Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4532 and GFF4533 overlap by 32 nucleotides GFF4533 and GFF4534 are separated by 53 nucleotides GFF4534 and GFF4535 are separated by 432 nucleotides
GFF4532 - Sensor kinase CitA, DpiB (EC 2.7.3.-), at 318,241 to 319,902
GFF4532
GFF4533 - Transcriptional regulatory protein CitB, DpiA, at 319,871 to 320,551
GFF4533
GFF4534 - C4-dicarboxylate transporter DcuC (TC 2.A.61.1.1), at 320,605 to 321,948
GFF4534
GFF4535 - Lipid A acylation protein PagP, palmitoyltransferase, at 322,381 to 322,941
GFF4535
Position (kb) 320
321
322 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 319.649 kb on + strand, within GFF4532 at 319.718 kb on + strand, within GFF4532 at 319.718 kb on + strand, within GFF4532 at 319.719 kb on - strand, within GFF4532 at 319.719 kb on - strand, within GFF4532 at 319.719 kb on - strand, within GFF4532 at 319.719 kb on - strand, within GFF4532 at 319.719 kb on - strand, within GFF4532 at 319.891 kb on - strand at 319.938 kb on - strand at 320.029 kb on - strand, within GFF4533 at 320.041 kb on + strand, within GFF4533 at 320.045 kb on + strand, within GFF4533 at 320.195 kb on - strand, within GFF4533 at 320.286 kb on + strand, within GFF4533 at 320.286 kb on + strand, within GFF4533 at 320.503 kb on + strand at 320.533 kb on + strand at 320.533 kb on + strand at 320.816 kb on + strand, within GFF4534 at 320.850 kb on + strand, within GFF4534 at 320.929 kb on + strand, within GFF4534 at 320.946 kb on + strand, within GFF4534 at 320.947 kb on - strand, within GFF4534 at 321.177 kb on + strand, within GFF4534 at 321.204 kb on + strand, within GFF4534 at 321.204 kb on + strand, within GFF4534 at 321.322 kb on - strand, within GFF4534 at 321.552 kb on + strand, within GFF4534 at 321.653 kb on - strand, within GFF4534 at 321.653 kb on - strand, within GFF4534 at 321.661 kb on + strand, within GFF4534 at 321.662 kb on - strand, within GFF4534 at 321.690 kb on + strand, within GFF4534 at 321.691 kb on - strand, within GFF4534 at 321.714 kb on + strand, within GFF4534 at 321.850 kb on - strand at 322.373 kb on - strand at 322.531 kb on + strand, within GFF4535 at 322.531 kb on + strand, within GFF4535 at 322.664 kb on - strand, within GFF4535 at 322.838 kb on + strand, within GFF4535 at 322.852 kb on + strand, within GFF4535 at 322.897 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 319,649 + GFF4532 0.85 -1.2 319,718 + GFF4532 0.89 -2.5 319,718 + GFF4532 0.89 +0.9 319,719 - GFF4532 0.89 -1.1 319,719 - GFF4532 0.89 -1.8 319,719 - GFF4532 0.89 -1.7 319,719 - GFF4532 0.89 +0.2 319,719 - GFF4532 0.89 +1.0 319,891 - -0.2 319,938 - -0.4 320,029 - GFF4533 0.23 +0.8 320,041 + GFF4533 0.25 +0.9 320,045 + GFF4533 0.26 +0.8 320,195 - GFF4533 0.48 +0.6 320,286 + GFF4533 0.61 +1.8 320,286 + GFF4533 0.61 +0.4 320,503 + +0.1 320,533 + +0.7 320,533 + +1.1 320,816 + GFF4534 0.16 -1.7 320,850 + GFF4534 0.18 -1.0 320,929 + GFF4534 0.24 -0.3 320,946 + GFF4534 0.25 +3.0 320,947 - GFF4534 0.25 -1.0 321,177 + GFF4534 0.43 +0.4 321,204 + GFF4534 0.45 +0.3 321,204 + GFF4534 0.45 +0.3 321,322 - GFF4534 0.53 +0.3 321,552 + GFF4534 0.70 +0.1 321,653 - GFF4534 0.78 -0.8 321,653 - GFF4534 0.78 +0.1 321,661 + GFF4534 0.79 -1.4 321,662 - GFF4534 0.79 +0.7 321,690 + GFF4534 0.81 -2.6 321,691 - GFF4534 0.81 +0.3 321,714 + GFF4534 0.83 -2.2 321,850 - +0.5 322,373 - +0.9 322,531 + GFF4535 0.27 +1.5 322,531 + GFF4535 0.27 -0.6 322,664 - GFF4535 0.50 +0.7 322,838 + GFF4535 0.81 +0.2 322,852 + GFF4535 0.84 +0.7 322,897 - -0.0
Or see this region's nucleotide sequence