 at 514.210 kb on + strand, within Atu5522 at 514.210 kb on + strand, within Atu5522 at 514.210 kb on + strand, within Atu5522 at 514.210 kb on + strand, within Atu5522 at 514.210 kb on + strand, within Atu5522 at 514.218 kb on - strand, within Atu5522 at 514.218 kb on - strand, within Atu5522 at 514.218 kb on - strand, within Atu5522 at 514.218 kb on - strand, within Atu5522 at 514.218 kb on - strand, within Atu5522 at 514.218 kb on - strand, within Atu5522 at 514.347 kb on + strand, within Atu5522 at 514.360 kb on - strand, within Atu5522 at 514.410 kb on + strand, within Atu5522 at 514.572 kb on + strand, within Atu5522 at 514.677 kb on + strand, within Atu5522 at 514.684 kb on + strand, within Atu5522 at 514.684 kb on + strand, within Atu5522 at 514.684 kb on + strand, within Atu5522 at 514.684 kb on + strand, within Atu5522 at 514.684 kb on + strand, within Atu5522 at 514.692 kb on - strand, within Atu5522 at 514.739 kb on + strand, within Atu5522 at 514.740 kb on + strand, within Atu5522 at 514.748 kb on - strand, within Atu5522 at 514.835 kb on + strand, within Atu5522 at 514.874 kb on + strand, within Atu5522 at 514.874 kb on + strand, within Atu5522 at 514.882 kb on - strand, within Atu5522 at 514.942 kb on - strand, within Atu5522 at 514.944 kb on + strand, within Atu5522 at 515.001 kb on + strand at 515.018 kb on - strand at 515.023 kb on + strand at 515.023 kb on + strand at 515.031 kb on - strand at 515.031 kb on - strand at 515.031 kb on - strand at 515.058 kb on + strand at 515.103 kb on - strand at 515.147 kb on - strand at 515.246 kb on - strand, within Atu5523 at 515.255 kb on - strand, within Atu5523 at 515.255 kb on - strand, within Atu5523 at 515.261 kb on + strand, within Atu5523 at 515.278 kb on - strand, within Atu5523 at 515.287 kb on + strand, within Atu5523 at 515.290 kb on - strand, within Atu5523 at 515.292 kb on + strand, within Atu5523 at 515.302 kb on + strand, within Atu5523 at 515.310 kb on - strand, within Atu5523 at 515.342 kb on - strand, within Atu5523 at 515.348 kb on - strand, within Atu5523 at 515.478 kb on - strand, within Atu5523 at 515.478 kb on - strand, within Atu5523 at 515.509 kb on - strand, within Atu5523 at 515.574 kb on + strand, within Atu5523 at 515.574 kb on + strand, within Atu5523 at 515.582 kb on - strand, within Atu5523 at 515.668 kb on - strand, within Atu5523 at 515.668 kb on - strand, within Atu5523 at 515.836 kb on - strand, within Atu5523 at 515.862 kb on + strand, within Atu5523 at 515.865 kb on - strand, within Atu5523 at 515.881 kb on - strand, within Atu5523 at 516.019 kb on - strand, within Atu5523 at 516.037 kb on + strand, within Atu5523 at 516.037 kb on + strand, within Atu5523 at 516.037 kb on + strand, within Atu5523 at 516.045 kb on - strand, within Atu5523 at 516.045 kb on - strand, within Atu5523 at 516.045 kb on - strand, within Atu5523 at 516.066 kb on - strand, within Atu5523 at 516.087 kb on + strand, within Atu5523
