Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2402 and GFF2403 are separated by 165 nucleotides GFF2403 and GFF2404 are separated by 137 nucleotides
GFF2402 - RatA homolog, at 2,886 to 10,193
GFF2402
GFF2403 - RatA homolog, at 10,359 to 15,938
GFF2403
GFF2404 - SinI homolog, at 16,076 to 17,035
GFF2404
Position (kb)
10
11
12
13
14
15
16 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 9.660 kb on - strand at 9.662 kb on - strand at 9.662 kb on - strand at 9.662 kb on - strand at 9.772 kb on - strand at 9.772 kb on - strand at 9.772 kb on - strand at 9.772 kb on - strand at 9.773 kb on + strand at 9.773 kb on + strand at 9.887 kb on + strand at 9.915 kb on + strand at 10.062 kb on - strand at 10.199 kb on + strand at 10.200 kb on - strand at 10.283 kb on - strand at 10.448 kb on - strand at 10.451 kb on - strand at 10.451 kb on - strand at 10.454 kb on + strand at 10.454 kb on + strand at 10.563 kb on - strand at 10.621 kb on + strand at 10.622 kb on - strand at 10.625 kb on - strand at 10.753 kb on + strand at 10.754 kb on - strand at 10.754 kb on - strand at 10.754 kb on - strand at 10.791 kb on - strand at 10.804 kb on - strand at 10.804 kb on - strand at 10.804 kb on - strand at 10.898 kb on + strand at 10.950 kb on - strand, within GFF2403 at 10.953 kb on - strand, within GFF2403 at 11.053 kb on + strand, within GFF2403 at 11.053 kb on + strand, within GFF2403 at 11.054 kb on - strand, within GFF2403 at 11.087 kb on - strand, within GFF2403 at 11.270 kb on - strand, within GFF2403 at 11.324 kb on + strand, within GFF2403 at 11.324 kb on + strand, within GFF2403 at 11.327 kb on - strand, within GFF2403 at 11.455 kb on - strand, within GFF2403 at 11.455 kb on - strand, within GFF2403 at 11.489 kb on - strand, within GFF2403 at 11.492 kb on + strand, within GFF2403 at 11.502 kb on - strand, within GFF2403 at 11.502 kb on - strand, within GFF2403 at 11.542 kb on + strand, within GFF2403 at 11.542 kb on + strand, within GFF2403 at 11.542 kb on + strand, within GFF2403 at 11.542 kb on + strand, within GFF2403 at 11.641 kb on + strand, within GFF2403 at 11.642 kb on - strand, within GFF2403 at 11.642 kb on - strand, within GFF2403 at 11.754 kb on + strand, within GFF2403 at 11.789 kb on + strand, within GFF2403 at 11.833 kb on + strand, within GFF2403 at 11.905 kb on + strand, within GFF2403 at 12.039 kb on - strand, within GFF2403 at 12.071 kb on + strand, within GFF2403 at 12.182 kb on - strand, within GFF2403 at 12.184 kb on + strand, within GFF2403 at 12.274 kb on - strand, within GFF2403 at 12.448 kb on + strand, within GFF2403 at 12.504 kb on - strand, within GFF2403 at 12.555 kb on + strand, within GFF2403 at 12.592 kb on + strand, within GFF2403 at 12.771 kb on - strand, within GFF2403 at 12.773 kb on - strand, within GFF2403 at 13.073 kb on - strand, within GFF2403 at 13.091 kb on + strand, within GFF2403 at 13.091 kb on + strand, within GFF2403 at 13.091 kb on + strand, within GFF2403 at 13.092 kb on - strand, within GFF2403 at 13.092 kb on - strand, within GFF2403 at 13.145 kb on + strand, within GFF2403 at 13.148 kb on - strand, within GFF2403 at 13.332 kb on - strand, within GFF2403 at 13.448 kb on + strand, within GFF2403 at 13.586 kb on + strand, within GFF2403 at 13.951 kb on + strand, within GFF2403 at 14.135 kb on + strand, within GFF2403 at 14.322 kb on - strand, within GFF2403 at 14.323 kb on + strand, within GFF2403 at 14.478 kb on + strand, within GFF2403 at 14.478 kb on + strand, within GFF2403 at 14.521 kb on + strand, within GFF2403 at 14.521 kb on + strand, within GFF2403 at 14.521 kb on + strand, within GFF2403 at 14.522 kb on - strand, within GFF2403 at 14.544 kb on - strand, within GFF2403 at 14.636 kb on + strand, within GFF2403 at 14.637 kb on - strand, within GFF2403 at 14.640 kb on + strand, within GFF2403 at 14.640 kb on + strand, within GFF2403 at 14.641 kb on - strand, within GFF2403 at 14.896 kb on + strand, within GFF2403 