Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2357

Experiment: 6H2_phage 10 MOI; agar plate assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2356 and GFF2357 are separated by 72 nucleotidesGFF2357 and GFF2358 overlap by 4 nucleotidesGFF2358 and GFF2359 are separated by 379 nucleotides GFF2356 - Bacterioferritin-associated ferredoxin, at 247 to 441 GFF2356 GFF2357 - Bacterioferritin, at 514 to 990 GFF2357 GFF2358 - Leader peptidase (Prepilin peptidase) (EC 3.4.23.43) / N-methyltransferase (EC 2.1.1.-), at 987 to 1,454 GFF2358 GFF2359 - SSU ribosomal protein S10p (S20e), at 1,834 to 2,145 GFF2359 Position (kb) 0 1Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 0.250 kb on + strandat 0.264 kb on + strandat 0.271 kb on - strand, within GFF2356at 0.511 kb on - strandat 0.550 kb on - strandat 0.586 kb on + strand, within GFF2357at 0.681 kb on + strand, within GFF2357at 0.685 kb on + strand, within GFF2357at 0.685 kb on + strand, within GFF2357at 0.685 kb on + strand, within GFF2357at 0.686 kb on - strand, within GFF2357at 0.686 kb on - strand, within GFF2357at 0.686 kb on - strand, within GFF2357at 0.687 kb on + strand, within GFF2357at 0.687 kb on + strand, within GFF2357at 0.717 kb on - strand, within GFF2357at 0.833 kb on - strand, within GFF2357at 0.853 kb on + strand, within GFF2357at 0.867 kb on + strand, within GFF2357at 0.976 kb on - strandat 1.021 kb on - strandat 1.069 kb on - strand, within GFF2358at 1.190 kb on - strand, within GFF2358at 1.394 kb on - strand, within GFF2358at 1.394 kb on - strand, within GFF2358at 1.405 kb on - strand, within GFF2358at 1.405 kb on - strand, within GFF2358at 1.490 kb on + strandat 1.497 kb on - strandat 1.497 kb on - strandat 1.518 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay
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250 + +0.0
264 + -0.4
271 - GFF2356 0.12 -0.6
511 - -0.3
550 - -1.6
586 + GFF2357 0.15 +1.6
681 + GFF2357 0.35 +0.2
685 + GFF2357 0.36 -0.5
685 + GFF2357 0.36 -4.1
685 + GFF2357 0.36 -0.5
686 - GFF2357 0.36 -0.7
686 - GFF2357 0.36 -0.0
686 - GFF2357 0.36 +0.6
687 + GFF2357 0.36 +0.1
687 + GFF2357 0.36 +0.9
717 - GFF2357 0.43 +0.4
833 - GFF2357 0.67 +0.8
853 + GFF2357 0.71 +1.4
867 + GFF2357 0.74 +0.7
976 - -0.3
1,021 - -0.5
1,069 - GFF2358 0.18 -0.1
1,190 - GFF2358 0.43 -1.9
1,394 - GFF2358 0.87 +1.0
1,394 - GFF2358 0.87 +0.6
1,405 - GFF2358 0.89 +0.8
1,405 - GFF2358 0.89 +1.2
1,490 + +0.9
1,497 - +0.9
1,497 - +0.2
1,518 - -0.1

Or see this region's nucleotide sequence