Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3209 and GFF3210 are separated by 20 nucleotides GFF3210 and GFF3211 are separated by 97 nucleotides
GFF3209 - L-threonine transporter, anaerobically inducible, at 215,169 to 216,500
GFF3209
GFF3210 - Threonine dehydratase, catabolic (EC 4.3.1.19), at 216,521 to 217,510
GFF3210
GFF3211 - Threonine catabolic operon transcriptional activator TdcA, at 217,608 to 218,546
GFF3211
Position (kb) 216
217
218 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 215.551 kb on + strand, within GFF3209 at 215.684 kb on + strand, within GFF3209 at 215.721 kb on - strand, within GFF3209 at 215.721 kb on - strand, within GFF3209 at 215.721 kb on - strand, within GFF3209 at 215.750 kb on + strand, within GFF3209 at 215.786 kb on - strand, within GFF3209 at 215.799 kb on - strand, within GFF3209 at 215.997 kb on + strand, within GFF3209 at 216.004 kb on + strand, within GFF3209 at 216.141 kb on - strand, within GFF3209 at 216.144 kb on - strand, within GFF3209 at 216.158 kb on - strand, within GFF3209 at 216.190 kb on + strand, within GFF3209 at 216.208 kb on - strand, within GFF3209 at 216.248 kb on + strand, within GFF3209 at 216.307 kb on + strand, within GFF3209 at 216.307 kb on + strand, within GFF3209 at 216.308 kb on - strand, within GFF3209 at 216.322 kb on + strand, within GFF3209 at 216.478 kb on - strand at 216.486 kb on - strand at 216.492 kb on - strand at 216.673 kb on + strand, within GFF3210 at 217.313 kb on - strand, within GFF3210 at 217.313 kb on - strand, within GFF3210 at 217.435 kb on - strand at 217.845 kb on - strand, within GFF3211 at 217.846 kb on + strand, within GFF3211 at 217.930 kb on + strand, within GFF3211 at 218.067 kb on + strand, within GFF3211 at 218.195 kb on + strand, within GFF3211 at 218.196 kb on - strand, within GFF3211 at 218.276 kb on + strand, within GFF3211 at 218.340 kb on + strand, within GFF3211 at 218.341 kb on - strand, within GFF3211
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 215,551 + GFF3209 0.29 -2.8 215,684 + GFF3209 0.39 +0.3 215,721 - GFF3209 0.41 +0.6 215,721 - GFF3209 0.41 +0.6 215,721 - GFF3209 0.41 -2.5 215,750 + GFF3209 0.44 +0.4 215,786 - GFF3209 0.46 +0.5 215,799 - GFF3209 0.47 -0.2 215,997 + GFF3209 0.62 -1.9 216,004 + GFF3209 0.63 -1.0 216,141 - GFF3209 0.73 -0.0 216,144 - GFF3209 0.73 +0.6 216,158 - GFF3209 0.74 -0.6 216,190 + GFF3209 0.77 +1.4 216,208 - GFF3209 0.78 -0.1 216,248 + GFF3209 0.81 +1.0 216,307 + GFF3209 0.85 +0.7 216,307 + GFF3209 0.85 -0.8 216,308 - GFF3209 0.86 -0.5 216,322 + GFF3209 0.87 +0.5 216,478 - -0.1 216,486 - -3.6 216,492 - +0.6 216,673 + GFF3210 0.15 +1.5 217,313 - GFF3210 0.80 +2.9 217,313 - GFF3210 0.80 -1.1 217,435 - +0.4 217,845 - GFF3211 0.25 -0.2 217,846 + GFF3211 0.25 +0.2 217,930 + GFF3211 0.34 -0.9 218,067 + GFF3211 0.49 -0.5 218,195 + GFF3211 0.63 +0.4 218,196 - GFF3211 0.63 -3.5 218,276 + GFF3211 0.71 -1.6 218,340 + GFF3211 0.78 +0.8 218,341 - GFF3211 0.78 +1.4
Or see this region's nucleotide sequence