Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF653 and GFF654 are separated by 170 nucleotides GFF654 and GFF655 are separated by 67 nucleotides GFF655 and GFF656 are separated by 128 nucleotides
GFF653 - Putative inner membrane protein, at 8,884 to 9,792
GFF653
GFF654 - Putative molybdenum transport ATP-binding protein modF, at 9,963 to 11,438
GFF654
GFF655 - DNA-binding domain of ModE / Molybdate-binding domain of ModE, at 11,506 to 12,294
GFF655
GFF656 - Putative exported protein, at 12,423 to 12,572
GFF656
Position (kb)
9
10
11
12 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 8.985 kb on + strand, within GFF653 at 8.986 kb on - strand, within GFF653 at 9.041 kb on + strand, within GFF653 at 9.042 kb on - strand, within GFF653 at 9.203 kb on + strand, within GFF653 at 9.212 kb on + strand, within GFF653 at 9.303 kb on + strand, within GFF653 at 9.439 kb on + strand, within GFF653 at 9.597 kb on + strand, within GFF653 at 9.617 kb on - strand, within GFF653 at 10.031 kb on + strand at 10.098 kb on + strand at 10.101 kb on + strand at 10.102 kb on - strand at 10.107 kb on + strand at 10.187 kb on + strand, within GFF654 at 10.260 kb on - strand, within GFF654 at 10.340 kb on + strand, within GFF654 at 10.374 kb on + strand, within GFF654 at 10.484 kb on + strand, within GFF654 at 10.484 kb on + strand, within GFF654 at 10.499 kb on - strand, within GFF654 at 10.520 kb on + strand, within GFF654 at 10.529 kb on + strand, within GFF654 at 10.557 kb on - strand, within GFF654 at 10.602 kb on + strand, within GFF654 at 10.603 kb on - strand, within GFF654 at 10.630 kb on + strand, within GFF654 at 10.649 kb on + strand, within GFF654 at 10.720 kb on - strand, within GFF654 at 10.805 kb on + strand, within GFF654 at 10.805 kb on + strand, within GFF654 at 10.972 kb on - strand, within GFF654 at 10.972 kb on - strand, within GFF654 at 11.036 kb on - strand, within GFF654 at 11.183 kb on + strand, within GFF654 at 11.183 kb on + strand, within GFF654 at 11.184 kb on - strand, within GFF654 at 11.184 kb on - strand, within GFF654 at 11.238 kb on - strand, within GFF654 at 11.573 kb on - strand at 12.029 kb on + strand, within GFF655 at 12.111 kb on + strand, within GFF655 at 12.111 kb on + strand, within GFF655 at 12.112 kb on - strand, within GFF655 at 12.112 kb on - strand, within GFF655 at 12.159 kb on + strand, within GFF655 at 12.160 kb on - strand, within GFF655 at 12.162 kb on - strand, within GFF655 at 12.231 kb on + strand at 12.232 kb on - strand at 12.394 kb on + strand at 12.407 kb on - strand at 12.409 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 8,985 + GFF653 0.11 -0.1 8,986 - GFF653 0.11 -0.8 9,041 + GFF653 0.17 +2.5 9,042 - GFF653 0.17 +1.3 9,203 + GFF653 0.35 -0.5 9,212 + GFF653 0.36 -1.1 9,303 + GFF653 0.46 -0.5 9,439 + GFF653 0.61 -3.4 9,597 + GFF653 0.78 +0.5 9,617 - GFF653 0.81 -0.5 10,031 + -2.8 10,098 + -0.3 10,101 + +0.2 10,102 - -0.1 10,107 + +0.2 10,187 + GFF654 0.15 +0.4 10,260 - GFF654 0.20 +0.8 10,340 + GFF654 0.26 +0.3 10,374 + GFF654 0.28 +0.1 10,484 + GFF654 0.35 +1.0 10,484 + GFF654 0.35 +1.3 10,499 - GFF654 0.36 +0.2 10,520 + GFF654 0.38 +1.5 10,529 + GFF654 0.38 +0.8 10,557 - GFF654 0.40 +1.0 10,602 + GFF654 0.43 +0.2 10,603 - GFF654 0.43 +1.9 10,630 + GFF654 0.45 -1.7 10,649 + GFF654 0.46 +0.7 10,720 - GFF654 0.51 -1.0 10,805 + GFF654 0.57 +0.6 10,805 + GFF654 0.57 -0.3 10,972 - GFF654 0.68 +0.6 10,972 - GFF654 0.68 +0.9 11,036 - GFF654 0.73 +1.6 11,183 + GFF654 0.83 -2.7 11,183 + GFF654 0.83 +0.5 11,184 - GFF654 0.83 +0.6 11,184 - GFF654 0.83 -0.4 11,238 - GFF654 0.86 +1.5 11,573 - +0.7 12,029 + GFF655 0.66 -2.5 12,111 + GFF655 0.77 -0.5 12,111 + GFF655 0.77 +1.1 12,112 - GFF655 0.77 -0.9 12,112 - GFF655 0.77 -3.4 12,159 + GFF655 0.83 +0.9 12,160 - GFF655 0.83 +0.2 12,162 - GFF655 0.83 -2.2 12,231 + -1.5 12,232 - +0.7 12,394 + +1.1 12,407 - +0.2 12,409 - -0.6
Or see this region's nucleotide sequence