Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF459 and GFF460 are separated by 51 nucleotides GFF460 and GFF461 overlap by 16 nucleotides GFF461 and GFF462 overlap by 32 nucleotides GFF462 and GFF463 overlap by 23 nucleotides
GFF459 - Outer membrane protein A precursor, at 179,610 to 180,272
GFF459
GFF460 - hypothetical protein, at 180,324 to 180,440
GFF460
GFF461 - Anaerobic dimethyl sulfoxide reductase chain A (EC 1.8.5.3), at 180,425 to 182,758
GFF461
GFF462 - Acetyltransferase (EC 2.3.1.-), at 182,727 to 183,167
GFF462
GFF463 - DNA-3-methyladenine glycosylase (EC 3.2.2.20), at 183,145 to 183,726
GFF463
Position (kb)
180
181
182
183 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 179.446 kb on + strand at 179.504 kb on - strand at 179.511 kb on + strand at 179.566 kb on - strand at 179.606 kb on + strand at 179.611 kb on + strand at 179.611 kb on + strand at 179.657 kb on + strand at 179.657 kb on + strand at 179.936 kb on + strand, within GFF459 at 179.957 kb on + strand, within GFF459 at 179.957 kb on + strand, within GFF459 at 179.958 kb on - strand, within GFF459 at 179.958 kb on - strand, within GFF459 at 180.034 kb on + strand, within GFF459 at 180.034 kb on + strand, within GFF459 at 180.034 kb on + strand, within GFF459 at 180.034 kb on + strand, within GFF459 at 180.035 kb on - strand, within GFF459 at 180.035 kb on - strand, within GFF459 at 180.092 kb on + strand, within GFF459 at 180.174 kb on - strand, within GFF459 at 180.219 kb on + strand at 180.220 kb on - strand at 180.259 kb on - strand at 180.259 kb on - strand at 180.284 kb on - strand at 180.378 kb on - strand, within GFF460 at 180.583 kb on + strand at 180.614 kb on - strand at 180.652 kb on - strand at 180.652 kb on - strand at 180.737 kb on + strand, within GFF461 at 180.767 kb on + strand, within GFF461 at 180.789 kb on + strand, within GFF461 at 180.805 kb on + strand, within GFF461 at 180.821 kb on + strand, within GFF461 at 180.842 kb on + strand, within GFF461 at 180.842 kb on + strand, within GFF461 at 180.842 kb on + strand, within GFF461 at 180.886 kb on + strand, within GFF461 at 180.896 kb on + strand, within GFF461 at 180.897 kb on - strand, within GFF461 at 180.920 kb on + strand, within GFF461 at 181.505 kb on + strand, within GFF461 at 181.615 kb on + strand, within GFF461 at 181.694 kb on - strand, within GFF461 at 181.720 kb on + strand, within GFF461 at 181.843 kb on + strand, within GFF461 at 181.844 kb on - strand, within GFF461 at 181.848 kb on - strand, within GFF461 at 181.848 kb on - strand, within GFF461 at 181.880 kb on + strand, within GFF461 at 181.881 kb on - strand, within GFF461 at 181.881 kb on - strand, within GFF461 at 181.882 kb on + strand, within GFF461 at 181.882 kb on + strand, within GFF461 at 181.882 kb on + strand, within GFF461 at 181.883 kb on - strand, within GFF461 at 181.883 kb on - strand, within GFF461 at 181.992 kb on - strand, within GFF461 at 182.253 kb on - strand, within GFF461 at 182.253 kb on - strand, within GFF461 at 182.378 kb on - strand, within GFF461 at 182.381 