Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3808 and GFF3809 are separated by 48 nucleotides GFF3809 and GFF3810 are separated by 158 nucleotides GFF3810 and GFF3811 are separated by 204 nucleotides
GFF3808 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 76,068 to 76,850
GFF3808
GFF3809 - DedA family inner membrane protein YohD, at 76,899 to 77,468
GFF3809
GFF3810 - Putative membrane protein, at 77,627 to 78,214
GFF3810
GFF3811 - Murein-DD-endopeptidase (EC 3.4.99.-), at 78,419 to 79,327
GFF3811
Position (kb)
77
78
79 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 76.727 kb on + strand, within GFF3808 at 76.727 kb on + strand, within GFF3808 at 76.728 kb on - strand, within GFF3808 at 76.779 kb on + strand at 76.779 kb on + strand at 76.779 kb on + strand at 77.165 kb on + strand, within GFF3809 at 77.191 kb on + strand, within GFF3809 at 77.235 kb on - strand, within GFF3809 at 77.272 kb on + strand, within GFF3809 at 77.290 kb on + strand, within GFF3809 at 77.291 kb on - strand, within GFF3809 at 77.440 kb on + strand at 77.440 kb on + strand at 77.450 kb on + strand at 77.472 kb on + strand at 77.473 kb on - strand at 77.495 kb on + strand at 77.495 kb on + strand at 77.495 kb on + strand at 77.496 kb on - strand at 77.496 kb on - strand at 77.496 kb on - strand at 77.496 kb on - strand at 77.546 kb on - strand at 77.637 kb on + strand at 77.637 kb on + strand at 77.637 kb on + strand at 77.637 kb on + strand at 77.638 kb on - strand at 77.713 kb on + strand, within GFF3810 at 77.713 kb on + strand, within GFF3810 at 77.714 kb on - strand, within GFF3810 at 77.714 kb on - strand, within GFF3810 at 77.808 kb on + strand, within GFF3810 at 77.809 kb on - strand, within GFF3810 at 77.809 kb on - strand, within GFF3810 at 77.852 kb on + strand, within GFF3810 at 78.012 kb on + strand, within GFF3810 at 78.013 kb on - strand, within GFF3810 at 78.051 kb on - strand, within GFF3810 at 78.318 kb on + strand at 78.320 kb on + strand at 78.321 kb on - strand at 78.321 kb on - strand at 78.684 kb on - strand, within GFF3811 at 78.724 kb on + strand, within GFF3811 at 78.809 kb on - strand, within GFF3811 at 78.854 kb on - strand, within GFF3811 at 78.960 kb on + strand, within GFF3811 at 78.961 kb on - strand, within GFF3811 at 79.041 kb on - strand, within GFF3811 at 79.169 kb on + strand, within GFF3811 at 79.172 kb on - strand, within GFF3811 at 79.201 kb on + strand, within GFF3811 at 79.202 kb on - strand, within GFF3811
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 76,727 + GFF3808 0.84 -1.0 76,727 + GFF3808 0.84 +0.0 76,728 - GFF3808 0.84 -2.3 76,779 + -1.3 76,779 + +1.3 76,779 + +0.6 77,165 + GFF3809 0.47 +0.7 77,191 + GFF3809 0.51 -0.3 77,235 - GFF3809 0.59 +1.0 77,272 + GFF3809 0.65 -3.8 77,290 + GFF3809 0.69 -1.7 77,291 - GFF3809 0.69 -0.2 77,440 + +0.3 77,440 + -2.1 77,450 + +0.2 77,472 + +1.0 77,473 - -1.3 77,495 + -3.0 77,495 + -2.7 77,495 + +0.5 77,496 - +0.8 77,496 - -0.1 77,496 - +0.3 77,496 - +0.2 77,546 - +1.5 77,637 + -2.4 77,637 + -0.2 77,637 + -0.8 77,637 + +0.3 77,638 - -0.9 77,713 + GFF3810 0.15 -3.1 77,713 + GFF3810 0.15 +0.5 77,714 - GFF3810 0.15 -1.0 77,714 - GFF3810 0.15 -1.4 77,808 + GFF3810 0.31 -1.8 77,809 - GFF3810 0.31 -1.7 77,809 - GFF3810 0.31 -1.8 77,852 + GFF3810 0.38 -1.0 78,012 + GFF3810 0.65 -3.0 78,013 - GFF3810 0.66 +0.4 78,051 - GFF3810 0.72 -0.0 78,318 + +1.3 78,320 + -0.2 78,321 - -0.1 78,321 - +1.1 78,684 - GFF3811 0.29 -0.6 78,724 + GFF3811 0.34 +0.2 78,809 - GFF3811 0.43 -3.3 78,854 - GFF3811 0.48 -0.2 78,960 + GFF3811 0.60 -0.3 78,961 - GFF3811 0.60 +0.7 79,041 - GFF3811 0.68 +1.5 79,169 + GFF3811 0.83 +3.3 79,172 - GFF3811 0.83 -0.6 79,201 + GFF3811 0.86 -0.1 79,202 - GFF3811 0.86 +0.1
Or see this region's nucleotide sequence