Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2767 and GFF2768 are separated by 79 nucleotides GFF2768 and GFF2769 are separated by 266 nucleotides GFF2769 and GFF2770 are separated by 53 nucleotides GFF2770 and GFF2771 are separated by 37 nucleotides
GFF2767 - Putative ACR protein, at 293,592 to 293,945
GFF2767
GFF2768 - Cation transport regulator chaB, at 294,025 to 294,255
GFF2768
GFF2769 - Calcium/proton antiporter, at 294,522 to 295,622
GFF2769
GFF2770 - 2-Keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase (EC 2.5.1.55), at 295,676 to 296,530
GFF2770
GFF2771 - FIG002708: Protein SirB1, at 296,568 to 297,377
GFF2771
Position (kb) 294
295
296 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 293.588 kb on - strand at 293.649 kb on - strand, within GFF2767 at 293.649 kb on - strand, within GFF2767 at 293.771 kb on + strand, within GFF2767 at 294.168 kb on + strand, within GFF2768 at 294.736 kb on - strand, within GFF2769 at 294.751 kb on + strand, within GFF2769 at 294.827 kb on + strand, within GFF2769 at 294.828 kb on - strand, within GFF2769 at 295.112 kb on + strand, within GFF2769 at 295.117 kb on - strand, within GFF2769 at 295.463 kb on + strand, within GFF2769 at 295.463 kb on + strand, within GFF2769 at 295.501 kb on + strand, within GFF2769
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 293,588 - -1.2 293,649 - GFF2767 0.16 -1.7 293,649 - GFF2767 0.16 +0.3 293,771 + GFF2767 0.51 +0.4 294,168 + GFF2768 0.62 +0.2 294,736 - GFF2769 0.19 +0.6 294,751 + GFF2769 0.21 -0.6 294,827 + GFF2769 0.28 -0.4 294,828 - GFF2769 0.28 -2.6 295,112 + GFF2769 0.54 -3.0 295,117 - GFF2769 0.54 -1.1 295,463 + GFF2769 0.85 +0.8 295,463 + GFF2769 0.85 -0.7 295,501 + GFF2769 0.89 -0.2
Or see this region's nucleotide sequence