Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF72 and GFF73 are separated by 18 nucleotides GFF73 and GFF74 are separated by 17 nucleotides GFF74 and GFF75 are separated by 85 nucleotides
GFF72 - Minor fimbrial subunit StfE, at 53,828 to 54,340
GFF72
GFF73 - Periplasmic fimbrial chaperone StfD, at 54,359 to 55,111
GFF73
GFF74 - Fimbriae usher protein StfC, at 55,129 to 57,786
GFF74
GFF75 - Major fimbrial subunit StfA, at 57,872 to 58,432
GFF75
Position (kb) 55
56
57
58 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 54.257 kb on - strand, within GFF72 at 54.406 kb on + strand at 54.407 kb on - strand at 54.450 kb on + strand, within GFF73 at 54.589 kb on - strand, within GFF73 at 54.589 kb on - strand, within GFF73 at 54.645 kb on + strand, within GFF73 at 54.649 kb on - strand, within GFF73 at 54.649 kb on - strand, within GFF73 at 54.890 kb on + strand, within GFF73 at 54.945 kb on + strand, within GFF73 at 54.992 kb on - strand, within GFF73 at 55.191 kb on + strand at 55.191 kb on + strand at 55.192 kb on - strand at 55.205 kb on - strand at 55.326 kb on + strand at 55.397 kb on - strand, within GFF74 at 55.427 kb on + strand, within GFF74 at 55.620 kb on - strand, within GFF74 at 55.620 kb on - strand, within GFF74 at 55.620 kb on - strand, within GFF74 at 55.620 kb on - strand, within GFF74 at 55.662 kb on + strand, within GFF74 at 55.662 kb on + strand, within GFF74 at 55.663 kb on - strand, within GFF74 at 55.663 kb on - strand, within GFF74 at 55.663 kb on - strand, within GFF74 at 55.815 kb on + strand, within GFF74 at 55.859 kb on + strand, within GFF74 at 55.860 kb on - strand, within GFF74 at 55.878 kb on - strand, within GFF74 at 55.881 kb on - strand, within GFF74 at 55.955 kb on + strand, within GFF74 at 56.035 kb on - strand, within GFF74 at 56.048 kb on + strand, within GFF74 at 56.048 kb on + strand, within GFF74 at 56.076 kb on - strand, within GFF74 at 56.076 kb on - strand, within GFF74 at 56.156 kb on + strand, within GFF74 at 56.156 kb on + strand, within GFF74 at 56.157 kb on - strand, within GFF74 at 56.198 kb on + strand, within GFF74 at 56.198 kb on + strand, within GFF74 at 56.199 kb on - strand, within GFF74 at 56.207 kb on + strand, within GFF74 at 56.207 kb on + strand, within GFF74 at 56.221 kb on + strand, within GFF74 at 56.244 kb on - strand, within GFF74 at 56.255 kb on + strand, within GFF74 at 56.255 kb on + strand, within GFF74 at 56.256 kb on - strand, within GFF74 at 56.380 kb on + strand, within GFF74 at 56.380 kb on + strand, within GFF74 at 56.381 kb on - strand, within GFF74 at 56.381 kb on - strand, within GFF74 at 56.383 kb on - strand, within GFF74 at 56.403 kb on - strand, within GFF74 at 56.678 kb on + strand, within GFF74 at 56.679 kb on - strand, within GFF74 at 56.732 kb on + strand, within GFF74 at 56.732 kb