Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF654

Experiment: 6H2_phage 10 MOI; agar plate assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF653 and GFF654 are separated by 170 nucleotidesGFF654 and GFF655 are separated by 67 nucleotidesGFF655 and GFF656 are separated by 128 nucleotides GFF653 - Putative inner membrane protein, at 8,884 to 9,792 GFF653 GFF654 - Putative molybdenum transport ATP-binding protein modF, at 9,963 to 11,438 GFF654 GFF655 - DNA-binding domain of ModE / Molybdate-binding domain of ModE, at 11,506 to 12,294 GFF655 GFF656 - Putative exported protein, at 12,423 to 12,572 GFF656 Position (kb) 9 10 11 12Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 8.985 kb on + strand, within GFF653at 8.986 kb on - strand, within GFF653at 9.041 kb on + strand, within GFF653at 9.042 kb on - strand, within GFF653at 9.203 kb on + strand, within GFF653at 9.212 kb on + strand, within GFF653at 9.303 kb on + strand, within GFF653at 9.439 kb on + strand, within GFF653at 9.597 kb on + strand, within GFF653at 9.617 kb on - strand, within GFF653at 10.031 kb on + strandat 10.098 kb on + strandat 10.101 kb on + strandat 10.102 kb on - strandat 10.107 kb on + strandat 10.187 kb on + strand, within GFF654at 10.260 kb on - strand, within GFF654at 10.340 kb on + strand, within GFF654at 10.374 kb on + strand, within GFF654at 10.484 kb on + strand, within GFF654at 10.484 kb on + strand, within GFF654at 10.499 kb on - strand, within GFF654at 10.520 kb on + strand, within GFF654at 10.529 kb on + strand, within GFF654at 10.557 kb on - strand, within GFF654at 10.602 kb on + strand, within GFF654at 10.603 kb on - strand, within GFF654at 10.630 kb on + strand, within GFF654at 10.649 kb on + strand, within GFF654at 10.720 kb on - strand, within GFF654at 10.805 kb on + strand, within GFF654at 10.805 kb on + strand, within GFF654at 10.972 kb on - strand, within GFF654at 10.972 kb on - strand, within GFF654at 11.036 kb on - strand, within GFF654at 11.183 kb on + strand, within GFF654at 11.183 kb on + strand, within GFF654at 11.184 kb on - strand, within GFF654at 11.184 kb on - strand, within GFF654at 11.238 kb on - strand, within GFF654at 11.573 kb on - strandat 12.029 kb on + strand, within GFF655at 12.111 kb on + strand, within GFF655at 12.111 kb on + strand, within GFF655at 12.112 kb on - strand, within GFF655at 12.112 kb on - strand, within GFF655at 12.159 kb on + strand, within GFF655at 12.160 kb on - strand, within GFF655at 12.162 kb on - strand, within GFF655at 12.231 kb on + strandat 12.232 kb on - strandat 12.394 kb on + strandat 12.407 kb on - strandat 12.409 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay
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8,985 + GFF653 0.11 -0.1
8,986 - GFF653 0.11 -0.8
9,041 + GFF653 0.17 +2.5
9,042 - GFF653 0.17 +1.3
9,203 + GFF653 0.35 -0.5
9,212 + GFF653 0.36 -1.1
9,303 + GFF653 0.46 -0.5
9,439 + GFF653 0.61 -3.4
9,597 + GFF653 0.78 +0.5
9,617 - GFF653 0.81 -0.5
10,031 + -2.8
10,098 + -0.3
10,101 + +0.2
10,102 - -0.1
10,107 + +0.2
10,187 + GFF654 0.15 +0.4
10,260 - GFF654 0.20 +0.8
10,340 + GFF654 0.26 +0.3
10,374 + GFF654 0.28 +0.1
10,484 + GFF654 0.35 +1.0
10,484 + GFF654 0.35 +1.3
10,499 - GFF654 0.36 +0.2
10,520 + GFF654 0.38 +1.5
10,529 + GFF654 0.38 +0.8
10,557 - GFF654 0.40 +1.0
10,602 + GFF654 0.43 +0.2
10,603 - GFF654 0.43 +1.9
10,630 + GFF654 0.45 -1.7
10,649 + GFF654 0.46 +0.7
10,720 - GFF654 0.51 -1.0
10,805 + GFF654 0.57 +0.6
10,805 + GFF654 0.57 -0.3
10,972 - GFF654 0.68 +0.6
10,972 - GFF654 0.68 +0.9
11,036 - GFF654 0.73 +1.6
11,183 + GFF654 0.83 -2.7
11,183 + GFF654 0.83 +0.5
11,184 - GFF654 0.83 +0.6
11,184 - GFF654 0.83 -0.4
11,238 - GFF654 0.86 +1.5
11,573 - +0.7
12,029 + GFF655 0.66 -2.5
12,111 + GFF655 0.77 -0.5
12,111 + GFF655 0.77 +1.1
12,112 - GFF655 0.77 -0.9
12,112 - GFF655 0.77 -3.4
12,159 + GFF655 0.83 +0.9
12,160 - GFF655 0.83 +0.2
12,162 - GFF655 0.83 -2.2
12,231 + -1.5
12,232 - +0.7
12,394 + +1.1
12,407 - +0.2
12,409 - -0.6

Or see this region's nucleotide sequence