Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3324 and GFF3325 are separated by 88 nucleotides GFF3325 and GFF3326 are separated by 118 nucleotides GFF3326 and GFF3327 are separated by 16 nucleotides
GFF3324 - putative periplasmic protein, at 94,655 to 94,975
GFF3324
GFF3325 - COG2110, Macro domain, possibly ADP-ribose binding module, at 95,064 to 95,603
GFF3325
GFF3326 - Cardiolipin synthetase (EC 2.7.8.-), at 95,722 to 97,026
GFF3326
GFF3327 - Glucans biosynthesis protein C (EC 2.1.-.-), at 97,043 to 98,197
GFF3327
Position (kb) 95
96
97
98 Strain fitness (log2 ratio) -2
-1
0
1
2 at 94.961 kb on - strand at 94.988 kb on - strand at 95.087 kb on - strand at 95.096 kb on + strand at 95.297 kb on + strand, within GFF3325 at 95.439 kb on + strand, within GFF3325 at 95.506 kb on - strand, within GFF3325 at 95.524 kb on + strand, within GFF3325 at 95.539 kb on - strand, within GFF3325 at 95.572 kb on + strand at 95.572 kb on + strand at 95.752 kb on + strand at 95.755 kb on - strand at 95.770 kb on - strand at 95.847 kb on + strand at 95.899 kb on - strand, within GFF3326 at 95.901 kb on + strand, within GFF3326 at 96.188 kb on + strand, within GFF3326 at 96.188 kb on + strand, within GFF3326 at 96.188 kb on + strand, within GFF3326 at 96.189 kb on - strand, within GFF3326 at 96.189 kb on - strand, within GFF3326 at 96.245 kb on - strand, within GFF3326 at 96.246 kb on + strand, within GFF3326 at 96.276 kb on + strand, within GFF3326 at 96.277 kb on - strand, within GFF3326 at 96.369 kb on + strand, within GFF3326 at 96.447 kb on + strand, within GFF3326 at 96.505 kb on - strand, within GFF3326 at 96.539 kb on + strand, within GFF3326 at 96.540 kb on - strand, within GFF3326 at 96.544 kb on - strand, within GFF3326 at 96.668 kb on + strand, within GFF3326 at 96.669 kb on - strand, within GFF3326 at 96.708 kb on - strand, within GFF3326 at 96.714 kb on + strand, within GFF3326 at 96.736 kb on - strand, within GFF3326 at 96.907 kb on - strand at 97.119 kb on - strand at 97.119 kb on - strand at 97.160 kb on + strand, within GFF3327 at 97.252 kb on - strand, within GFF3327 at 97.408 kb on - strand, within GFF3327 at 97.426 kb on - strand, within GFF3327 at 97.599 kb on - strand, within GFF3327 at 97.630 kb on - strand, within GFF3327 at 97.726 kb on + strand, within GFF3327 at 97.727 kb on - strand, within GFF3327
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 94,961 - -0.3 94,988 - -1.8 95,087 - -0.3 95,096 + -0.6 95,297 + GFF3325 0.43 -0.0 95,439 + GFF3325 0.69 -1.7 95,506 - GFF3325 0.82 -0.0 95,524 + GFF3325 0.85 -0.8 95,539 - GFF3325 0.88 -0.5 95,572 + +1.1 95,572 + +2.3 95,752 + +1.4 95,755 - -0.7 95,770 - -0.5 95,847 + -2.3 95,899 - GFF3326 0.14 +0.8 95,901 + GFF3326 0.14 +0.1 96,188 + GFF3326 0.36 +0.4 96,188 + GFF3326 0.36 +0.8 96,188 + GFF3326 0.36 -0.4 96,189 - GFF3326 0.36 +2.2 96,189 - GFF3326 0.36 -0.6 96,245 - GFF3326 0.40 +0.9 96,246 + GFF3326 0.40 -0.5 96,276 + GFF3326 0.42 +1.3 96,277 - GFF3326 0.43 +1.5 96,369 + GFF3326 0.50 -1.3 96,447 + GFF3326 0.56 -0.1 96,505 - GFF3326 0.60 -0.8 96,539 + GFF3326 0.63 +1.0 96,540 - GFF3326 0.63 +0.5 96,544 - GFF3326 0.63 +0.1 96,668 + GFF3326 0.72 -2.5 96,669 - GFF3326 0.73 -2.3 96,708 - GFF3326 0.76 -0.4 96,714 + GFF3326 0.76 +1.2 96,736 - GFF3326 0.78 +1.0 96,907 - -2.4 97,119 - +0.0 97,119 - +1.2 97,160 + GFF3327 0.10 +0.2 97,252 - GFF3327 0.18 +0.5 97,408 - GFF3327 0.32 -0.7 97,426 - GFF3327 0.33 +1.1 97,599 - GFF3327 0.48 +0.5 97,630 - GFF3327 0.51 -0.2 97,726 + GFF3327 0.59 -1.5 97,727 - GFF3327 0.59 +0.2
Or see this region's nucleotide sequence