Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3580 and GFF3581 are separated by 178 nucleotides GFF3581 and GFF3582 are separated by 30 nucleotides GFF3582 and GFF3583 are separated by 418 nucleotides GFF3583 and GFF3584 are separated by 1 nucleotides
GFF3580 - Transcriptional regulator, MerR family, associated with photolyase, at 337,313 to 338,041
GFF3580
GFF3581 - Secretion system regulator of DegU/UvrY/BvgA type, at 338,220 to 338,858
GFF3581
GFF3582 - Secretion system regulator: Sensor component (EC 2.7.3.-) (EC 2.7.1.40), at 338,889 to 341,651
GFF3582
GFF3583 - Type III secretion protein SsaB, at 342,070 to 342,453
GFF3583
GFF3584 - Type III secretion outermembrane pore forming protein (YscC,MxiD,HrcC, InvG), at 342,455 to 343,948
GFF3584
Position (kb) 338
339
340
341
342 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 337.922 kb on + strand, within GFF3580 at 337.923 kb on - strand, within GFF3580 at 337.943 kb on - strand, within GFF3580 at 338.010 kb on - strand at 338.073 kb on - strand at 338.075 kb on - strand at 338.075 kb on - strand at 338.094 kb on + strand at 338.105 kb on + strand at 338.199 kb on - strand at 338.589 kb on - strand, within GFF3581 at 338.590 kb on + strand, within GFF3581 at 338.590 kb on + strand, within GFF3581 at 338.603 kb on - strand, within GFF3581 at 338.646 kb on + strand, within GFF3581 at 338.688 kb on - strand, within GFF3581 at 338.704 kb on - strand, within GFF3581 at 338.704 kb on - strand, within GFF3581 at 338.791 kb on - strand, within GFF3581 at 339.149 kb on + strand at 339.733 kb on - strand, within GFF3582 at 340.056 kb on + strand, within GFF3582 at 340.257 kb on - strand, within GFF3582 at 340.313 kb on + strand, within GFF3582 at 340.707 kb on + strand, within GFF3582 at 340.711 kb on - strand, within GFF3582 at 341.149 kb on - strand, within GFF3582 at 341.943 kb on - strand at 342.258 kb on - strand, within GFF3583
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 337,922 + GFF3580 0.84 +0.1 337,923 - GFF3580 0.84 +2.6 337,943 - GFF3580 0.86 +0.9 338,010 - -3.9 338,073 - +1.2 338,075 - +0.4 338,075 - +1.0 338,094 + +0.5 338,105 + +1.1 338,199 - +0.4 338,589 - GFF3581 0.58 +0.7 338,590 + GFF3581 0.58 +0.2 338,590 + GFF3581 0.58 -1.8 338,603 - GFF3581 0.60 +0.4 338,646 + GFF3581 0.67 -1.6 338,688 - GFF3581 0.73 +0.7 338,704 - GFF3581 0.76 +0.6 338,704 - GFF3581 0.76 -2.6 338,791 - GFF3581 0.89 +0.2 339,149 + -3.0 339,733 - GFF3582 0.31 -3.3 340,056 + GFF3582 0.42 -1.1 340,257 - GFF3582 0.50 -0.0 340,313 + GFF3582 0.52 +1.8 340,707 + GFF3582 0.66 +1.3 340,711 - GFF3582 0.66 -0.2 341,149 - GFF3582 0.82 -2.5 341,943 - +0.9 342,258 - GFF3583 0.49 -0.7
Or see this region's nucleotide sequence