Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF89

Experiment: 6H2_phage 10 MOI; agar plate assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF88 and GFF89 are separated by 194 nucleotidesGFF89 and GFF90 overlap by 4 nucleotidesGFF90 and GFF91 are separated by 120 nucleotides GFF88 - glutamyl-Q-tRNA synthetase, at 72,716 to 73,657 GFF88 GFF89 - Poly(A) polymerase (EC 2.7.7.19), at 73,852 to 75,216 GFF89 GFF90 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3), at 75,213 to 75,692 GFF90 GFF91 - 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11), at 75,813 to 76,604 GFF91 Position (kb) 73 74 75 76Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 72.852 kb on + strand, within GFF88at 72.853 kb on - strand, within GFF88at 72.853 kb on - strand, within GFF88at 72.988 kb on - strand, within GFF88at 72.988 kb on - strand, within GFF88at 73.059 kb on - strand, within GFF88at 73.059 kb on - strand, within GFF88at 73.059 kb on - strand, within GFF88at 73.160 kb on - strand, within GFF88at 73.276 kb on + strand, within GFF88at 73.373 kb on + strand, within GFF88at 73.374 kb on - strand, within GFF88at 73.375 kb on + strand, within GFF88at 73.626 kb on + strandat 73.720 kb on - strandat 73.720 kb on - strandat 73.792 kb on + strandat 73.797 kb on + strandat 74.326 kb on + strand, within GFF89at 74.605 kb on + strand, within GFF89at 74.655 kb on + strand, within GFF89at 74.842 kb on + strand, within GFF89at 74.916 kb on + strand, within GFF89at 74.934 kb on + strand, within GFF89at 75.636 kb on - strand, within GFF90at 75.773 kb on - strandat 75.773 kb on - strandat 75.796 kb on + strandat 75.907 kb on + strand, within GFF91at 75.923 kb on + strand, within GFF91at 75.924 kb on - strand, within GFF91at 76.005 kb on - strand, within GFF91at 76.051 kb on + strand, within GFF91at 76.074 kb on - strand, within GFF91

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Per-strain Table

Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay
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72,852 + GFF88 0.14 -1.2
72,853 - GFF88 0.15 +0.1
72,853 - GFF88 0.15 +0.8
72,988 - GFF88 0.29 +0.6
72,988 - GFF88 0.29 -1.0
73,059 - GFF88 0.36 -2.0
73,059 - GFF88 0.36 -0.1
73,059 - GFF88 0.36 -0.3
73,160 - GFF88 0.47 +1.5
73,276 + GFF88 0.59 -1.2
73,373 + GFF88 0.70 -2.6
73,374 - GFF88 0.70 +0.9
73,375 + GFF88 0.70 -1.1
73,626 + +0.7
73,720 - -0.7
73,720 - -0.8
73,792 + -0.2
73,797 + +1.7
74,326 + GFF89 0.35 -2.1
74,605 + GFF89 0.55 -4.5
74,655 + GFF89 0.59 +0.4
74,842 + GFF89 0.73 +0.1
74,916 + GFF89 0.78 -0.2
74,934 + GFF89 0.79 +1.0
75,636 - GFF90 0.88 -3.3
75,773 - -1.4
75,773 - -2.7
75,796 + +1.0
75,907 + GFF91 0.12 +0.3
75,923 + GFF91 0.14 +0.3
75,924 - GFF91 0.14 +0.6
76,005 - GFF91 0.24 +1.4
76,051 + GFF91 0.30 +0.7
76,074 - GFF91 0.33 +0.5

Or see this region's nucleotide sequence