Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4325 and GFF4326 are separated by 0 nucleotides GFF4326 and GFF4327 are separated by 209 nucleotides GFF4327 and GFF4328 are separated by 43 nucleotides GFF4328 and GFF4329 are separated by 2 nucleotides
GFF4325 - Octaprenyl diphosphate synthase (EC 2.5.1.90) / Dimethylallyltransferase (EC 2.5.1.1) / (2E,6E)-farnesyl diphosphate synthase (EC 2.5.1.10) / Geranylgeranyl diphosphate synthase (EC 2.5.1.29), at 106,728 to 107,627
GFF4325
GFF4326 - Exodeoxyribonuclease VII small subunit (EC 3.1.11.6), at 107,628 to 107,870
GFF4326
GFF4327 - tRNA S(4)U 4-thiouridine synthase (former ThiI) / Rhodanese-like domain required for thiamine synthesis, at 108,080 to 109,528
GFF4327
GFF4328 - 2-aminoethylphosphonate ABC transporter permease protein II (TC 3.A.1.9.1), at 109,572 to 110,369
GFF4328
GFF4329 - 2-aminoethylphosphonate ABC transporter permease protein I (TC 3.A.1.9.1), at 110,372 to 111,232
GFF4329
Position (kb)
108
109
110 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 107.901 kb on - strand at 107.901 kb on - strand at 107.985 kb on + strand at 107.988 kb on - strand at 108.012 kb on - strand at 108.012 kb on - strand at 108.028 kb on - strand at 108.031 kb on - strand at 108.031 kb on - strand at 108.051 kb on - strand at 108.054 kb on + strand at 108.055 kb on - strand at 108.055 kb on - strand at 108.180 kb on + strand at 108.304 kb on + strand, within GFF4327 at 108.575 kb on + strand, within GFF4327 at 108.576 kb on - strand, within GFF4327 at 108.583 kb on + strand, within GFF4327 at 108.662 kb on + strand, within GFF4327 at 108.664 kb on + strand, within GFF4327 at 108.695 kb on + strand, within GFF4327 at 108.877 kb on + strand, within GFF4327 at 108.878 kb on - strand, within GFF4327 at 109.039 kb on + strand, within GFF4327 at 109.051 kb on + strand, within GFF4327 at 109.107 kb on - strand, within GFF4327 at 109.206 kb on + strand, within GFF4327 at 109.388 kb on + strand at 109.426 kb on + strand at 109.440 kb on + strand at 109.440 kb on + strand at 109.440 kb on + strand at 109.481 kb on + strand at 109.549 kb on + strand at 109.574 kb on - strand at 109.574 kb on - strand at 109.691 kb on + strand, within GFF4328 at 109.697 kb on + strand, within GFF4328 at 110.063 kb on + strand, within GFF4328 at 110.064 kb on - strand, within GFF4328 at 110.064 kb on - strand, within GFF4328 at 110.317 kb on - strand at 110.351 kb on + strand at 110.374 kb on - strand at 110.470 kb on + strand, within GFF4329 at 110.473 kb on - strand, within GFF4329 at 110.500 kb on - strand, within GFF4329
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 107,901 - -0.4 107,901 - -4.1 107,985 + -0.5 107,988 - -0.1 108,012 - -0.7 108,012 - +0.3 108,028 - +0.4 108,031 - -1.4 108,031 - -1.6 108,051 - -1.4 108,054 + +0.3 108,055 - -0.0 108,055 - -0.4 108,180 + -3.9 108,304 + GFF4327 0.15 -1.7 108,575 + GFF4327 0.34 +1.3 108,576 - GFF4327 0.34 -1.2 108,583 + GFF4327 0.35 -0.7 108,662 + GFF4327 0.40 -0.3 108,664 + GFF4327 0.40 +0.4 108,695 + GFF4327 0.42 +1.1 108,877 + GFF4327 0.55 -2.4 108,878 - GFF4327 0.55 +0.6 109,039 + GFF4327 0.66 +0.9 109,051 + GFF4327 0.67 -0.5 109,107 - GFF4327 0.71 -1.7 109,206 + GFF4327 0.78 -2.4 109,388 + -2.4 109,426 + +0.1 109,440 + -0.4 109,440 + -2.0 109,440 + -0.9 109,481 + -0.2 109,549 + -3.3 109,574 - -3.7 109,574 - -2.3 109,691 + GFF4328 0.15 +0.1 109,697 + GFF4328 0.16 -0.3 110,063 + GFF4328 0.62 +1.7 110,064 - GFF4328 0.62 +0.6 110,064 - GFF4328 0.62 +0.4 110,317 - +0.3 110,351 + +0.4 110,374 - -1.5 110,470 + GFF4329 0.11 +0.5 110,473 - GFF4329 0.12 +0.2 110,500 - GFF4329 0.15 -1.6
Or see this region's nucleotide sequence