Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF404 and GFF405 are separated by 73 nucleotides GFF405 and GFF406 are separated by 267 nucleotides
GFF404 - D-glucarate permease, at 119,043 to 120,353
GFF404
GFF405 - L-talarate dehydratase @ Galactarate dehydratase (EC 4.2.1.42), at 120,427 to 121,623
GFF405
GFF406 - Transcriptional regulator, LacI family, at 121,891 to 122,946
GFF406
Position (kb)
120
121
122 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 119.433 kb on - strand, within GFF404 at 119.560 kb on + strand, within GFF404 at 119.595 kb on - strand, within GFF404 at 119.693 kb on + strand, within GFF404 at 120.052 kb on + strand, within GFF404 at 120.053 kb on - strand, within GFF404 at 120.058 kb on + strand, within GFF404 at 120.058 kb on + strand, within GFF404 at 120.172 kb on + strand, within GFF404 at 120.172 kb on + strand, within GFF404 at 120.240 kb on + strand at 120.342 kb on + strand at 120.358 kb on + strand at 120.654 kb on + strand, within GFF405 at 120.654 kb on + strand, within GFF405 at 120.734 kb on - strand, within GFF405 at 120.811 kb on - strand, within GFF405 at 120.860 kb on - strand, within GFF405 at 120.880 kb on - strand, within GFF405 at 120.880 kb on - strand, within GFF405 at 120.880 kb on - strand, within GFF405 at 121.005 kb on + strand, within GFF405 at 121.104 kb on + strand, within GFF405 at 121.192 kb on - strand, within GFF405 at 121.230 kb on + strand, within GFF405 at 121.231 kb on - strand, within GFF405 at 121.291 kb on + strand, within GFF405 at 121.325 kb on - strand, within GFF405 at 121.337 kb on - strand, within GFF405 at 121.355 kb on + strand, within GFF405 at 121.356 kb on - strand, within GFF405 at 121.356 kb on - strand, within GFF405 at 121.360 kb on - strand, within GFF405 at 121.386 kb on - strand, within GFF405 at 121.478 kb on - strand, within GFF405 at 121.525 kb on + strand at 121.573 kb on - strand at 121.589 kb on + strand at 121.615 kb on + strand at 121.725 kb on + strand at 121.725 kb on + strand at 121.725 kb on + strand at 121.725 kb on + strand at 121.725 kb on + strand at 121.725 kb on + strand at 121.725 kb on + strand at 121.726 kb on - strand at 121.726 kb on - strand at 121.726 kb on - strand at 121.726 kb on - strand at 121.726 kb on - strand at 121.726 kb on - strand at 121.726 kb on - strand at 121.735 kb on - strand at 121.848 kb on - strand at 121.880 kb on + strand at 121.881 kb on - strand at 122.099 kb on - strand, within GFF406 at 122.148 kb on - strand, within GFF406 at 122.241 kb on - strand, within GFF406 at 122.260 kb on + strand, within GFF406 at 122.348 kb on + strand, within GFF406 at 122.473 kb on + strand, within GFF406 at 122.474 kb on - strand, within GFF406 at 122.553 kb on - strand, within GFF406 at 122.553 kb on - strand, within GFF406 at 122.556 kb on + strand, within GFF406 at 122.556 kb on + strand, within GFF406 at 122.557 kb on - strand, within GFF406
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 119,433 - GFF404 0.30 +0.2 119,560 + GFF404 0.39 -2.1 119,595 - GFF404 0.42 +0.6 119,693 + GFF404 0.50 -2.2 120,052 + GFF404 0.77 +0.2 120,053 - GFF404 0.77 -4.3 120,058 + GFF404 0.77 -1.2 120,058 + GFF404 0.77 -0.6 120,172 + GFF404 0.86 +0.4 120,172 + GFF404 0.86 +0.9 120,240 + -3.8 120,342 + +1.2 120,358 + +0.4 120,654 + GFF405 0.19 -3.2 120,654 + GFF405 0.19 +0.3 120,734 - GFF405 0.26 -1.5 120,811 - GFF405 0.32 -0.7 120,860 - GFF405 0.36 -1.2 120,880 - GFF405 0.38 -0.4 120,880 - GFF405 0.38 +0.3 120,880 - GFF405 0.38 -0.1 121,005 + GFF405 0.48 +0.6 121,104 + GFF405 0.57 +1.7 121,192 - GFF405 0.64 -1.4 121,230 + GFF405 0.67 +0.0 121,231 - GFF405 0.67 +0.0 121,291 + GFF405 0.72 -0.6 121,325 - GFF405 0.75 -1.8 121,337 - GFF405 0.76 +0.3 121,355 + GFF405 0.78 +0.3 121,356 - GFF405 0.78 +1.6 121,356 - GFF405 0.78 +0.3 121,360 - GFF405 0.78 -0.7 121,386 - GFF405 0.80 +0.3 121,478 - GFF405 0.88 +0.2 121,525 + +0.2 121,573 - +0.8 121,589 + +1.2 121,615 + +0.3 121,725 + +0.5 121,725 + +0.0 121,725 + +0.2 121,725 + +0.5 121,725 + +0.8 121,725 + -0.2 121,725 + -0.2 121,726 - +1.3 121,726 - +0.7 121,726 - -0.3 121,726 - +0.2 121,726 - +0.5 121,726 - +0.2 121,726 - -0.2 121,735 - -2.6 121,848 - -4.2 121,880 + -2.2 121,881 - -0.9 122,099 - GFF406 0.20 +1.7 122,148 - GFF406 0.24 +0.8 122,241 - GFF406 0.33 -0.5 122,260 + GFF406 0.35 -0.0 122,348 + GFF406 0.43 +0.6 122,473 + GFF406 0.55 +0.9 122,474 - GFF406 0.55 +0.6 122,553 - GFF406 0.63 -0.1 122,553 - GFF406 0.63 -0.7 122,556 + GFF406 0.63 -0.3 122,556 + GFF406 0.63 +0.4 122,557 - GFF406 0.63 -0.1
Or see this region's nucleotide sequence