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=Tobacco; PlantTreatment=None; Sample=leaf; GrowthSubstrate=agar; Collection=Direct; Time=3days remove 512,741 + -2.3 512,755 - -1.7 512,755 - +0.0 512,813 + +0.9 512,895 - -0.7 513,031 + Atu5521 0.22 -0.3 513,068 - Atu5521 0.26 -0.5 513,076 - Atu5521 0.27 +1.0 513,076 - Atu5521 0.27 -0.2 513,092 + Atu5521 0.29 -0.4 513,104 + Atu5521 0.31 -1.8 513,112 - Atu5521 0.32 +0.9 513,112 - Atu5521 0.32 -0.4 513,172 + Atu5521 0.40 +0.2 513,180 - Atu5521 0.41 +1.1 513,182 + Atu5521 0.41 -0.4 513,296 + Atu5521 0.56 +0.4 513,304 - Atu5521 0.57 -0.1 513,305 - Atu5521 0.57 +0.9 513,368 + Atu5521 0.65 -0.1 513,431 + Atu5521 0.73 +0.5 513,431 + Atu5521 0.73 +0.4 513,431 + Atu5521 0.73 +1.9 513,439 - Atu5521 0.74 -0.1 513,439 - Atu5521 0.74 -0.5 513,439 - Atu5521 0.74 +1.4 513,439 - Atu5521 0.74 -1.0 513,596 - -0.7 513,621 - +0.3 513,650 + +0.5 513,745 - -0.1 513,745 - +0.1 513,829 + +1.5 513,885 + Atu5522 0.12 -0.7 513,932 - Atu5522 0.15 +1.0 514,050 - Atu5522 0.24 +0.5 514,077 - Atu5522 0.26 +0.4 514,118 + Atu5522 0.29 +0.5 514,137 - Atu5522 0.30 +0.3 514,140 + Atu5522 0.30 +2.5 514,186 + Atu5522 0.34 +1.5 514,210 + Atu5522 0.35 -1.5 514,210 + Atu5522 0.35 +1.2 514,210 + Atu5522 0.35 +0.9 514,210 + Atu5522 0.35 -0.1 514,210 + Atu5522 0.35 +0.2 514,218 - Atu5522 0.36 -2.0 514,218 - Atu5522 0.36 -0.3 514,218 - Atu5522 0.36 +0.9 514,218 - Atu5522 0.36 -0.1 514,218 - Atu5522 0.36 +0.5 514,218 - Atu5522 0.36 +0.1 514,347 + Atu5522 0.45 +0.5 514,360 - Atu5522 0.46 +0.4 514,410 + Atu5522 0.49 +0.3 514,572 + Atu5522 0.61 -0.3 514,677 + Atu5522 0.68 -1.8 514,684 + Atu5522 0.69 +0.7 514,684 + Atu5522 0.69 +0.6 514,684 + Atu5522 0.69 +0.9 514,684 + Atu5522 0.69 +0.5 514,684 + Atu5522 0.69 +0.5 514,692 - Atu5522 0.70 +1.2 514,739 + Atu5522 0.73 +0.2 514,740 + Atu5522 0.73 +0.0 514,748 - Atu5522 0.73 -0.0 514,835 + Atu5522 0.80 +0.2 514,874 + Atu5522 0.82 -1.9 514,874 + Atu5522 0.82 -0.7 514,882 - Atu5522 0.83 +0.3 514,942 - Atu5522 0.87 -0.6 514,944 + Atu5522 0.87 +0.6 515,001 + -2.6 515,018 - -0.4 515,023 + +0.5 515,023 + -0.5 515,031 - +0.3 515,031 - -1.1 515,031 - -0.2 515,058 + +0.2 515,103 - +0.4 515,147 - +0.7 515,246 - Atu5523 0.12 +0.4 515,255 - Atu5523 0.13 -0.5 515,255 - Atu5523 0.13 -0.8 515,261 + Atu5523 0.13 +0.3 515,278 - Atu5523 0.15 +0.2 515,287 + Atu5523 0.16 -2.3 515,290 - Atu5523 0.16 +1.5 515,292 + Atu5523 0.16 +0.3 515,302 + Atu5523 0.17 +2.5 515,310 - Atu5523 0.18 -3.3 515,342 - Atu5523 0.21 +0.1 515,348 - Atu5523 0.21 +0.4 515,478 - Atu5523 0.33 +0.1 515,478 - Atu5523 0.33 -1.1 515,509 - Atu5523 0.36 -0.4 515,574 + Atu5523 0.42 +0.3 515,574 + Atu5523 0.42 +0.1 515,582 - Atu5523 0.42 -0.7 515,668 - Atu5523 0.50 -0.6 515,668 - Atu5523 0.50 +0.6 515,836 - Atu5523 0.66 -0.3 515,862 + Atu5523 0.68 +0.7 515,865 - Atu5523 0.68 +0.3 515,881 - Atu5523 0.70 +0.3 516,019 - Atu5523 0.82 -0.0 516,037 + Atu5523 0.84 -0.8 516,037 + Atu5523 0.84 +0.4 516,037 + Atu5523 0.84 +0.5 516,045 - Atu5523 0.85 +0.4 516,045 - Atu5523 0.85 +0.3 516,045 - Atu5523 0.85 +0.0 516,066 - Atu5523 0.87 +0.2 516,087 + Atu5523 0.89 +0.7
Or see this region's nucleotide sequence