Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2402 and GFF2403 are separated by 165 nucleotides
GFF2402 - RatA homolog, at 2,886 to 10,193
GFF2402
GFF2403 - RatA homolog, at 10,359 to 15,938
GFF2403
Position (kb)
2
3
4
5
6
7
8
9
10
11 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1.903 kb on + strand at 1.906 kb on - strand at 1.906 kb on - strand at 1.934 kb on - strand at 1.984 kb on + strand at 1.989 kb on + strand at 2.127 kb on + strand at 2.180 kb on - strand at 2.183 kb on + strand at 2.705 kb on + strand at 2.705 kb on + strand at 2.707 kb on + strand at 2.709 kb on + strand at 2.709 kb on + strand at 2.880 kb on - strand at 2.882 kb on + strand at 2.882 kb on + strand at 2.882 kb on + strand at 2.882 kb on + strand at 2.882 kb on + strand at 2.927 kb on + strand at 2.971 kb on + strand at 3.049 kb on + strand at 3.092 kb on + strand at 3.180 kb on - strand at 3.184 kb on - strand at 3.184 kb on - strand at 3.266 kb on + strand at 3.267 kb on - strand at 3.306 kb on - strand at 3.348 kb on - strand at 3.411 kb on - strand at 3.470 kb on + strand at 3.503 kb on + strand at 3.561 kb on - strand at 3.629 kb on + strand, within GFF2402 at 3.722 kb on + strand, within GFF2402 at 3.735 kb on - strand, within GFF2402 at 3.740 kb on + strand, within GFF2402 at 3.820 kb on - strand, within GFF2402 at 3.822 kb on - strand, within GFF2402 at 3.830 kb on - strand, within GFF2402 at 3.896 kb on - strand, within GFF2402 at 3.931 kb on + strand, within GFF2402 at 3.931 kb on + strand, within GFF2402 at 3.932 kb on - strand, within GFF2402 at 4.045 kb on + strand, within GFF2402 at 4.045 kb on + strand, within GFF2402 at 4.050 kb on - strand, within GFF2402 at 4.141 kb on + strand, within GFF2402 at 4.142 kb on - strand, within GFF2402 at 4.199 kb on + strand, within GFF2402 at 4.302 kb on - strand, within GFF2402 at 4.383 kb on + strand, within GFF2402 at 4.576 kb on - strand, within GFF2402 at 4.806 kb on + strand, within GFF2402 at 4.806 kb on + strand, within GFF2402 at 4.878 kb on - strand, within GFF2402 at 4.878 kb on - strand, within GFF2402 at 4.971 kb on - strand, within GFF2402 at 4.994 kb on + strand, within GFF2402 at 4.994 kb on + strand, within GFF2402 at 5.005 kb on + strand, within GFF2402 at 5.174 kb on - strand, within GFF2402 at 5.174 kb on - strand, within GFF2402 at 5.174 kb on - strand, within GFF2402 at 5.204 kb on - strand, within GFF2402 at 5.482 kb on + strand, within GFF2402 at 5.482 kb on + strand, within GFF2402 at 5.485 kb on + strand, within GFF2402 at 5.582 kb on - strand, within GFF2402 at 5.863 kb on + strand, within GFF2402 at 5.911 kb on + strand, within GFF2402 at 6.015 kb on - strand, within GFF2402 at 6.083 kb on - strand, within GFF2402 at 6.083 kb on - strand, within GFF2402 at 6.083 kb on - strand, within GFF2402 at 6.123 kb on + strand, within GFF2402 at 6.123 kb on + strand, within GFF2402 at 6.143 kb on + strand, within GFF2402 at 