on + strand, within GFF74 at 56.733 kb on - strand, within GFF74 at 56.774 kb on - strand, within GFF74 at 56.797 kb on - strand, within GFF74 at 56.899 kb on + strand, within GFF74 at 56.900 kb on - strand, within GFF74 at 56.900 kb on - strand, within GFF74 at 56.973 kb on + strand, within GFF74 at 56.973 kb on + strand, within GFF74 at 57.138 kb on + strand, within GFF74 at 57.209 kb on + strand, within GFF74 at 57.210 kb on - strand, within GFF74 at 57.268 kb on + strand, within GFF74 at 57.455 kb on - strand, within GFF74 at 57.506 kb on + strand, within GFF74 at 57.544 kb on - strand at 57.553 kb on - strand at 57.704 kb on + strand at 57.708 kb on + strand at 57.735 kb on + strand at 57.797 kb on - strand at 57.880 kb on - strand at 58.208 kb on - strand, within GFF75
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 54,257 - GFF72 0.84 +1.0 54,406 + -0.3 54,407 - +0.4 54,450 + GFF73 0.12 -3.1 54,589 - GFF73 0.31 +1.1 54,589 - GFF73 0.31 +0.4 54,645 + GFF73 0.38 +0.3 54,649 - GFF73 0.39 +0.3 54,649 - GFF73 0.39 +1.4 54,890 + GFF73 0.71 +0.1 54,945 + GFF73 0.78 +0.5 54,992 - GFF73 0.84 -0.1 55,191 + +0.5 55,191 + +0.2 55,192 - +0.8 55,205 - -0.1 55,326 + -0.2 55,397 - GFF74 0.10 +0.0 55,427 + GFF74 0.11 +1.5 55,620 - GFF74 0.18 +1.7 55,620 - GFF74 0.18 +1.5 55,620 - GFF74 0.18 +0.6 55,620 - GFF74 0.18 -0.9 55,662 + GFF74 0.20 +0.0 55,662 + GFF74 0.20 +0.5 55,663 - GFF74 0.20 -0.6 55,663 - GFF74 0.20 +0.2 55,663 - GFF74 0.20 +0.3 55,815 + GFF74 0.26 +2.4 55,859 + GFF74 0.27 +0.5 55,860 - GFF74 0.28 +0.0 55,878 - GFF74 0.28 -3.0 55,881 - GFF74 0.28 +1.0 55,955 + GFF74 0.31 +3.1 56,035 - GFF74 0.34 +1.3 56,048 + GFF74 0.35 -0.5 56,048 + GFF74 0.35 -0.9 56,076 - GFF74 0.36 +0.8 56,076 - GFF74 0.36 -0.3 56,156 + GFF74 0.39 +0.7 56,156 + GFF74 0.39 -0.2 56,157 - GFF74 0.39 -3.4 56,198 + GFF74 0.40 -0.5 56,198 + GFF74 0.40 +0.5 56,199 - GFF74 0.40 +1.2 56,207 + GFF74 0.41 -0.8 56,207 + GFF74 0.41 +0.7 56,221 + GFF74 0.41 +0.0 56,244 - GFF74 0.42 -0.2 56,255 + GFF74 0.42 -1.7 56,255 + GFF74 0.42 -1.1 56,256 - GFF74 0.42 +1.4 56,380 + GFF74 0.47 +0.1 56,380 + GFF74 0.47 +0.3 56,381 - GFF74 0.47 -0.0 56,381 - GFF74 0.47 +1.2 56,383 - GFF74 0.47 +0.1 56,403 - GFF74 0.48 +0.4 56,678 + GFF74 0.58 +0.4 56,679 - GFF74 0.58 +0.6 56,732 + GFF74 0.60 +0.6 56,732 + GFF74 0.60 -1.0 56,733 - GFF74 0.60 +0.6 56,774 - GFF74 0.62 +0.7 56,797 - GFF74 0.63 -0.5 56,899 + GFF74 0.67 -0.1 56,900 - GFF74 0.67 +1.4 56,900 - GFF74 0.67 -4.1 56,973 + GFF74 0.69 +0.9 56,973 + GFF74 0.69 +1.0 57,138 + GFF74 0.76 +1.8 57,209 + GFF74 0.78 -0.6 57,210 - GFF74 0.78 -0.4 57,268 + GFF74 0.80 +0.5 57,455 - GFF74 0.88 -0.7 57,506 + GFF74 0.89 -0.7 57,544 - +0.8 57,553 - -0.9 57,704 + +2.2 57,708 + +1.0 57,735 + -4.1 57,797 - +0.6 57,880 - +0.9 58,208 - GFF75 0.60 -0.3
Or see this region's nucleotide sequence