Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF525 and GFF526 overlap by 4 nucleotides GFF526 and GFF527 overlap by 1 nucleotides
GFF525 - Putative membrane protein, at 256,315 to 257,382
GFF525
GFF526 - ABC-type multidrug transport system, permease component, at 257,379 to 260,120
GFF526
GFF527 - FIG01045128: hypothetical protein, at 260,120 to 261,244
GFF527
Position (kb)
257
258
259
260
261 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 256.763 kb on + strand, within GFF525 at 256.870 kb on - strand, within GFF525 at 256.875 kb on + strand, within GFF525 at 256.878 kb on + strand, within GFF525 at 256.956 kb on - strand, within GFF525 at 257.061 kb on + strand, within GFF525 at 257.062 kb on - strand, within GFF525 at 257.152 kb on - strand, within GFF525 at 257.290 kb on + strand at 257.360 kb on + strand at 257.469 kb on - strand at 257.493 kb on - strand at 257.493 kb on - strand at 257.679 kb on + strand, within GFF526 at 257.944 kb on + strand, within GFF526 at 257.944 kb on + strand, within GFF526 at 257.954 kb on + strand, within GFF526 at 258.000 kb on + strand, within GFF526 at 258.001 kb on - strand, within GFF526 at 258.002 kb on + strand, within GFF526 at 258.158 kb on + strand, within GFF526 at 258.180 kb on + strand, within GFF526 at 258.435 kb on + strand, within GFF526 at 258.436 kb on - strand, within GFF526 at 258.437 kb on + strand, within GFF526 at 258.437 kb on + strand, within GFF526 at 258.438 kb on - strand, within GFF526 at 258.438 kb on - strand, within GFF526 at 258.438 kb on - strand, within GFF526 at 258.459 kb on + strand, within GFF526 at 258.563 kb on + strand, within GFF526 at 258.584 kb on + strand, within GFF526 at 258.725 kb on + strand, within GFF526 at 258.725 kb on + strand, within GFF526 at 259.020 kb on + strand, within GFF526 at 259.021 kb on - strand, within GFF526 at 259.106 kb on + strand, within GFF526 at 259.114 kb on - strand, within GFF526 at 259.125 kb on - strand, within GFF526 at 259.245 kb on + strand, within GFF526 at 259.401 kb on + strand, within GFF526 at 259.402 kb on - strand, within GFF526 at 259.639 kb on + strand, within GFF526 at 259.797 kb on + strand, within GFF526 at 259.797 kb on + strand, within GFF526 at 259.881 kb on - strand at 259.883 kb on + strand at 260.044 kb on + strand at 260.048 kb on + strand at 260.049 kb on - strand at 260.134 kb on + strand at 260.138 kb on - strand at 260.138 kb on - strand at 260.228 kb on + strand at 260.239 kb on + strand, within GFF527 at 260.323 kb on + strand, within GFF527 at 260.331 kb on - strand, within GFF527 at 260.404 kb on + strand, within GFF527 at 260.510 kb on - strand, within GFF527 at 260.522 kb on + strand, within GFF527 at 260.523 kb on - strand, within GFF527 at 260.523 kb on - strand, within GFF527 at 260.523 kb on - strand, within GFF527 at 260.524 kb on + strand, within GFF527 at 260.533 kb on + strand, within GFF527 