6.295 kb on + strand, within GFF2402 at 6.296 kb on - strand, within GFF2402 at 6.296 kb on - strand, within GFF2402 at 6.316 kb on - strand, within GFF2402 at 6.407 kb on + strand, within GFF2402 at 6.562 kb on + strand, within GFF2402 at 6.776 kb on + strand, within GFF2402 at 6.825 kb on - strand, within GFF2402 at 7.130 kb on + strand, within GFF2402 at 7.131 kb on - strand, within GFF2402 at 7.132 kb on + strand, within GFF2402 at 7.233 kb on - strand, within GFF2402 at 7.357 kb on + strand, within GFF2402 at 7.357 kb on + strand, within GFF2402 at 7.358 kb on - strand, within GFF2402 at 7.556 kb on + strand, within GFF2402 at 7.557 kb on - strand, within GFF2402 at 7.657 kb on - strand, within GFF2402 at 8.023 kb on + strand, within GFF2402 at 8.024 kb on - strand, within GFF2402 at 8.112 kb on - strand, within GFF2402 at 8.113 kb on + strand, within GFF2402 at 8.113 kb on + strand, within GFF2402 at 8.250 kb on - strand, within GFF2402 at 8.361 kb on - strand, within GFF2402 at 8.470 kb on - strand, within GFF2402 at 8.571 kb on + strand, within GFF2402 at 8.668 kb on + strand, within GFF2402 at 8.826 kb on - strand, within GFF2402 at 8.876 kb on + strand, within GFF2402 at 8.876 kb on + strand, within GFF2402 at 8.876 kb on + strand, within GFF2402 at 8.877 kb on - strand, within GFF2402 at 9.029 kb on + strand, within GFF2402 at 9.145 kb on + strand, within GFF2402 at 9.145 kb on + strand, within GFF2402 at 9.194 kb on - strand, within GFF2402 at 9.660 kb on - strand at 9.662 kb on - strand at 9.662 kb on - strand at 9.662 kb on - strand at 9.772 kb on - strand at 9.772 kb on - strand at 9.772 kb on - strand at 9.772 kb on - strand at 9.773 kb on + strand at 9.773 kb on + strand at 9.887 kb on + strand at 9.915 kb on + strand at 10.062 kb on - strand at 10.199 kb on + strand at 10.200 kb on - strand at 10.283 kb on - strand at 10.448 kb on - strand at 10.451 kb on - strand at 10.451 kb on - strand at 10.454 kb on + strand at 10.454 kb on + strand at 10.563 kb on - strand at 10.621 kb on + strand at 10.622 kb on - strand at 10.625 kb on - strand at 10.753 kb on + strand at 10.754 kb on - strand at 10.754 kb on - strand at 10.754 kb on - strand at 10.791 kb on - strand at 10.804 kb on - strand at 10.804 kb on - strand at 10.804 kb on - strand at 10.898 kb on + strand at 10.950 kb on - strand, within GFF2403 at 10.953 kb on - strand, within GFF2403 at 11.053 kb on + strand, within GFF2403 at 11.053 kb on + strand, within GFF2403 at 11.054 kb on - strand, within GFF2403 at 11.087 kb on - strand, within GFF2403
