Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4083 and GFF4084 overlap by 8 nucleotides GFF4084 and GFF4085 overlap by 50 nucleotides GFF4085 and GFF4086 are separated by 204 nucleotides GFF4086 and GFF4087 are separated by 202 nucleotides GFF4087 and GFF4088 are separated by 691 nucleotides GFF4088 and GFF4089 are separated by 0 nucleotides
GFF4083 - putative cytoplasmic protein, at 45,511 to 45,762
GFF4083
GFF4084 - FIG01045272: hypothetical protein, at 45,755 to 45,913
GFF4084
GFF4085 - FIG01046190: hypothetical protein, at 45,864 to 46,040
GFF4085
GFF4086 - hypothetical protein, at 46,245 to 46,385
GFF4086
GFF4087 - Mobile element protein, at 46,588 to 46,869
GFF4087
GFF4088 - hypothetical protein, at 47,561 to 47,686
GFF4088
GFF4089 - hypothetical protein, at 47,687 to 47,818
GFF4089
Position (kb) 46
47 Strain fitness (log2 ratio) -2
-1
0
1 at 46.084 kb on + strand at 46.085 kb on - strand at 46.088 kb on + strand at 46.089 kb on - strand at 46.121 kb on - strand at 46.121 kb on - strand at 46.137 kb on - strand at 46.210 kb on - strand at 46.236 kb on + strand at 46.312 kb on - strand, within GFF4086 at 46.740 kb on - strand, within GFF4087 at 47.073 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 46,084 + -1.2 46,085 - +0.8 46,088 + -1.1 46,089 - +1.7 46,121 - +0.4 46,121 - -0.4 46,137 - -0.0 46,210 - -2.5 46,236 + +0.7 46,312 - GFF4086 0.48 +0.5 46,740 - GFF4087 0.54 +0.3 47,073 - -0.1
Or see this region's nucleotide sequence