at 14.903 kb on + strand, within GFF2403 at 14.948 kb on + strand, within GFF2403 at 15.390 kb on + strand at 15.432 kb on - strand at 15.432 kb on - strand at 15.761 kb on + strand at 16.077 kb on + strand at 16.089 kb on + strand at 16.243 kb on + strand, within GFF2404 at 16.282 kb on - strand, within GFF2404 at 16.317 kb on + strand, within GFF2404 at 16.317 kb on + strand, within GFF2404 at 16.317 kb on + strand, within GFF2404 at 16.317 kb on + strand, within GFF2404 at 16.321 kb on + strand, within GFF2404 at 16.353 kb on - strand, within GFF2404 at 16.353 kb on - strand, within GFF2404 at 16.359 kb on + strand, within GFF2404 at 16.360 kb on - strand, within GFF2404 at 16.555 kb on + strand, within GFF2404 at 16.585 kb on - strand, within GFF2404 at 16.757 kb on - strand, within GFF2404 at 16.816 kb on + strand, within GFF2404 at 16.817 kb on - strand, within GFF2404 at 16.826 kb on + strand, within GFF2404 at 16.826 kb on + strand, within GFF2404
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 9,660 - -0.5 9,662 - +0.0 9,662 - -3.7 9,662 - +0.1 9,772 - -2.7 9,772 - -2.1 9,772 - -0.2 9,772 - +0.1 9,773 + +0.3 9,773 + -3.3 9,887 + +0.8 9,915 + -0.5 10,062 - -0.2 10,199 + -0.1 10,200 - -0.4 10,283 - +0.3 10,448 - -0.9 10,451 - +1.2 10,451 - +0.7 10,454 + -1.1 10,454 + -2.4 10,563 - +0.4 10,621 + +0.3 10,622 - -0.1 10,625 - -1.2 10,753 + -0.4 10,754 - +0.3 10,754 - +1.3 10,754 - +0.7 10,791 - +1.4 10,804 - +0.4 10,804 - +0.8 10,804 - +0.2 10,898 + -0.2 10,950 - GFF2403 0.11 +0.7 10,953 - GFF2403 0.11 -3.8 11,053 + GFF2403 0.12 +1.4 11,053 + GFF2403 0.12 +0.3 11,054 - GFF2403 0.12 -3.9 11,087 - GFF2403 0.13 +0.8 11,270 - GFF2403 0.16 +1.5 11,324 + GFF2403 0.17 +0.6 11,324 + GFF2403 0.17 -0.2 11,327 - GFF2403 0.17 +0.2 11,455 - GFF2403 0.20 +1.3 11,455 - GFF2403 0.20 +1.3 11,489 - GFF2403 0.20 +0.7 11,492 + GFF2403 0.20 +1.0 11,502 - GFF2403 0.20 +1.2 11,502 - GFF2403 0.20 -0.0 11,542 + GFF2403 0.21 -3.3 11,542 + GFF2403 0.21 -1.7 11,542 + GFF2403 0.21 +1.2 11,542 + GFF2403 0.21 +0.2 11,641 + GFF2403 0.23 +0.8 11,642 - GFF2403 0.23 +1.4 11,642 - GFF2403 0.23 +1.0 11,754 + GFF2403 0.25 +4.4 11,789 + GFF2403 0.26 +0.9 11,833 + GFF2403 0.26 -1.4 11,905 + GFF2403 0.28 +0.7 12,039 - GFF2403 0.30 +0.8 12,071 + GFF2403 0.31 -0.9 12,182 - GFF2403 0.33 -1.8 12,184 + GFF2403 0.33 +1.0 12,274 - GFF2403 0.34 +0.6 12,448 + GFF2403 0.37 -0.5 12,504 - GFF2403 0.38 -0.2 12,555 + GFF2403 0.39 +0.5 12,592 + GFF2403 0.40 +0.5 12,771 - GFF2403 0.43 +1.0 12,773 - GFF2403 0.43 -2.2 13,073 - GFF2403 0.49 +0.4 13,091 + GFF2403 0.49 +0.6 13,091 + GFF2403 0.49 +1.3 13,091 + GFF2403 0.49 +1.5 13,092 - GFF2403 0.49 -2.4 13,092 - GFF2403 0.49 +1.8 13,145 + GFF2403 0.50 +0.2 13,148 - GFF2403 0.50 -2.4 13,332 - GFF2403 0.53 -1.2 13,448 + GFF2403 0.55 +1.4 13,586 + GFF2403 0.58 +0.3 13,951 + GFF2403 0.64 +1.2 14,135 + GFF2403 0.68 +1.5 14,322 - GFF2403 0.71 +2.4 14,323 + GFF2403 0.71 -0.4 14,478 + GFF2403 0.74 +2.1 14,478 + GFF2403 0.74 +0.2 14,521 + GFF2403 0.75 +0.9 14,521 + GFF2403 0.75 +1.9 14,521 + GFF2403 0.75 +0.9 14,522 - GFF2403 0.75 +0.5 14,544 - GFF2403 0.75 +1.3 14,636 + GFF2403 0.77 +0.6 14,637 - GFF2403 0.77 -0.5 14,640 + GFF2403 0.77 -1.5 14,640 + GFF2403 0.77 -1.9 14,641 - GFF2403 0.77 +0.4 14,896 + GFF2403 0.81 -3.1 14,903 + GFF2403 0.81 +1.1 14,948 + GFF2403 0.82 +1.1 15,390 + +1.5 15,432 - +0.4 15,432 - +1.2 15,761 + -0.1 16,077 + +0.7 16,089 + -0.1 16,243 + GFF2404 0.17 +0.6 16,282 - GFF2404 0.21 -0.9 16,317 + GFF2404 0.25 -2.8 16,317 + GFF2404 0.25 +0.0 16,317 + GFF2404 0.25 +0.1 16,317 + GFF2404 0.25 -2.9 16,321 + GFF2404 0.26 +0.5 16,353 - GFF2404 0.29 -1.4 16,353 - GFF2404 0.29 -2.2 16,359 + GFF2404 0.29 +0.5 16,360 - GFF2404 0.30 -3.8 16,555 + GFF2404 0.50 +1.7 16,585 - GFF2404 0.53 +1.3 16,757 - GFF2404 0.71 +0.5 16,816 + GFF2404 0.77 +1.1 16,817 - GFF2404 0.77 +0.8 16,826 + GFF2404 0.78 -2.7 16,826 + GFF2404 0.78 -1.8
Or see this region's nucleotide sequence