kb on + strand, within GFF461 at 182.381 kb on + strand, within GFF461 at 182.382 kb on - strand, within GFF461 at 182.757 kb on - strand at 183.034 kb on - strand, within GFF462 at 183.037 kb on + strand, within GFF462 at 183.100 kb on + strand, within GFF462 at 183.101 kb on - strand, within GFF462 at 183.101 kb on - strand, within GFF462 at 183.131 kb on + strand at 183.131 kb on + strand at 183.132 kb on - strand at 183.202 kb on - strand at 183.202 kb on - strand at 183.231 kb on + strand, within GFF463 at 183.516 kb on + strand, within GFF463 at 183.517 kb on - strand, within GFF463 at 183.687 kb on + strand at 183.687 kb on + strand at 183.687 kb on + strand at 183.688 kb on - strand at 183.689 kb on + strand at 183.689 kb on + strand at 183.690 kb on - strand at 183.690 kb on - strand at 183.690 kb on - strand at 183.726 kb on + strand at 183.727 kb on - strand at 183.727 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 179,446 + +0.2 179,504 - +1.2 179,511 + +1.2 179,566 - -3.4 179,606 + -1.6 179,611 + +0.4 179,611 + +0.4 179,657 + +2.0 179,657 + -0.6 179,936 + GFF459 0.49 +0.4 179,957 + GFF459 0.52 -0.2 179,957 + GFF459 0.52 -0.5 179,958 - GFF459 0.52 -1.6 179,958 - GFF459 0.52 -1.3 180,034 + GFF459 0.64 +2.1 180,034 + GFF459 0.64 -0.4 180,034 + GFF459 0.64 +0.6 180,034 + GFF459 0.64 -0.6 180,035 - GFF459 0.64 -0.5 180,035 - GFF459 0.64 +1.1 180,092 + GFF459 0.73 -0.2 180,174 - GFF459 0.85 +0.3 180,219 + -4.5 180,220 - +0.9 180,259 - -0.6 180,259 - +0.3 180,284 - +0.7 180,378 - GFF460 0.46 +0.5 180,583 + -5.5 180,614 - +0.2 180,652 - +1.2 180,652 - -1.9 180,737 + GFF461 0.13 +0.6 180,767 + GFF461 0.15 +0.5 180,789 + GFF461 0.16 +1.2 180,805 + GFF461 0.16 +0.4 180,821 + GFF461 0.17 -2.8 180,842 + GFF461 0.18 +0.9 180,842 + GFF461 0.18 +0.9 180,842 + GFF461 0.18 -0.5 180,886 + GFF461 0.20 +0.9 180,896 + GFF461 0.20 +0.7 180,897 - GFF461 0.20 -0.0 180,920 + GFF461 0.21 +1.0 181,505 + GFF461 0.46 +0.2 181,615 + GFF461 0.51 +1.0 181,694 - GFF461 0.54 +0.7 181,720 + GFF461 0.55 +1.4 181,843 + GFF461 0.61 +0.6 181,844 - GFF461 0.61 -0.2 181,848 - GFF461 0.61 +0.2 181,848 - GFF461 0.61 -1.0 181,880 + GFF461 0.62 +1.3 181,881 - GFF461 0.62 -2.4 181,881 - GFF461 0.62 +0.8 181,882 + GFF461 0.62 -1.0 181,882 + GFF461 0.62 +1.4 181,882 + GFF461 0.62 +0.5 181,883 - GFF461 0.62 -0.6 181,883 - GFF461 0.62 +0.6 181,992 - GFF461 0.67 -0.5 182,253 - GFF461 0.78 -0.1 182,253 - GFF461 0.78 +0.5 182,378 - GFF461 0.84 -0.2 182,381 + GFF461 0.84 +1.1 182,381 + GFF461 0.84 +0.6 182,382 - GFF461 0.84 -0.2 182,757 - +1.0 183,034 - GFF462 0.70 +1.2 183,037 + GFF462 0.70 -0.5 183,100 + GFF462 0.85 -0.3 183,101 - GFF462 0.85 -3.9 183,101 - GFF462 0.85 +1.7 183,131 + +1.6 183,131 + -0.1 183,132 - -1.9 183,202 - +0.9 183,202 - +0.5 183,231 + GFF463 0.15 -0.1 183,516 + GFF463 0.64 +0.1 183,517 - GFF463 0.64 +0.1 183,687 + -1.8 183,687 + +0.4 183,687 + -0.8 183,688 - +0.1 183,689 + +0.4 183,689 + -0.4 183,690 - +0.7 183,690 - +1.0 183,690 - -0.5 183,726 + -1.0 183,727 - +0.2 183,727 - -0.0
Or see this region's nucleotide sequence