Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF599 and GFF600 are separated by 593 nucleotides GFF600 and GFF601 are separated by 9 nucleotides
GFF599 - Transcriptional activator of maltose regulon, MalT, at 333,474 to 336,179
GFF599
GFF600 - Maltodextrin phosphorylase (EC 2.4.1.1), at 336,773 to 339,166
GFF600
GFF601 - 4-alpha-glucanotransferase (amylomaltase) (EC 2.4.1.25), at 339,176 to 341,254
GFF601
Position (kb) 336
337
338
339
340 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 335.792 kb on - strand, within GFF599 at 335.840 kb on + strand, within GFF599 at 335.841 kb on - strand, within GFF599 at 335.875 kb on + strand, within GFF599 at 335.876 kb on - strand, within GFF599 at 335.947 kb on - strand at 335.947 kb on - strand at 335.947 kb on - strand at 335.947 kb on - strand at 335.947 kb on - strand at 335.995 kb on + strand at 336.052 kb on + strand at 336.134 kb on + strand at 336.159 kb on + strand at 336.242 kb on - strand at 336.247 kb on + strand at 336.292 kb on + strand at 336.462 kb on - strand at 336.470 kb on + strand at 336.592 kb on + strand at 336.627 kb on + strand at 336.637 kb on + strand at 336.637 kb on + strand at 336.637 kb on + strand at 336.638 kb on - strand at 336.659 kb on + strand at 336.659 kb on + strand at 336.843 kb on + strand at 336.963 kb on - strand at 337.044 kb on + strand, within GFF600 at 337.063 kb on + strand, within GFF600 at 337.193 kb on + strand, within GFF600 at 337.366 kb on + strand, within GFF600 at 337.367 kb on - strand, within GFF600 at 337.592 kb on + strand, within GFF600 at 337.592 kb on + strand, within GFF600 at 337.593 kb on - strand, within GFF600 at 337.593 kb on - strand, within GFF600 at 337.673 kb on + strand, within GFF600 at 337.799 kb on + strand, within GFF600 at 337.876 kb on + strand, within GFF600 at 337.877 kb on - strand, within GFF600 at 337.943 kb on - strand, within GFF600 at 337.950 kb on + strand, within GFF600 at 338.387 kb on + strand, within GFF600 at 338.476 kb on - strand, within GFF600 at 338.511 kb on - strand, within GFF600 at 338.561 kb on - strand, within GFF600 at 338.584 kb on + strand, within GFF600 at 338.585 kb on - strand, within GFF600 at 338.585 kb on - strand, within GFF600 at 338.792 kb on - strand, within GFF600 at 338.810 kb on - strand, within GFF600 at 338.895 kb on + strand, within GFF600 at 339.104 kb on - strand at 339.104 kb on - strand at 339.145 kb on + strand at 339.231 kb on - strand at 339.231 kb on - strand at 339.259 kb on + strand at 339.463 kb on + strand, within GFF601 at 339.539 kb on - strand, within GFF601 at 339.621 kb on - strand, within GFF601 at 339.805 kb on - strand, within GFF601 at 339.805 kb on - strand, within GFF601 at 339.805 kb on - strand, within GFF601 at 339.806 kb on + strand, within GFF601 at 339.806 kb on + strand, within GFF601 at 339.807 kb on - strand, within GFF601 at 339.807 kb on - strand, within GFF601 at 339.807 kb on - strand, within GFF601 at 339.807 kb on - strand, within GFF601 at 339.808 kb on + strand, within GFF601 at 339.809 kb on - strand, within GFF601 at 339.809 kb on - strand, within GFF601 at 339.809 kb on - strand, within GFF601 at 339.809 kb on - strand, within GFF601 at 340.080 kb on - strand, within GFF601 at 340.086 kb on + strand, within GFF601 at 340.133 kb on + strand, within GFF601
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 335,792 - GFF599 0.86 +0.6 335,840 + GFF599 0.87 -0.2 335,841 - GFF599 0.87 +0.2 335,875 + GFF599 0.89 -0.8 335,876 - GFF599 0.89 -0.3 335,947 - +0.4 335,947 - +0.9 335,947 - +0.6 335,947 - -2.9 335,947 - +0.3 335,995 + -0.3 336,052 + +0.4 336,134 + -1.9 336,159 + -3.0 336,242 - +1.1 336,247 + -0.2 336,292 + -1.1 336,462 - -1.0 336,470 + +0.2 336,592 + +0.4 336,627 + +0.6 336,637 + +0.6 336,637 + -0.5 336,637 + -0.3 336,638 - +0.3 336,659 + +0.4 336,659 + -2.6 336,843 + -2.8 336,963 - -0.0 337,044 + GFF600 0.11 +1.6 337,063 + GFF600 0.12 +0.6 337,193 + GFF600 0.18 -2.0 337,366 + GFF600 0.25 -3.0 337,367 - GFF600 0.25 +1.1 337,592 + GFF600 0.34 -0.5 337,592 + GFF600 0.34 +0.8 337,593 - GFF600 0.34 -1.0 337,593 - GFF600 0.34 +0.2 337,673 + GFF600 0.38 +0.4 337,799 + GFF600 0.43 -2.6 337,876 + GFF600 0.46 -2.9 337,877 - GFF600 0.46 +0.6 337,943 - GFF600 0.49 -3.8 337,950 + GFF600 0.49 -0.7 338,387 + GFF600 0.67 +0.7 338,476 - GFF600 0.71 +1.8 338,511 - GFF600 0.73 +1.5 338,561 - GFF600 0.75 +1.9 338,584 + GFF600 0.76 +0.6 338,585 - GFF600 0.76 +0.3 338,585 - GFF600 0.76 -0.3 338,792 - GFF600 0.84 -2.6 338,810 - GFF600 0.85 +2.0 338,895 + GFF600 0.89 -0.5 339,104 - -1.0 339,104 - -1.8 339,145 + +0.3 339,231 - +1.1 339,231 - +0.2 339,259 + +1.1 339,463 + GFF601 0.14 -1.9 339,539 - GFF601 0.17 -0.3 339,621 - GFF601 0.21 +0.8 339,805 - GFF601 0.30 +0.0 339,805 - GFF601 0.30 +0.4 339,805 - GFF601 0.30 -0.8 339,806 + GFF601 0.30 +3.5 339,806 + GFF601 0.30 +0.1 339,807 - GFF601 0.30 +0.5 339,807 - GFF601 0.30 +0.3 339,807 - GFF601 0.30 -2.6 339,807 - GFF601 0.30 -0.8 339,808 + GFF601 0.30 +0.1 339,809 - GFF601 0.30 -0.4 339,809 - GFF601 0.30 +1.6 339,809 - GFF601 0.30 -1.6 339,809 - GFF601 0.30 +2.4 340,080 - GFF601 0.43 -0.2 340,086 + GFF601 0.44 +1.0 340,133 + GFF601 0.46 +1.3
Or see this region's nucleotide sequence