Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3114 and GFF3115 overlap by 14 nucleotides GFF3115 and GFF3116 are separated by 123 nucleotides GFF3116 and GFF3117 are separated by 112 nucleotides GFF3117 and GFF3118 are separated by 6 nucleotides
GFF3114 - FIG01045310: hypothetical protein, at 122,790 to 123,200
GFF3114
GFF3115 - putative cytoplasmic protein, at 123,187 to 123,591
GFF3115
GFF3116 - FIG00553873: hypothetical protein, at 123,715 to 124,599
GFF3116
GFF3117 - Biopolymer transport protein ExbD/TolR, at 124,712 to 125,137
GFF3117
GFF3118 - MotA/TolQ/ExbB proton channel family protein, at 125,144 to 125,878
GFF3118
Position (kb) 123
124
125 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 123.376 kb on + strand, within GFF3115 at 123.376 kb on + strand, within GFF3115 at 123.376 kb on + strand, within GFF3115 at 123.376 kb on + strand, within GFF3115 at 123.376 kb on + strand, within GFF3115 at 123.376 kb on + strand, within GFF3115 at 123.377 kb on - strand, within GFF3115 at 123.377 kb on - strand, within GFF3115 at 123.515 kb on + strand, within GFF3115 at 123.515 kb on + strand, within GFF3115 at 123.516 kb on - strand, within GFF3115 at 123.602 kb on + strand at 123.606 kb on + strand at 123.725 kb on + strand at 123.726 kb on - strand at 124.075 kb on - strand, within GFF3116 at 124.149 kb on - strand, within GFF3116 at 124.272 kb on + strand, within GFF3116 at 124.339 kb on + strand, within GFF3116 at 124.340 kb on - strand, within GFF3116 at 124.519 kb on + strand at 124.520 kb on - strand at 124.520 kb on - strand at 124.520 kb on - strand at 124.525 kb on + strand at 124.525 kb on + strand at 124.526 kb on - strand at 124.549 kb on + strand at 124.550 kb on - strand at 124.593 kb on - strand at 124.615 kb on - strand at 124.869 kb on - strand, within GFF3117 at 124.912 kb on + strand, within GFF3117 at 124.945 kb on - strand, within GFF3117 at 124.981 kb on + strand, within GFF3117 at 125.175 kb on - strand at 125.232 kb on - strand, within GFF3118 at 125.265 kb on + strand, within GFF3118 at 125.266 kb on - strand, within GFF3118 at 125.359 kb on + strand, within GFF3118 at 125.423 kb on - strand, within GFF3118 at 125.570 kb on - strand, within GFF3118 at 125.570 kb on - strand, within GFF3118
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 123,376 + GFF3115 0.47 -1.9 123,376 + GFF3115 0.47 +0.3 123,376 + GFF3115 0.47 +0.3 123,376 + GFF3115 0.47 +0.3 123,376 + GFF3115 0.47 -0.3 123,376 + GFF3115 0.47 +0.1 123,377 - GFF3115 0.47 -3.0 123,377 - GFF3115 0.47 +0.5 123,515 + GFF3115 0.81 +0.4 123,515 + GFF3115 0.81 -1.9 123,516 - GFF3115 0.81 -0.4 123,602 + -0.5 123,606 + +0.8 123,725 + -0.3 123,726 - +0.2 124,075 - GFF3116 0.41 +0.5 124,149 - GFF3116 0.49 +0.2 124,272 + GFF3116 0.63 +1.5 124,339 + GFF3116 0.71 -0.5 124,340 - GFF3116 0.71 +1.4 124,519 + +2.2 124,520 - -1.0 124,520 - +0.8 124,520 - +0.2 124,525 + +0.8 124,525 + -2.3 124,526 - +0.5 124,549 + -0.0 124,550 - +0.4 124,593 - +2.1 124,615 - -1.0 124,869 - GFF3117 0.37 +0.1 124,912 + GFF3117 0.47 -3.2 124,945 - GFF3117 0.55 -1.0 124,981 + GFF3117 0.63 -2.6 125,175 - -0.4 125,232 - GFF3118 0.12 +0.5 125,265 + GFF3118 0.16 -0.9 125,266 - GFF3118 0.17 +0.0 125,359 + GFF3118 0.29 -0.2 125,423 - GFF3118 0.38 -3.4 125,570 - GFF3118 0.58 -1.8 125,570 - GFF3118 0.58 -0.5
Or see this region's nucleotide sequence