Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4292 and GFF4293 are separated by 172 nucleotides GFF4293 and GFF4294 overlap by 4 nucleotides
GFF4292 - Protein AraJ precursor, at 75,108 to 76,280
GFF4292
GFF4293 - Exonuclease SbcC, at 76,453 to 79,593
GFF4293
GFF4294 - Exonuclease SbcD, at 79,590 to 80,792
GFF4294
Position (kb) 76
77
78
79
80 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 75.496 kb on + strand, within GFF4292 at 75.531 kb on + strand, within GFF4292 at 75.531 kb on + strand, within GFF4292 at 75.561 kb on - strand, within GFF4292 at 75.599 kb on - strand, within GFF4292 at 75.605 kb on + strand, within GFF4292 at 75.606 kb on - strand, within GFF4292 at 75.606 kb on - strand, within GFF4292 at 75.606 kb on - strand, within GFF4292 at 75.619 kb on + strand, within GFF4292 at 76.025 kb on + strand, within GFF4292 at 76.025 kb on + strand, within GFF4292 at 76.073 kb on + strand, within GFF4292 at 76.074 kb on - strand, within GFF4292 at 76.079 kb on + strand, within GFF4292 at 76.141 kb on + strand, within GFF4292 at 76.155 kb on - strand, within GFF4292 at 76.350 kb on - strand at 76.371 kb on + strand at 76.406 kb on - strand at 76.406 kb on - strand at 76.413 kb on + strand at 76.413 kb on + strand at 76.413 kb on + strand at 76.413 kb on + strand at 76.413 kb on + strand at 76.413 kb on + strand at 76.427 kb on - strand at 76.455 kb on - strand at 76.516 kb on + strand at 76.920 kb on + strand, within GFF4293 at 76.920 kb on + strand, within GFF4293 at 76.921 kb on - strand, within GFF4293 at 76.921 kb on - strand, within GFF4293 at 76.955 kb on + strand, within GFF4293 at 77.131 kb on - strand, within GFF4293 at 77.317 kb on - strand, within GFF4293 at 77.635 kb on - strand, within GFF4293 at 77.862 kb on + strand, within GFF4293 at 77.936 kb on - strand, within GFF4293 at 77.936 kb on - strand, within GFF4293 at 77.936 kb on - strand, within GFF4293 at 78.095 kb on - strand, within GFF4293 at 78.186 kb on - strand, within GFF4293 at 78.478 kb on + strand, within GFF4293 at 78.794 kb on + strand, within GFF4293 at 78.795 kb on - strand, within GFF4293 at 78.940 kb on + strand, within GFF4293 at 78.986 kb on - strand, within GFF4293 at 79.201 kb on - strand, within GFF4293 at 79.207 kb on + strand, within GFF4293 at 79.208 kb on - strand, within GFF4293 at 79.722 kb on + strand, within GFF4294 at 79.785 kb on + strand, within GFF4294 at 79.788 kb on + strand, within GFF4294 at 79.788 kb on + strand, within GFF4294 at 79.789 kb on - strand, within GFF4294 at 80.193 kb on - strand, within GFF4294 at 80.194 kb on + strand, within GFF4294 at 80.194 kb on + strand, within GFF4294 at 80.238 kb on + strand, within GFF4294 at 80.381 kb on + strand, within GFF4294 at 80.451 kb on + strand, within GFF4294 at 80.584 kb on - strand, within GFF4294
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 75,496 + GFF4292 0.33 -0.4 75,531 + GFF4292 0.36 -2.6 75,531 + GFF4292 0.36 -0.6 75,561 - GFF4292 0.39 +0.6 75,599 - GFF4292 0.42 +0.0 75,605 + GFF4292 0.42 -0.4 75,606 - GFF4292 0.42 +1.8 75,606 - GFF4292 0.42 -2.6 75,606 - GFF4292 0.42 -0.4 75,619 + GFF4292 0.44 +1.4 76,025 + GFF4292 0.78 -1.4 76,025 + GFF4292 0.78 -0.3 76,073 + GFF4292 0.82 +0.0 76,074 - GFF4292 0.82 +1.0 76,079 + GFF4292 0.83 +1.2 76,141 + GFF4292 0.88 -1.5 76,155 - GFF4292 0.89 +0.0 76,350 - +0.7 76,371 + +0.4 76,406 - +0.3 76,406 - -1.5 76,413 + +0.4 76,413 + +0.4 76,413 + +0.1 76,413 + +0.2 76,413 + -2.0 76,413 + -0.3 76,427 - +0.8 76,455 - +0.5 76,516 + +0.2 76,920 + GFF4293 0.15 -2.4 76,920 + GFF4293 0.15 +0.4 76,921 - GFF4293 0.15 +0.4 76,921 - GFF4293 0.15 -1.4 76,955 + GFF4293 0.16 -1.3 77,131 - GFF4293 0.22 +0.6 77,317 - GFF4293 0.28 +0.5 77,635 - GFF4293 0.38 +0.0 77,862 + GFF4293 0.45 +0.2 77,936 - GFF4293 0.47 +0.7 77,936 - GFF4293 0.47 -1.5 77,936 - GFF4293 0.47 +0.3 78,095 - GFF4293 0.52 +0.0 78,186 - GFF4293 0.55 -1.5 78,478 + GFF4293 0.64 +0.0 78,794 + GFF4293 0.75 -0.7 78,795 - GFF4293 0.75 +0.4 78,940 + GFF4293 0.79 -3.1 78,986 - GFF4293 0.81 -0.4 79,201 - GFF4293 0.87 +1.1 79,207 + GFF4293 0.88 -0.2 79,208 - GFF4293 0.88 +0.2 79,722 + GFF4294 0.11 -0.4 79,785 + GFF4294 0.16 -1.6 79,788 + GFF4294 0.16 -0.9 79,788 + GFF4294 0.16 +0.6 79,789 - GFF4294 0.17 -0.6 80,193 - GFF4294 0.50 +2.4 80,194 + GFF4294 0.50 -0.0 80,194 + GFF4294 0.50 +0.3 80,238 + GFF4294 0.54 -1.3 80,381 + GFF4294 0.66 +1.1 80,451 + GFF4294 0.72 -0.8 80,584 - GFF4294 0.83 -0.9
Or see this region's nucleotide sequence