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 1,903 + -1.4 1,906 - +0.8 1,906 - -0.4 1,934 - -0.8 1,984 + -3.9 1,989 + -0.3 2,127 + -0.9 2,180 - -2.3 2,183 + +0.9 2,705 + +1.4 2,705 + -1.3 2,707 + -2.5 2,709 + +0.8 2,709 + +0.6 2,880 - +0.2 2,882 + +1.2 2,882 + -0.9 2,882 + -1.1 2,882 + -0.3 2,882 + +1.6 2,927 + +0.6 2,971 + -4.0 3,049 + -0.3 3,092 + -0.5 3,180 - +1.5 3,184 - -0.1 3,184 - +0.3 3,266 + +0.2 3,267 - +0.5 3,306 - +0.6 3,348 - -1.3 3,411 - +0.7 3,470 + +0.2 3,503 + +0.6 3,561 - -0.7 3,629 + GFF2402 0.10 -0.0 3,722 + GFF2402 0.11 +0.7 3,735 - GFF2402 0.12 +0.4 3,740 + GFF2402 0.12 +1.3 3,820 - GFF2402 0.13 +4.0 3,822 - GFF2402 0.13 -0.1 3,830 - GFF2402 0.13 -1.9 3,896 - GFF2402 0.14 -0.8 3,931 + GFF2402 0.14 -0.2 3,931 + GFF2402 0.14 +0.8 3,932 - GFF2402 0.14 +0.3 4,045 + GFF2402 0.16 +0.8 4,045 + GFF2402 0.16 +4.1 4,050 - GFF2402 0.16 -4.0 4,141 + GFF2402 0.17 +1.7 4,142 - GFF2402 0.17 +0.6 4,199 + GFF2402 0.18 +1.0 4,302 - GFF2402 0.19 -0.9 4,383 + GFF2402 0.20 +0.2 4,576 - GFF2402 0.23 +0.7 4,806 + GFF2402 0.26 +0.6 4,806 + GFF2402 0.26 -0.0 4,878 - GFF2402 0.27 +0.7 4,878 - GFF2402 0.27 +0.5 4,971 - GFF2402 0.29 +0.1 4,994 + GFF2402 0.29 +0.4 4,994 + GFF2402 0.29 -2.6 5,005 + GFF2402 0.29 +1.7 5,174 - GFF2402 0.31 -2.4 5,174 - GFF2402 0.31 -0.5 5,174 - GFF2402 0.31 -1.4 5,204 - GFF2402 0.32 -2.8 5,482 + GFF2402 0.36 +0.7 5,482 + GFF2402 0.36 -0.3 5,485 + GFF2402 0.36 -1.3 5,582 - GFF2402 0.37 -0.4 5,863 + GFF2402 0.41 -2.8 5,911 + GFF2402 0.41 +0.1 6,015 - GFF2402 0.43 +0.5 6,083 - GFF2402 0.44 +0.1 6,083 - GFF2402 0.44 +0.5 6,083 - GFF2402 0.44 +0.6 6,123 + GFF2402 0.44 -0.6 6,123 + GFF2402 0.44 +1.6 6,143 + GFF2402 0.45 +2.0 6,295 + GFF2402 0.47 +0.2 6,296 - GFF2402 0.47 -1.6 6,296 - GFF2402 0.47 -1.1 6,316 - GFF2402 0.47 +0.9 6,407 + GFF2402 0.48 -1.3 6,562 + GFF2402 0.50 +0.3 6,776 + GFF2402 0.53 +0.7 6,825 - GFF2402 0.54 +1.3 7,130 + GFF2402 0.58 -0.3 7,131 - GFF2402 0.58 +0.6 7,132 + GFF2402 0.58 +0.2 7,233 - GFF2402 0.59 +0.2 7,357 + GFF2402 0.61 +0.7 7,357 + GFF2402 0.61 +0.9 7,358 - GFF2402 0.61 +0.3 7,556 + GFF2402 0.64 +0.9 7,557 - GFF2402 0.64 -0.5 7,657 - GFF2402 0.65 +0.4 8,023 + GFF2402 0.70 +0.8 8,024 - GFF2402 0.70 +0.4 8,112 - GFF2402 0.72 +1.6 8,113 + GFF2402 0.72 +1.9 8,113 + GFF2402 0.72 +0.4 8,250 - GFF2402 0.73 -2.5 8,361 - GFF2402 0.75 +0.0 8,470 - GFF2402 0.76 +0.4 8,571 + GFF2402 0.78 -0.4 8,668 + GFF2402 0.79 -1.5 8,826 - GFF2402 0.81 +0.5 8,876 + GFF2402 0.82 +0.4 8,876 + GFF2402 0.82 +0.3 8,876 + GFF2402 0.82 +1.6 8,877 - GFF2402 0.82 -1.9 9,029 + GFF2402 0.84 -3.7 9,145 + GFF2402 0.86 -0.4 9,145 + GFF2402 0.86 -0.4 9,194 - GFF2402 0.86 +0.9 9,660 - -0.5 9,662 - +0.0 9,662 - -3.7 9,662 - +0.1 9,772 - -2.7 9,772 - -2.1 9,772 - -0.2 9,772 - +0.1 9,773 + +0.3 9,773 + -3.3 9,887 + +0.8 9,915 + -0.5 10,062 - -0.2 10,199 + -0.1 10,200 - -0.4 10,283 - +0.3 10,448 - -0.9 10,451 - +1.2 10,451 - +0.7 10,454 + -1.1 10,454 + -2.4 10,563 - +0.4 10,621 + +0.3 10,622 - -0.1 10,625 - -1.2 10,753 + -0.4 10,754 - +0.3 10,754 - +1.3 10,754 - +0.7 10,791 - +1.4 10,804 - +0.4 10,804 - +0.8 10,804 - +0.2 10,898 + -0.2 10,950 - GFF2403 0.11 +0.7 10,953 - GFF2403 0.11 -3.8 11,053 + GFF2403 0.12 +1.4 11,053 + GFF2403 0.12 +0.3 11,054 - GFF2403 0.12 -3.9 11,087 - GFF2403 0.13 +0.8
Or see this region's nucleotide sequence