Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2763 and GFF2764 are separated by 340 nucleotides GFF2764 and GFF2765 overlap by 8 nucleotides GFF2765 and GFF2766 are separated by 0 nucleotides
GFF2763 - Nitrate/nitrite transporter, at 287,813 to 289,210
GFF2763
GFF2764 - Nitrate/nitrite sensor protein (EC 2.7.3.-), at 289,551 to 291,347
GFF2764
GFF2765 - Nitrate/nitrite response regulator protein, at 291,340 to 291,990
GFF2765
GFF2766 - Invasin, at 291,991 to 293,415
GFF2766
Position (kb) 289
290
291
292 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 288.606 kb on - strand, within GFF2763 at 288.634 kb on + strand, within GFF2763 at 288.937 kb on - strand, within GFF2763 at 288.985 kb on + strand, within GFF2763 at 289.102 kb on + strand at 289.453 kb on + strand at 289.548 kb on - strand at 289.557 kb on - strand at 290.009 kb on - strand, within GFF2764 at 290.009 kb on - strand, within GFF2764 at 290.012 kb on - strand, within GFF2764 at 290.044 kb on + strand, within GFF2764 at 290.057 kb on + strand, within GFF2764 at 290.142 kb on - strand, within GFF2764 at 290.201 kb on + strand, within GFF2764 at 290.202 kb on - strand, within GFF2764 at 290.204 kb on + strand, within GFF2764 at 290.205 kb on - strand, within GFF2764 at 290.230 kb on - strand, within GFF2764 at 290.280 kb on - strand, within GFF2764 at 290.864 kb on - strand, within GFF2764 at 290.864 kb on - strand, within GFF2764 at 291.300 kb on - strand at 291.335 kb on + strand at 291.585 kb on + strand, within GFF2765 at 291.656 kb on - strand, within GFF2765 at 291.728 kb on + strand, within GFF2765 at 291.728 kb on + strand, within GFF2765 at 291.825 kb on + strand, within GFF2765 at 291.919 kb on - strand, within GFF2765 at 291.992 kb on + strand at 291.992 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 288,606 - GFF2763 0.57 -2.3 288,634 + GFF2763 0.59 +0.2 288,937 - GFF2763 0.80 -0.2 288,985 + GFF2763 0.84 -0.0 289,102 + +0.7 289,453 + -0.5 289,548 - -0.5 289,557 - -1.0 290,009 - GFF2764 0.25 -2.0 290,009 - GFF2764 0.25 +0.5 290,012 - GFF2764 0.26 -0.7 290,044 + GFF2764 0.27 -1.7 290,057 + GFF2764 0.28 +1.0 290,142 - GFF2764 0.33 +0.3 290,201 + GFF2764 0.36 -1.3 290,202 - GFF2764 0.36 -0.8 290,204 + GFF2764 0.36 -0.9 290,205 - GFF2764 0.36 -0.4 290,230 - GFF2764 0.38 +0.6 290,280 - GFF2764 0.41 +0.3 290,864 - GFF2764 0.73 +0.2 290,864 - GFF2764 0.73 -2.9 291,300 - +0.9 291,335 + -0.8 291,585 + GFF2765 0.38 -0.9 291,656 - GFF2765 0.49 +0.3 291,728 + GFF2765 0.60 +0.9 291,728 + GFF2765 0.60 -0.4 291,825 + GFF2765 0.75 +0.5 291,919 - GFF2765 0.89 -0.5 291,992 + +0.5 291,992 + -0.2
Or see this region's nucleotide sequence