Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF84 and GFF85 are separated by 27 nucleotides GFF85 and GFF86 are separated by 16 nucleotides GFF86 and GFF87 are separated by 176 nucleotides GFF87 and GFF88 are separated by 23 nucleotides
GFF84 - ATP-dependent helicase HrpB, at 68,307 to 70,781
GFF84
GFF85 - '2'-5' RNA ligase' transl_table=11, at 70,809 to 71,339
GFF85
GFF86 - Sugar/maltose fermentation stimulation protein homolog, at 71,356 to 72,060
GFF86
GFF87 - C4-type zinc finger protein, DksA/TraR family, at 72,237 to 72,692
GFF87
GFF88 - glutamyl-Q-tRNA synthetase, at 72,716 to 73,657
GFF88
Position (kb) 71
72
73 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 70.428 kb on + strand, within GFF84 at 70.776 kb on + strand at 70.777 kb on - strand at 70.788 kb on - strand at 70.803 kb on - strand at 70.807 kb on + strand at 70.808 kb on + strand at 70.809 kb on - strand at 70.809 kb on - strand at 70.968 kb on - strand, within GFF85 at 71.024 kb on + strand, within GFF85 at 71.029 kb on + strand, within GFF85 at 71.120 kb on + strand, within GFF85 at 71.120 kb on + strand, within GFF85 at 71.186 kb on + strand, within GFF85 at 71.210 kb on + strand, within GFF85 at 71.360 kb on + strand at 71.361 kb on - strand at 71.376 kb on - strand at 71.466 kb on + strand, within GFF86 at 71.503 kb on - strand, within GFF86 at 71.533 kb on - strand, within GFF86 at 71.598 kb on + strand, within GFF86 at 71.670 kb on + strand, within GFF86 at 71.715 kb on - strand, within GFF86 at 71.754 kb on + strand, within GFF86 at 71.755 kb on - strand, within GFF86 at 71.934 kb on + strand, within GFF86 at 71.940 kb on + strand, within GFF86 at 71.943 kb on - strand, within GFF86 at 71.998 kb on + strand at 72.000 kb on + strand at 72.085 kb on + strand at 72.573 kb on + strand, within GFF87 at 72.711 kb on + strand at 72.712 kb on - strand at 72.776 kb on + strand at 72.777 kb on - strand at 72.777 kb on - strand at 72.852 kb on + strand, within GFF88 at 72.853 kb on - strand, within GFF88 at 72.853 kb on - strand, within GFF88 at 72.988 kb on - strand, within GFF88 at 72.988 kb on - strand, within GFF88 at 73.059 kb on - strand, within GFF88 at 73.059 kb on - strand, within GFF88 at 73.059 kb on - strand, within GFF88
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 70,428 + GFF84 0.86 -2.9 70,776 + +0.4 70,777 - +0.6 70,788 - +0.8 70,803 - -2.1 70,807 + +0.9 70,808 + -0.6 70,809 - +1.3 70,809 - -2.0 70,968 - GFF85 0.30 -1.1 71,024 + GFF85 0.40 +0.6 71,029 + GFF85 0.41 +0.2 71,120 + GFF85 0.59 -1.5 71,120 + GFF85 0.59 +1.2 71,186 + GFF85 0.71 -1.2 71,210 + GFF85 0.76 -1.3 71,360 + +1.1 71,361 - -1.2 71,376 - +2.3 71,466 + GFF86 0.16 +1.3 71,503 - GFF86 0.21 +1.1 71,533 - GFF86 0.25 +0.5 71,598 + GFF86 0.34 +1.4 71,670 + GFF86 0.45 -1.2 71,715 - GFF86 0.51 -0.4 71,754 + GFF86 0.56 -4.2 71,755 - GFF86 0.57 +0.6 71,934 + GFF86 0.82 +0.5 71,940 + GFF86 0.83 +0.4 71,943 - GFF86 0.83 -0.1 71,998 + +0.5 72,000 + -0.8 72,085 + -0.5 72,573 + GFF87 0.74 -1.5 72,711 + +0.6 72,712 - +0.4 72,776 + -0.2 72,777 - +1.0 72,777 - -0.1 72,852 + GFF88 0.14 -1.2 72,853 - GFF88 0.15 +0.1 72,853 - GFF88 0.15 +0.8 72,988 - GFF88 0.29 +0.6 72,988 - GFF88 0.29 -1.0 73,059 - GFF88 0.36 -2.0 73,059 - GFF88 0.36 -0.1 73,059 - GFF88 0.36 -0.3
Or see this region's nucleotide sequence