Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF57 and GFF58 are separated by 246 nucleotides GFF58 and GFF59 are separated by 88 nucleotides GFF59 and GFF60 are separated by 152 nucleotides
GFF57 - Permeases of the major facilitator superfamily, at 39,837 to 41,138
GFF57
GFF58 - UPF0325 protein YaeH, at 41,385 to 41,771
GFF58
GFF59 - Sugar diacid utilization regulator SdaR, at 41,860 to 43,017
GFF59
GFF60 - HtrA protease/chaperone protein, at 43,170 to 44,597
GFF60
Position (kb) 41
42
43
44 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 40.875 kb on - strand, within GFF57 at 40.878 kb on + strand, within GFF57 at 40.904 kb on + strand, within GFF57 at 40.949 kb on + strand, within GFF57 at 40.950 kb on - strand, within GFF57 at 40.981 kb on - strand, within GFF57 at 41.053 kb on - strand at 41.085 kb on + strand at 41.118 kb on - strand at 41.124 kb on - strand at 41.126 kb on - strand at 41.139 kb on - strand at 41.234 kb on + strand at 41.238 kb on + strand at 41.239 kb on - strand at 41.239 kb on - strand at 41.496 kb on - strand, within GFF58 at 41.528 kb on + strand, within GFF58 at 41.529 kb on - strand, within GFF58 at 41.533 kb on - strand, within GFF58 at 41.581 kb on - strand, within GFF58 at 41.582 kb on + strand, within GFF58 at 41.582 kb on + strand, within GFF58 at 41.723 kb on + strand, within GFF58 at 41.766 kb on - strand at 41.819 kb on + strand at 41.821 kb on + strand at 41.821 kb on + strand at 41.822 kb on - strand at 41.823 kb on + strand at 41.841 kb on - strand at 41.892 kb on - strand at 41.892 kb on - strand at 41.994 kb on + strand, within GFF59 at 41.995 kb on - strand, within GFF59 at 42.049 kb on + strand, within GFF59 at 42.050 kb on - strand, within GFF59 at 42.050 kb on - strand, within GFF59 at 42.052 kb on + strand, within GFF59 at 42.167 kb on + strand, within GFF59 at 42.240 kb on + strand, within GFF59 at 42.260 kb on + strand, within GFF59 at 42.690 kb on + strand, within GFF59 at 42.690 kb on + strand, within GFF59 at 42.712 kb on - strand, within GFF59 at 42.712 kb on - strand, within GFF59 at 42.772 kb on + strand, within GFF59 at 42.888 kb on + strand, within GFF59 at 42.935 kb on - strand at 42.964 kb on - strand at 42.979 kb on - strand at 42.979 kb on - strand at 43.047 kb on + strand at 43.098 kb on + strand at 43.098 kb on + strand at 43.098 kb on + strand at 43.099 kb on - strand at 43.102 kb on + strand at 43.171 kb on + strand at 43.190 kb on + strand at 43.197 kb on - strand at 43.197 kb on - strand at 43.526 kb on + strand, within GFF60 at 43.785 kb on - strand, within GFF60 at 43.942 kb on - strand, within GFF60
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 40,875 - GFF57 0.80 -2.0 40,878 + GFF57 0.80 -0.6 40,904 + GFF57 0.82 +0.8 40,949 + GFF57 0.85 +0.8 40,950 - GFF57 0.85 -0.3 40,981 - GFF57 0.88 +0.5 41,053 - +1.0 41,085 + -0.2 41,118 - +0.6 41,124 - -0.2 41,126 - +0.3 41,139 - -0.7 41,234 + -0.6 41,238 + +0.2 41,239 - -0.2 41,239 - -0.6 41,496 - GFF58 0.29 -1.1 41,528 + GFF58 0.37 -0.3 41,529 - GFF58 0.37 -0.7 41,533 - GFF58 0.38 -0.1 41,581 - GFF58 0.51 +0.4 41,582 + GFF58 0.51 +0.5 41,582 + GFF58 0.51 -3.1 41,723 + GFF58 0.87 -1.1 41,766 - -3.9 41,819 + -1.7 41,821 + -0.5 41,821 + +0.4 41,822 - -0.2 41,823 + -1.4 41,841 - -0.9 41,892 - +0.7 41,892 - +0.4 41,994 + GFF59 0.12 -1.4 41,995 - GFF59 0.12 +0.4 42,049 + GFF59 0.16 +1.1 42,050 - GFF59 0.16 +0.6 42,050 - GFF59 0.16 +0.1 42,052 + GFF59 0.17 -0.8 42,167 + GFF59 0.27 +0.6 42,240 + GFF59 0.33 +0.1 42,260 + GFF59 0.35 +0.2 42,690 + GFF59 0.72 -1.8 42,690 + GFF59 0.72 +1.3 42,712 - GFF59 0.74 +0.7 42,712 - GFF59 0.74 -0.0 42,772 + GFF59 0.79 +0.6 42,888 + GFF59 0.89 -0.3 42,935 - -3.0 42,964 - +0.0 42,979 - -0.2 42,979 - -0.9 43,047 + +0.2 43,098 + +0.5 43,098 + -1.8 43,098 + +0.4 43,099 - -1.9 43,102 + +0.3 43,171 + -0.4 43,190 + +1.7 43,197 - +0.8 43,197 - +0.0 43,526 + GFF60 0.25 +0.4 43,785 - GFF60 0.43 -1.8 43,942 - GFF60 0.54 -0.6
Or see this region's nucleotide sequence