Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF91

Experiment: 6H2_phage 10 MOI; agar plate assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF89 and GFF90 overlap by 4 nucleotidesGFF90 and GFF91 are separated by 120 nucleotidesGFF91 and GFF92 are separated by 125 nucleotides GFF89 - Poly(A) polymerase (EC 2.7.7.19), at 73,852 to 75,216 GFF89 GFF90 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3), at 75,213 to 75,692 GFF90 GFF91 - 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11), at 75,813 to 76,604 GFF91 GFF92 - Pantoate--beta-alanine ligase (EC 6.3.2.1), at 76,730 to 77,584 GFF92 Position (kb) 75 76 77Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 74.842 kb on + strand, within GFF89at 74.916 kb on + strand, within GFF89at 74.934 kb on + strand, within GFF89at 75.636 kb on - strand, within GFF90at 75.773 kb on - strandat 75.773 kb on - strandat 75.796 kb on + strandat 75.907 kb on + strand, within GFF91at 75.923 kb on + strand, within GFF91at 75.924 kb on - strand, within GFF91at 76.005 kb on - strand, within GFF91at 76.051 kb on + strand, within GFF91at 76.074 kb on - strand, within GFF91at 76.248 kb on - strand, within GFF91at 76.318 kb on + strand, within GFF91at 76.484 kb on + strand, within GFF91at 76.548 kb on + strandat 76.548 kb on + strandat 76.548 kb on + strandat 76.549 kb on - strandat 76.549 kb on - strandat 76.550 kb on + strandat 76.551 kb on - strandat 76.551 kb on - strandat 76.551 kb on - strandat 76.679 kb on + strandat 76.734 kb on + strandat 76.769 kb on - strandat 76.994 kb on + strand, within GFF92at 77.051 kb on + strand, within GFF92at 77.051 kb on + strand, within GFF92at 77.052 kb on - strand, within GFF92at 77.297 kb on - strand, within GFF92at 77.304 kb on - strand, within GFF92at 77.342 kb on + strand, within GFF92at 77.356 kb on + strand, within GFF92at 77.357 kb on - strand, within GFF92at 77.405 kb on - strand, within GFF92

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Per-strain Table

Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay
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74,842 + GFF89 0.73 +0.1
74,916 + GFF89 0.78 -0.2
74,934 + GFF89 0.79 +1.0
75,636 - GFF90 0.88 -3.3
75,773 - -1.4
75,773 - -2.7
75,796 + +1.0
75,907 + GFF91 0.12 +0.3
75,923 + GFF91 0.14 +0.3
75,924 - GFF91 0.14 +0.6
76,005 - GFF91 0.24 +1.4
76,051 + GFF91 0.30 +0.7
76,074 - GFF91 0.33 +0.5
76,248 - GFF91 0.55 +0.9
76,318 + GFF91 0.64 +1.6
76,484 + GFF91 0.85 -0.4
76,548 + -3.8
76,548 + -0.6
76,548 + -0.4
76,549 - +1.6
76,549 - -1.2
76,550 + +1.6
76,551 - +0.0
76,551 - +0.2
76,551 - -0.9
76,679 + +1.3
76,734 + +0.3
76,769 - +0.9
76,994 + GFF92 0.31 +1.4
77,051 + GFF92 0.38 +0.4
77,051 + GFF92 0.38 -0.3
77,052 - GFF92 0.38 +0.2
77,297 - GFF92 0.66 -0.5
77,304 - GFF92 0.67 +0.2
77,342 + GFF92 0.72 +0.7
77,356 + GFF92 0.73 +0.7
77,357 - GFF92 0.73 -3.1
77,405 - GFF92 0.79 -2.6

Or see this region's nucleotide sequence