Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4638 and GFF4639 are separated by 523 nucleotides GFF4639 and GFF4640 are separated by 14 nucleotides
GFF4638 - Succinate dehydrogenase iron-sulfur protein (EC 1.3.99.1), at 431,046 to 431,765
GFF4638
GFF4639 - 2-oxoglutarate dehydrogenase E1 component (EC 1.2.4.2), at 432,289 to 435,090
GFF4639
GFF4640 - Dihydrolipoamide succinyltransferase component (E2) of 2-oxoglutarate dehydrogenase complex (EC 2.3.1.61), at 435,105 to 436,313
GFF4640
Position (kb) 432
433
434
435
436 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 431.495 kb on + strand, within GFF4638 at 431.495 kb on + strand, within GFF4638 at 431.495 kb on + strand, within GFF4638 at 431.735 kb on + strand at 431.736 kb on - strand at 431.736 kb on - strand at 431.763 kb on + strand at 431.767 kb on + strand at 431.795 kb on + strand at 431.795 kb on + strand at 431.900 kb on - strand at 431.903 kb on - strand at 431.957 kb on + strand at 431.957 kb on + strand at 431.957 kb on + strand at 431.958 kb on - strand at 431.961 kb on + strand at 431.961 kb on + strand at 432.098 kb on - strand at 432.114 kb on + strand at 432.248 kb on + strand at 432.249 kb on - strand at 432.804 kb on + strand, within GFF4639 at 433.870 kb on - strand, within GFF4639 at 434.244 kb on + strand, within GFF4639 at 434.565 kb on + strand, within GFF4639 at 434.566 kb on - strand, within GFF4639 at 435.030 kb on + strand at 435.882 kb on + strand, within GFF4640
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 431,495 + GFF4638 0.62 -1.5 431,495 + GFF4638 0.62 -2.5 431,495 + GFF4638 0.62 +0.0 431,735 + -1.9 431,736 - -3.2 431,736 - -1.2 431,763 + -1.6 431,767 + +0.1 431,795 + -1.2 431,795 + -0.3 431,900 - -2.6 431,903 - -1.5 431,957 + +1.2 431,957 + +0.9 431,957 + -0.8 431,958 - -3.4 431,961 + -0.6 431,961 + -2.4 432,098 - -3.0 432,114 + -2.6 432,248 + -1.4 432,249 - -1.7 432,804 + GFF4639 0.18 -2.9 433,870 - GFF4639 0.56 -0.4 434,244 + GFF4639 0.70 -3.4 434,565 + GFF4639 0.81 -1.9 434,566 - GFF4639 0.81 -1.7 435,030 + -2.6 435,882 + GFF4640 0.64 -1.2
Or see this region's nucleotide sequence