Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2324

Experiment: 6H2_phage 10 MOI; agar plate assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2323 and GFF2324 are separated by 2 nucleotidesGFF2324 and GFF2325 are separated by 115 nucleotidesGFF2325 and GFF2326 are separated by 2 nucleotides GFF2323 - Cystathionine gamma-synthase (EC 2.5.1.48), at 108,637 to 109,797 GFF2323 GFF2324 - Aspartokinase (EC 2.7.2.4) / Homoserine dehydrogenase (EC 1.1.1.3), at 109,800 to 112,232 GFF2324 GFF2325 - Small-conductance mechanosensitive channel, at 112,348 to 113,205 GFF2325 GFF2326 - FIG01046298: hypothetical protein, at 113,208 to 114,206 GFF2326 Position (kb) 109 110 111 112 113Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 108.848 kb on + strand, within GFF2323at 108.949 kb on + strand, within GFF2323at 109.155 kb on + strand, within GFF2323at 109.275 kb on + strand, within GFF2323at 109.347 kb on + strand, within GFF2323at 109.348 kb on - strand, within GFF2323at 109.351 kb on + strand, within GFF2323at 109.352 kb on - strand, within GFF2323at 109.352 kb on - strand, within GFF2323at 109.390 kb on - strand, within GFF2323at 109.720 kb on - strandat 109.752 kb on - strandat 109.859 kb on + strandat 109.955 kb on + strandat 109.973 kb on + strandat 110.023 kb on + strandat 110.092 kb on + strand, within GFF2324at 110.093 kb on - strand, within GFF2324at 110.105 kb on + strand, within GFF2324at 110.164 kb on - strand, within GFF2324at 110.311 kb on + strand, within GFF2324at 110.336 kb on - strand, within GFF2324at 110.414 kb on - strand, within GFF2324at 110.506 kb on - strand, within GFF2324at 110.661 kb on - strand, within GFF2324at 110.661 kb on - strand, within GFF2324at 110.664 kb on + strand, within GFF2324at 110.747 kb on + strand, within GFF2324at 110.747 kb on + strand, within GFF2324at 110.748 kb on - strand, within GFF2324at 110.748 kb on - strand, within GFF2324at 110.875 kb on + strand, within GFF2324at 110.909 kb on + strand, within GFF2324at 111.297 kb on - strand, within GFF2324at 111.542 kb on + strand, within GFF2324at 111.542 kb on + strand, within GFF2324at 111.546 kb on + strand, within GFF2324at 111.546 kb on + strand, within GFF2324at 111.594 kb on + strand, within GFF2324at 111.595 kb on - strand, within GFF2324at 111.596 kb on + strand, within GFF2324at 111.728 kb on + strand, within GFF2324at 111.883 kb on + strand, within GFF2324at 111.884 kb on - strand, within GFF2324at 111.920 kb on + strand, within GFF2324at 111.920 kb on + strand, within GFF2324at 112.103 kb on + strandat 112.234 kb on - strandat 112.310 kb on - strandat 112.310 kb on - strandat 112.315 kb on + strandat 112.316 kb on - strandat 112.317 kb on + strandat 112.318 kb on - strandat 112.405 kb on - strandat 112.457 kb on + strand, within GFF2325at 112.457 kb on + strand, within GFF2325at 112.457 kb on + strand, within GFF2325at 112.458 kb on - strand, within GFF2325at 112.557 kb on + strand, within GFF2325at 112.582 kb on - strand, within GFF2325at 112.591 kb on + strand, within GFF2325at 112.591 kb on + strand, within GFF2325at 112.798 kb on + strand, within GFF2325at 112.933 kb on + strand, within GFF2325at 112.934 kb on - strand, within GFF2325at 113.075 kb on - strand, within GFF2325

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Per-strain Table

Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay
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108,848 + GFF2323 0.18 -1.2
108,949 + GFF2323 0.27 -1.5
109,155 + GFF2323 0.45 -3.1
109,275 + GFF2323 0.55 -4.2
109,347 + GFF2323 0.61 +0.8
109,348 - GFF2323 0.61 -1.9
109,351 + GFF2323 0.61 -3.2
109,352 - GFF2323 0.62 -3.2
109,352 - GFF2323 0.62 -0.3
109,390 - GFF2323 0.65 +0.4
109,720 - +1.4
109,752 - -0.2
109,859 + +0.3
109,955 + -2.3
109,973 + +3.3
110,023 + +2.0
110,092 + GFF2324 0.12 +0.5
110,093 - GFF2324 0.12 -0.0
110,105 + GFF2324 0.13 -4.6
110,164 - GFF2324 0.15 -0.5
110,311 + GFF2324 0.21 -3.3
110,336 - GFF2324 0.22 -2.2
110,414 - GFF2324 0.25 +1.1
110,506 - GFF2324 0.29 -0.4
110,661 - GFF2324 0.35 +1.0
110,661 - GFF2324 0.35 +0.3
110,664 + GFF2324 0.36 -2.0
110,747 + GFF2324 0.39 +0.0
110,747 + GFF2324 0.39 -0.1
110,748 - GFF2324 0.39 +0.2
110,748 - GFF2324 0.39 -1.2
110,875 + GFF2324 0.44 -0.3
110,909 + GFF2324 0.46 -0.4
111,297 - GFF2324 0.62 +1.1
111,542 + GFF2324 0.72 +0.3
111,542 + GFF2324 0.72 -0.6
111,546 + GFF2324 0.72 -0.7
111,546 + GFF2324 0.72 -0.3
111,594 + GFF2324 0.74 -0.5
111,595 - GFF2324 0.74 -0.1
111,596 + GFF2324 0.74 +0.5
111,728 + GFF2324 0.79 +0.4
111,883 + GFF2324 0.86 -0.8
111,884 - GFF2324 0.86 -2.1
111,920 + GFF2324 0.87 -1.1
111,920 + GFF2324 0.87 +0.0
112,103 + +1.7
112,234 - +0.4
112,310 - -0.6
112,310 - -3.1
112,315 + -0.3
112,316 - -0.2
112,317 + +0.1
112,318 - +0.3
112,405 - +0.4
112,457 + GFF2325 0.13 +0.1
112,457 + GFF2325 0.13 +1.3
112,457 + GFF2325 0.13 -2.5
112,458 - GFF2325 0.13 -0.0
112,557 + GFF2325 0.24 -1.4
112,582 - GFF2325 0.27 +0.9
112,591 + GFF2325 0.28 -4.1
112,591 + GFF2325 0.28 +0.2
112,798 + GFF2325 0.52 -0.6
112,933 + GFF2325 0.68 -0.3
112,934 - GFF2325 0.68 -2.1
113,075 - GFF2325 0.85 +0.7

Or see this region's nucleotide sequence