Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3301 and GFF3302 are separated by 60 nucleotides GFF3302 and GFF3303 are separated by 229 nucleotides
GFF3301 - Transcriptional repressor of PutA and PutP / Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase) / Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.2.1.88), at 69,463 to 73,425
GFF3301
GFF3302 - FIG00639374: hypothetical protein, at 73,486 to 73,617
GFF3302
GFF3303 - Proline/sodium symporter PutP (TC 2.A.21.2.1) @ Propionate/sodium symporter, at 73,847 to 75,355
GFF3303
Position (kb)
73
74 Strain fitness (log2 ratio)
-2
-1
0
1 at 72.576 kb on - strand, within GFF3301 at 72.809 kb on - strand, within GFF3301 at 72.809 kb on - strand, within GFF3301 at 72.874 kb on + strand, within GFF3301 at 72.889 kb on - strand, within GFF3301 at 73.268 kb on - strand at 73.287 kb on + strand at 73.307 kb on + strand at 73.308 kb on - strand at 73.311 kb on + strand at 73.615 kb on + strand at 73.769 kb on + strand at 73.769 kb on + strand at 73.769 kb on + strand at 73.769 kb on + strand at 73.769 kb on + strand at 73.769 kb on + strand at 73.770 kb on - strand at 73.852 kb on + strand at 73.856 kb on - strand at 73.949 kb on + strand at 74.011 kb on + strand, within GFF3303 at 74.161 kb on + strand, within GFF3303 at 74.206 kb on + strand, within GFF3303 at 74.207 kb on - strand, within GFF3303 at 74.207 kb on - strand, within GFF3303 at 74.260 kb on + strand, within GFF3303 at 74.264 kb on + strand, within GFF3303 at 74.264 kb on + strand, within GFF3303 at 74.265 kb on - strand, within GFF3303 at 74.461 kb on + strand, within GFF3303 at 74.588 kb on + strand, within GFF3303 at 74.588 kb on + strand, within GFF3303 at 74.606 kb on - strand, within GFF3303
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 72,576 - GFF3301 0.79 +0.5 72,809 - GFF3301 0.84 -2.4 72,809 - GFF3301 0.84 +1.3 72,874 + GFF3301 0.86 -0.3 72,889 - GFF3301 0.86 -0.3 73,268 - -0.8 73,287 + -0.1 73,307 + -0.1 73,308 - +1.5 73,311 + -1.3 73,615 + +1.1 73,769 + +0.9 73,769 + +1.5 73,769 + +0.1 73,769 + +0.9 73,769 + -0.4 73,769 + +1.1 73,770 - -1.7 73,852 + +0.6 73,856 - +1.0 73,949 + +0.3 74,011 + GFF3303 0.11 +0.7 74,161 + GFF3303 0.21 -0.0 74,206 + GFF3303 0.24 +1.1 74,207 - GFF3303 0.24 -2.4 74,207 - GFF3303 0.24 -1.5 74,260 + GFF3303 0.27 +0.1 74,264 + GFF3303 0.28 +0.9 74,264 + GFF3303 0.28 +0.0 74,265 - GFF3303 0.28 -2.2 74,461 + GFF3303 0.41 -0.3 74,588 + GFF3303 0.49 +1.2 74,588 + GFF3303 0.49 -1.2 74,606 - GFF3303 0.50 -0.2
Or see this region's nucleotide sequence