Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2820 and GFF2821 are separated by 204 nucleotides GFF2821 and GFF2822 are separated by 71 nucleotides GFF2822 and GFF2823 are separated by 57 nucleotides
GFF2820 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 340,491 to 342,176
GFF2820
GFF2821 - Starvation lipoprotein Slp paralog, at 342,381 to 342,962
GFF2821
GFF2822 - TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA, at 343,034 to 343,729
GFF2822
GFF2823 - DinG family ATP-dependent helicase YoaA, at 343,787 to 345,697
GFF2823
Position (kb) 342
343 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 341.486 kb on + strand, within GFF2820 at 341.537 kb on + strand, within GFF2820 at 341.539 kb on + strand, within GFF2820 at 341.694 kb on - strand, within GFF2820 at 341.736 kb on - strand, within GFF2820 at 341.764 kb on - strand, within GFF2820 at 341.789 kb on + strand, within GFF2820 at 341.905 kb on - strand, within GFF2820 at 342.033 kb on + strand at 342.424 kb on + strand at 342.424 kb on + strand at 342.561 kb on - strand, within GFF2821 at 342.563 kb on - strand, within GFF2821 at 342.621 kb on + strand, within GFF2821 at 342.622 kb on - strand, within GFF2821 at 342.622 kb on - strand, within GFF2821 at 342.812 kb on - strand, within GFF2821 at 342.939 kb on + strand at 342.940 kb on - strand at 343.026 kb on - strand at 343.744 kb on - strand at 343.769 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 341,486 + GFF2820 0.59 +0.7 341,537 + GFF2820 0.62 -1.1 341,539 + GFF2820 0.62 -0.6 341,694 - GFF2820 0.71 -0.1 341,736 - GFF2820 0.74 +0.5 341,764 - GFF2820 0.76 -2.2 341,789 + GFF2820 0.77 -3.3 341,905 - GFF2820 0.84 +1.4 342,033 + -2.3 342,424 + -0.5 342,424 + -0.2 342,561 - GFF2821 0.31 +0.9 342,563 - GFF2821 0.31 +0.7 342,621 + GFF2821 0.41 -1.7 342,622 - GFF2821 0.41 -4.5 342,622 - GFF2821 0.41 +0.6 342,812 - GFF2821 0.74 +1.4 342,939 + -3.2 342,940 - +0.7 343,026 - -0.8 343,744 - +1.5 343,769 - -0.8
Or see this region's nucleotide sequence