Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4366 and GFF4367 are separated by 212 nucleotides GFF4367 and GFF4368 are separated by 31 nucleotides GFF4368 and GFF4369 are separated by 99 nucleotides
GFF4366 - putative multidrug transporter membrane ATP-binding components, at 148,744 to 150,525
GFF4366
GFF4367 - Nitrogen regulatory protein P-II, glnK, at 150,738 to 151,076
GFF4367
GFF4368 - Ammonium transporter, at 151,108 to 152,394
GFF4368
GFF4369 - Acyl-CoA thioesterase II (EC 3.1.2.-), at 152,494 to 153,354
GFF4369
Position (kb)
151
152
153 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 150.145 kb on - strand, within GFF4366 at 150.234 kb on - strand, within GFF4366 at 150.615 kb on + strand at 150.616 kb on - strand at 150.616 kb on - strand at 150.717 kb on + strand at 150.737 kb on + strand at 150.738 kb on - strand at 150.738 kb on - strand at 150.888 kb on + strand, within GFF4367 at 150.982 kb on - strand, within GFF4367 at 150.982 kb on - strand, within GFF4367 at 151.341 kb on + strand, within GFF4368 at 151.342 kb on - strand, within GFF4368 at 151.459 kb on + strand, within GFF4368 at 151.467 kb on + strand, within GFF4368 at 151.592 kb on - strand, within GFF4368 at 151.592 kb on - strand, within GFF4368 at 151.592 kb on - strand, within GFF4368 at 151.666 kb on - strand, within GFF4368 at 151.716 kb on - strand, within GFF4368 at 151.716 kb on - strand, within GFF4368 at 151.929 kb on - strand, within GFF4368 at 151.929 kb on - strand, within GFF4368 at 152.005 kb on + strand, within GFF4368 at 152.079 kb on + strand, within GFF4368 at 152.080 kb on - strand, within GFF4368 at 152.114 kb on + strand, within GFF4368 at 152.248 kb on - strand, within GFF4368 at 152.254 kb on - strand, within GFF4368 at 152.384 kb on - strand at 152.386 kb on - strand at 152.424 kb on + strand at 152.439 kb on - strand at 152.559 kb on - strand at 152.614 kb on - strand, within GFF4369 at 152.615 kb on + strand, within GFF4369 at 152.616 kb on - strand, within GFF4369 at 152.616 kb on - strand, within GFF4369 at 152.616 kb on - strand, within GFF4369 at 152.658 kb on + strand, within GFF4369 at 152.658 kb on + strand, within GFF4369 at 152.659 kb on - strand, within GFF4369 at 152.659 kb on - strand, within GFF4369 at 152.659 kb on - strand, within GFF4369 at 153.100 kb on - strand, within GFF4369 at 153.354 kb on + strand at 153.354 kb on + strand at 153.355 kb on - strand at 153.358 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 150,145 - GFF4366 0.79 -0.1 150,234 - GFF4366 0.84 -2.1 150,615 + -0.3 150,616 - +0.8 150,616 - -0.5 150,717 + +0.6 150,737 + -1.2 150,738 - +1.1 150,738 - +1.9 150,888 + GFF4367 0.44 +0.0 150,982 - GFF4367 0.72 +0.7 150,982 - GFF4367 0.72 -0.1 151,341 + GFF4368 0.18 +1.2 151,342 - GFF4368 0.18 -0.2 151,459 + GFF4368 0.27 +0.2 151,467 + GFF4368 0.28 -4.8 151,592 - GFF4368 0.38 +0.4 151,592 - GFF4368 0.38 +0.6 151,592 - GFF4368 0.38 -0.3 151,666 - GFF4368 0.43 +0.6 151,716 - GFF4368 0.47 +0.7 151,716 - GFF4368 0.47 +0.0 151,929 - GFF4368 0.64 +1.5 151,929 - GFF4368 0.64 +1.5 152,005 + GFF4368 0.70 +0.1 152,079 + GFF4368 0.75 -0.4 152,080 - GFF4368 0.76 -1.4 152,114 + GFF4368 0.78 +1.4 152,248 - GFF4368 0.89 +1.4 152,254 - GFF4368 0.89 -2.9 152,384 - -2.6 152,386 - -0.8 152,424 + +0.8 152,439 - +0.4 152,559 - +0.1 152,614 - GFF4369 0.14 -3.2 152,615 + GFF4369 0.14 +0.6 152,616 - GFF4369 0.14 -0.0 152,616 - GFF4369 0.14 -0.9 152,616 - GFF4369 0.14 -0.8 152,658 + GFF4369 0.19 -0.6 152,658 + GFF4369 0.19 -1.3 152,659 - GFF4369 0.19 -1.9 152,659 - GFF4369 0.19 -2.7 152,659 - GFF4369 0.19 -0.4 153,100 - GFF4369 0.70 +1.1 153,354 + -0.6 153,354 + +0.3 153,355 - -1.7 153,358 + +0.2
Or see this region's nucleotide sequence