at 260.539 kb on + strand, within GFF527 at 260.582 kb on - strand, within GFF527 at 260.621 kb on - strand, within GFF527 at 260.677 kb on + strand, within GFF527 at 260.678 kb on - strand, within GFF527 at 260.678 kb on - strand, within GFF527 at 260.692 kb on - strand, within GFF527 at 260.740 kb on - strand, within GFF527 at 260.751 kb on + strand, within GFF527 at 260.751 kb on + strand, within GFF527 at 260.985 kb on + strand, within GFF527 at 260.985 kb on + strand, within GFF527 at 260.988 kb on + strand, within GFF527 at 260.988 kb on + strand, within GFF527 at 261.013 kb on - strand, within GFF527 at 261.050 kb on - strand, within GFF527 at 261.070 kb on + strand, within GFF527 at 261.073 kb on + strand, within GFF527
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 256,763 + GFF525 0.42 -0.2 256,870 - GFF525 0.52 -4.4 256,875 + GFF525 0.52 +1.0 256,878 + GFF525 0.53 +0.1 256,956 - GFF525 0.60 -2.6 257,061 + GFF525 0.70 +0.5 257,062 - GFF525 0.70 +0.2 257,152 - GFF525 0.78 +2.0 257,290 + -0.3 257,360 + +0.6 257,469 - -2.2 257,493 - +0.5 257,493 - -1.4 257,679 + GFF526 0.11 +0.1 257,944 + GFF526 0.21 +0.3 257,944 + GFF526 0.21 -0.7 257,954 + GFF526 0.21 +0.2 258,000 + GFF526 0.23 -0.1 258,001 - GFF526 0.23 -2.2 258,002 + GFF526 0.23 +0.3 258,158 + GFF526 0.28 +2.8 258,180 + GFF526 0.29 -0.6 258,435 + GFF526 0.39 -0.6 258,436 - GFF526 0.39 +0.9 258,437 + GFF526 0.39 +0.7 258,437 + GFF526 0.39 +2.1 258,438 - GFF526 0.39 +2.0 258,438 - GFF526 0.39 +2.3 258,438 - GFF526 0.39 +1.1 258,459 + GFF526 0.39 +0.0 258,563 + GFF526 0.43 -2.8 258,584 + GFF526 0.44 +1.4 258,725 + GFF526 0.49 +0.1 258,725 + GFF526 0.49 +0.3 259,020 + GFF526 0.60 -0.2 259,021 - GFF526 0.60 +1.3 259,106 + GFF526 0.63 +0.6 259,114 - GFF526 0.63 +0.9 259,125 - GFF526 0.64 +1.7 259,245 + GFF526 0.68 -0.1 259,401 + GFF526 0.74 +1.3 259,402 - GFF526 0.74 +0.2 259,639 + GFF526 0.82 -0.0 259,797 + GFF526 0.88 +1.0 259,797 + GFF526 0.88 -1.4 259,881 - +0.9 259,883 + +0.8 260,044 + +0.7 260,048 + -0.8 260,049 - -4.3 260,134 + +0.3 260,138 - +0.0 260,138 - -0.3 260,228 + -0.3 260,239 + GFF527 0.11 -2.2 260,323 + GFF527 0.18 -3.3 260,331 - GFF527 0.19 +0.5 260,404 + GFF527 0.25 +1.0 260,510 - GFF527 0.35 -0.6 260,522 + GFF527 0.36 -0.5 260,523 - GFF527 0.36 -0.7 260,523 - GFF527 0.36 -0.1 260,523 - GFF527 0.36 +1.0 260,524 + GFF527 0.36 -1.2 260,533 + GFF527 0.37 -2.8 260,539 + GFF527 0.37 +0.0 260,582 - GFF527 0.41 +0.7 260,621 - GFF527 0.45 -0.7 260,677 + GFF527 0.50 +0.5 260,678 - GFF527 0.50 +0.6 260,678 - GFF527 0.50 +1.9 260,692 - GFF527 0.51 +1.2 260,740 - GFF527 0.55 -0.8 260,751 + GFF527 0.56 -0.3 260,751 + GFF527 0.56 +0.8 260,985 + GFF527 0.77 +0.2 260,985 + GFF527 0.77 +0.8 260,988 + GFF527 0.77 +0.4 260,988 + GFF527 0.77 +0.8 261,013 - GFF527 0.79 -0.6 261,050 - GFF527 0.83 +0.5 261,070 + GFF527 0.84 -1.0 261,073 + GFF527 0.85 -3.8
Or see this region's nucleotide sequence