Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4112 and GFF4113 are separated by 65 nucleotides GFF4113 and GFF4114 are separated by 64 nucleotides GFF4114 and GFF4115 are separated by 11 nucleotides
GFF4112 - Outer membrane pore protein E precursor, at 70,390 to 71,442
GFF4112
GFF4113 - hypothetical protein, at 71,508 to 71,660
GFF4113
GFF4114 - Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA, at 71,725 to 72,828
GFF4114
GFF4115 - Gamma-glutamyl phosphate reductase (EC 1.2.1.41), at 72,840 to 74,090
GFF4115
Position (kb) 71
72
73 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 70.737 kb on - strand, within GFF4112 at 70.821 kb on + strand, within GFF4112 at 70.823 kb on + strand, within GFF4112 at 70.823 kb on + strand, within GFF4112 at 70.932 kb on - strand, within GFF4112 at 70.937 kb on - strand, within GFF4112 at 70.942 kb on - strand, within GFF4112 at 71.064 kb on + strand, within GFF4112 at 71.104 kb on - strand, within GFF4112 at 71.196 kb on + strand, within GFF4112 at 71.197 kb on - strand, within GFF4112 at 71.318 kb on + strand, within GFF4112 at 71.332 kb on - strand, within GFF4112 at 71.810 kb on + strand at 71.856 kb on + strand, within GFF4114 at 71.862 kb on + strand, within GFF4114 at 72.109 kb on - strand, within GFF4114 at 72.213 kb on + strand, within GFF4114 at 72.299 kb on - strand, within GFF4114 at 72.420 kb on + strand, within GFF4114 at 72.420 kb on + strand, within GFF4114 at 72.421 kb on - strand, within GFF4114 at 72.558 kb on + strand, within GFF4114 at 72.691 kb on - strand, within GFF4114 at 72.714 kb on + strand, within GFF4114 at 72.715 kb on - strand, within GFF4114 at 72.784 kb on + strand at 72.785 kb on - strand at 72.839 kb on + strand at 72.839 kb on + strand at 72.839 kb on + strand at 72.880 kb on - strand at 72.882 kb on - strand at 72.979 kb on + strand, within GFF4115 at 73.194 kb on + strand, within GFF4115 at 73.194 kb on + strand, within GFF4115 at 73.265 kb on - strand, within GFF4115 at 73.285 kb on - strand, within GFF4115 at 73.416 kb on + strand, within GFF4115 at 73.416 kb on + strand, within GFF4115 at 73.416 kb on + strand, within GFF4115 at 73.416 kb on + strand, within GFF4115 at 73.417 kb on - strand, within GFF4115 at 73.457 kb on + strand, within GFF4115 at 73.717 kb on + strand, within GFF4115
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 70,737 - GFF4112 0.33 -0.2 70,821 + GFF4112 0.41 -1.0 70,823 + GFF4112 0.41 -0.6 70,823 + GFF4112 0.41 -0.0 70,932 - GFF4112 0.51 -0.2 70,937 - GFF4112 0.52 +0.7 70,942 - GFF4112 0.52 -0.1 71,064 + GFF4112 0.64 +0.7 71,104 - GFF4112 0.68 +0.2 71,196 + GFF4112 0.77 +0.1 71,197 - GFF4112 0.77 +0.9 71,318 + GFF4112 0.88 +0.5 71,332 - GFF4112 0.89 +1.0 71,810 + +1.1 71,856 + GFF4114 0.12 +2.3 71,862 + GFF4114 0.12 +0.6 72,109 - GFF4114 0.35 +0.3 72,213 + GFF4114 0.44 +0.7 72,299 - GFF4114 0.52 +0.6 72,420 + GFF4114 0.63 +0.9 72,420 + GFF4114 0.63 +0.6 72,421 - GFF4114 0.63 -2.9 72,558 + GFF4114 0.75 -0.1 72,691 - GFF4114 0.88 -0.1 72,714 + GFF4114 0.90 -1.0 72,715 - GFF4114 0.90 -1.6 72,784 + +1.0 72,785 - -0.7 72,839 + +0.1 72,839 + -0.6 72,839 + +0.8 72,880 - -0.5 72,882 - -1.0 72,979 + GFF4115 0.11 -1.5 73,194 + GFF4115 0.28 +0.8 73,194 + GFF4115 0.28 +1.7 73,265 - GFF4115 0.34 -3.8 73,285 - GFF4115 0.36 -0.1 73,416 + GFF4115 0.46 -0.5 73,416 + GFF4115 0.46 +0.5 73,416 + GFF4115 0.46 -0.7 73,416 + GFF4115 0.46 +0.1 73,417 - GFF4115 0.46 +0.0 73,457 + GFF4115 0.49 -1.8 73,717 + GFF4115 0.70 -1.6
Or see this region's nucleotide sequence