Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4548

Experiment: 6H2_phage 10 MOI; agar plate assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4547 and GFF4548 are separated by 2 nucleotidesGFF4548 and GFF4549 are separated by 30 nucleotidesGFF4549 and GFF4550 are separated by 3 nucleotides GFF4547 - Rod shape-determining protein RodA, at 331,122 to 332,234 GFF4547 GFF4548 - Penicillin-binding protein 2 (PBP-2), at 332,237 to 334,138 GFF4548 GFF4549 - LSU m3Psi1915 methyltransferase RlmH, at 334,169 to 334,636 GFF4549 GFF4550 - Iojap protein, at 334,640 to 334,957 GFF4550 Position (kb) 332 333 334 335Strain fitness (log2 ratio) -1 0 1at 334.148 kb on - strandat 334.263 kb on - strand, within GFF4549at 334.386 kb on - strand, within GFF4549at 334.570 kb on - strand, within GFF4549at 334.808 kb on - strand, within GFF4550at 334.814 kb on - strand, within GFF4550at 334.850 kb on - strand, within GFF4550at 334.884 kb on - strand, within GFF4550at 334.884 kb on - strand, within GFF4550at 335.066 kb on - strandat 335.069 kb on - strandat 335.099 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay
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334,148 - -0.5
334,263 - GFF4549 0.20 +0.5
334,386 - GFF4549 0.46 -0.3
334,570 - GFF4549 0.86 +1.3
334,808 - GFF4550 0.53 +0.3
334,814 - GFF4550 0.55 +0.5
334,850 - GFF4550 0.66 -0.7
334,884 - GFF4550 0.77 +0.7
334,884 - GFF4550 0.77 -1.7
335,066 - +0.9
335,069 - +0.6
335,099 - -0.0

Or see this region's nucleotide sequence