Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF827 and GFF828 are separated by 2 nucleotides GFF828 and GFF829 are separated by 78 nucleotides GFF829 and GFF830 are separated by 102 nucleotides
GFF827 - HflK protein, at 9,800 to 11,059
GFF827
GFF828 - HflC protein, at 11,062 to 12,066
GFF828
GFF829 - Putative inner membrane protein YjeT (clustered with HflC), at 12,145 to 12,342
GFF829
GFF830 - Adenylosuccinate synthetase (EC 6.3.4.4), at 12,445 to 13,743
GFF830
Position (kb)
11
12
13 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 10.063 kb on - strand, within GFF827 at 10.390 kb on + strand, within GFF827 at 10.394 kb on + strand, within GFF827 at 10.468 kb on + strand, within GFF827 at 10.590 kb on - strand, within GFF827 at 10.610 kb on + strand, within GFF827 at 10.610 kb on + strand, within GFF827 at 10.677 kb on - strand, within GFF827 at 10.810 kb on + strand, within GFF827 at 10.821 kb on + strand, within GFF827 at 10.954 kb on + strand at 11.003 kb on - strand at 11.068 kb on - strand at 11.102 kb on + strand at 11.110 kb on + strand at 11.111 kb on - strand at 11.371 kb on + strand, within GFF828 at 11.372 kb on - strand, within GFF828 at 11.558 kb on + strand, within GFF828 at 11.559 kb on - strand, within GFF828 at 11.652 kb on + strand, within GFF828 at 11.731 kb on + strand, within GFF828 at 11.739 kb on + strand, within GFF828 at 11.869 kb on - strand, within GFF828 at 12.131 kb on + strand at 12.133 kb on + strand at 12.134 kb on - strand at 12.317 kb on - strand, within GFF829 at 12.390 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 10,063 - GFF827 0.21 -1.4 10,390 + GFF827 0.47 -0.4 10,394 + GFF827 0.47 -1.3 10,468 + GFF827 0.53 -2.2 10,590 - GFF827 0.63 -0.1 10,610 + GFF827 0.64 -1.9 10,610 + GFF827 0.64 +1.1 10,677 - GFF827 0.70 -1.6 10,810 + GFF827 0.80 -1.2 10,821 + GFF827 0.81 -0.8 10,954 + -2.2 11,003 - -2.1 11,068 - -2.3 11,102 + -3.1 11,110 + -1.5 11,111 - -3.3 11,371 + GFF828 0.31 -0.7 11,372 - GFF828 0.31 -3.4 11,558 + GFF828 0.49 -0.3 11,559 - GFF828 0.49 -1.0 11,652 + GFF828 0.59 -3.4 11,731 + GFF828 0.67 -2.6 11,739 + GFF828 0.67 -2.4 11,869 - GFF828 0.80 -0.8 12,131 + -1.6 12,133 + -0.9 12,134 - -0.4 12,317 - GFF829 0.87 -1.5 12,390 - +0.1
Or see this region's nucleotide sequence