Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4327

Experiment: 6H2_phage 10 MOI; agar plate assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4325 and GFF4326 are separated by 0 nucleotidesGFF4326 and GFF4327 are separated by 209 nucleotidesGFF4327 and GFF4328 are separated by 43 nucleotidesGFF4328 and GFF4329 are separated by 2 nucleotides GFF4325 - Octaprenyl diphosphate synthase (EC 2.5.1.90) / Dimethylallyltransferase (EC 2.5.1.1) / (2E,6E)-farnesyl diphosphate synthase (EC 2.5.1.10) / Geranylgeranyl diphosphate synthase (EC 2.5.1.29), at 106,728 to 107,627 GFF4325 GFF4326 - Exodeoxyribonuclease VII small subunit (EC 3.1.11.6), at 107,628 to 107,870 GFF4326 GFF4327 - tRNA S(4)U 4-thiouridine synthase (former ThiI) / Rhodanese-like domain required for thiamine synthesis, at 108,080 to 109,528 GFF4327 GFF4328 - 2-aminoethylphosphonate ABC transporter permease protein II (TC 3.A.1.9.1), at 109,572 to 110,369 GFF4328 GFF4329 - 2-aminoethylphosphonate ABC transporter permease protein I (TC 3.A.1.9.1), at 110,372 to 111,232 GFF4329 Position (kb) 108 109 110Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 107.901 kb on - strandat 107.901 kb on - strandat 107.985 kb on + strandat 107.988 kb on - strandat 108.012 kb on - strandat 108.012 kb on - strandat 108.028 kb on - strandat 108.031 kb on - strandat 108.031 kb on - strandat 108.051 kb on - strandat 108.054 kb on + strandat 108.055 kb on - strandat 108.055 kb on - strandat 108.180 kb on + strandat 108.304 kb on + strand, within GFF4327at 108.575 kb on + strand, within GFF4327at 108.576 kb on - strand, within GFF4327at 108.583 kb on + strand, within GFF4327at 108.662 kb on + strand, within GFF4327at 108.664 kb on + strand, within GFF4327at 108.695 kb on + strand, within GFF4327at 108.877 kb on + strand, within GFF4327at 108.878 kb on - strand, within GFF4327at 109.039 kb on + strand, within GFF4327at 109.051 kb on + strand, within GFF4327at 109.107 kb on - strand, within GFF4327at 109.206 kb on + strand, within GFF4327at 109.388 kb on + strandat 109.426 kb on + strandat 109.440 kb on + strandat 109.440 kb on + strandat 109.440 kb on + strandat 109.481 kb on + strandat 109.549 kb on + strandat 109.574 kb on - strandat 109.574 kb on - strandat 109.691 kb on + strand, within GFF4328at 109.697 kb on + strand, within GFF4328at 110.063 kb on + strand, within GFF4328at 110.064 kb on - strand, within GFF4328at 110.064 kb on - strand, within GFF4328at 110.317 kb on - strandat 110.351 kb on + strandat 110.374 kb on - strandat 110.470 kb on + strand, within GFF4329at 110.473 kb on - strand, within GFF4329at 110.500 kb on - strand, within GFF4329

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Per-strain Table

Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay
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107,901 - -0.4
107,901 - -4.1
107,985 + -0.5
107,988 - -0.1
108,012 - -0.7
108,012 - +0.3
108,028 - +0.4
108,031 - -1.4
108,031 - -1.6
108,051 - -1.4
108,054 + +0.3
108,055 - -0.0
108,055 - -0.4
108,180 + -3.9
108,304 + GFF4327 0.15 -1.7
108,575 + GFF4327 0.34 +1.3
108,576 - GFF4327 0.34 -1.2
108,583 + GFF4327 0.35 -0.7
108,662 + GFF4327 0.40 -0.3
108,664 + GFF4327 0.40 +0.4
108,695 + GFF4327 0.42 +1.1
108,877 + GFF4327 0.55 -2.4
108,878 - GFF4327 0.55 +0.6
109,039 + GFF4327 0.66 +0.9
109,051 + GFF4327 0.67 -0.5
109,107 - GFF4327 0.71 -1.7
109,206 + GFF4327 0.78 -2.4
109,388 + -2.4
109,426 + +0.1
109,440 + -0.4
109,440 + -2.0
109,440 + -0.9
109,481 + -0.2
109,549 + -3.3
109,574 - -3.7
109,574 - -2.3
109,691 + GFF4328 0.15 +0.1
109,697 + GFF4328 0.16 -0.3
110,063 + GFF4328 0.62 +1.7
110,064 - GFF4328 0.62 +0.6
110,064 - GFF4328 0.62 +0.4
110,317 - +0.3
110,351 + +0.4
110,374 - -1.5
110,470 + GFF4329 0.11 +0.5
110,473 - GFF4329 0.12 +0.2
110,500 - GFF4329 0.15 -1.6

Or see this region's nucleotide sequence