Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4569 and GFF4570 are separated by 116 nucleotides GFF4570 and GFF4571 overlap by 1 nucleotides GFF4571 and GFF4572 overlap by 1 nucleotides
GFF4569 - Inosine-uridine preferring nucleoside hydrolase (EC 3.2.2.1), at 355,772 to 356,707
GFF4569
GFF4570 - Glutamate Aspartate transport ATP-binding protein GltL (TC 3.A.1.3.4), at 356,824 to 357,549
GFF4570
GFF4571 - Glutamate Aspartate transport system permease protein GltK (TC 3.A.1.3.4), at 357,549 to 358,223
GFF4571
GFF4572 - Glutamate Aspartate transport system permease protein GltJ (TC 3.A.1.3.4), at 358,223 to 358,963
GFF4572
Position (kb) 356
357
358 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 355.872 kb on + strand, within GFF4569 at 355.902 kb on + strand, within GFF4569 at 356.038 kb on + strand, within GFF4569 at 356.129 kb on - strand, within GFF4569 at 356.206 kb on - strand, within GFF4569 at 356.237 kb on - strand, within GFF4569 at 356.285 kb on - strand, within GFF4569 at 356.690 kb on - strand at 356.720 kb on + strand at 356.728 kb on - strand at 356.780 kb on + strand at 356.913 kb on + strand, within GFF4570 at 357.017 kb on - strand, within GFF4570 at 357.074 kb on - strand, within GFF4570 at 357.093 kb on + strand, within GFF4570 at 357.093 kb on + strand, within GFF4570 at 357.094 kb on - strand, within GFF4570 at 357.094 kb on - strand, within GFF4570 at 357.094 kb on - strand, within GFF4570 at 357.305 kb on - strand, within GFF4570 at 357.461 kb on - strand, within GFF4570 at 357.571 kb on + strand at 357.611 kb on - strand at 357.730 kb on - strand, within GFF4571 at 357.789 kb on - strand, within GFF4571 at 358.049 kb on - strand, within GFF4571 at 358.180 kb on + strand at 358.288 kb on + strand at 358.308 kb on + strand, within GFF4572 at 358.308 kb on + strand, within GFF4572 at 358.309 kb on - strand, within GFF4572 at 358.498 kb on - strand, within GFF4572
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 355,872 + GFF4569 0.11 +2.5 355,902 + GFF4569 0.14 +0.6 356,038 + GFF4569 0.28 -2.2 356,129 - GFF4569 0.38 -2.9 356,206 - GFF4569 0.46 +1.1 356,237 - GFF4569 0.50 +0.3 356,285 - GFF4569 0.55 +0.8 356,690 - +0.4 356,720 + -0.1 356,728 - -1.7 356,780 + -1.7 356,913 + GFF4570 0.12 -2.8 357,017 - GFF4570 0.27 +1.9 357,074 - GFF4570 0.34 -0.2 357,093 + GFF4570 0.37 -1.4 357,093 + GFF4570 0.37 -2.2 357,094 - GFF4570 0.37 -0.1 357,094 - GFF4570 0.37 -2.2 357,094 - GFF4570 0.37 +1.3 357,305 - GFF4570 0.66 -0.7 357,461 - GFF4570 0.88 -0.8 357,571 + -0.6 357,611 - -0.9 357,730 - GFF4571 0.27 +0.3 357,789 - GFF4571 0.36 -3.4 358,049 - GFF4571 0.74 +0.8 358,180 + -1.2 358,288 + -2.1 358,308 + GFF4572 0.11 -0.5 358,308 + GFF4572 0.11 -0.6 358,309 - GFF4572 0.12 -0.1 358,498 - GFF4572 0.37 -0.0
Or see this region's nucleotide sequence