Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2318 and GFF2319 are separated by 45 nucleotides GFF2319 and GFF2320 are separated by 200 nucleotides GFF2320 and GFF2321 are separated by 25 nucleotides GFF2321 and GFF2322 are separated by 410 nucleotides
GFF2318 - LSU ribosomal protein L31p @ LSU ribosomal protein L31p, zinc-dependent, at 104,687 to 104,899
GFF2318
GFF2319 - FIG01046148: hypothetical protein, at 104,945 to 105,607
GFF2319
GFF2320 - Putative arylsulfate sulfotransferase (EC 2.8.2.22), at 105,808 to 107,457
GFF2320
GFF2321 - FIG01045707: hypothetical protein, at 107,483 to 107,644
GFF2321
GFF2322 - Methionine repressor MetJ, at 108,055 to 108,372
GFF2322
Position (kb)
105
106
107
108 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 104.952 kb on + strand at 104.987 kb on - strand at 105.035 kb on - strand, within GFF2319 at 105.074 kb on - strand, within GFF2319 at 105.096 kb on + strand, within GFF2319 at 105.347 kb on + strand, within GFF2319 at 105.446 kb on - strand, within GFF2319 at 105.498 kb on + strand, within GFF2319 at 105.498 kb on + strand, within GFF2319 at 105.499 kb on - strand, within GFF2319 at 105.568 kb on - strand at 105.568 kb on - strand at 105.569 kb on + strand at 105.570 kb on - strand at 105.613 kb on - strand at 105.634 kb on + strand at 105.743 kb on + strand at 105.775 kb on - strand at 105.881 kb on - strand at 105.952 kb on - strand at 106.057 kb on - strand, within GFF2320 at 106.288 kb on + strand, within GFF2320 at 106.297 kb on - strand, within GFF2320 at 106.366 kb on + strand, within GFF2320 at 106.425 kb on + strand, within GFF2320 at 106.426 kb on - strand, within GFF2320 at 106.426 kb on - strand, within GFF2320 at 106.426 kb on - strand, within GFF2320 at 106.426 kb on - strand, within GFF2320 at 106.426 kb on - strand, within GFF2320 at 106.690 kb on - strand, within GFF2320 at 106.704 kb on + strand, within GFF2320 at 106.716 kb on - strand, within GFF2320 at 106.716 kb on - strand, within GFF2320 at 106.760 kb on + strand, within GFF2320 at 106.884 kb on + strand, within GFF2320 at 106.937 kb on - strand, within GFF2320 at 106.938 kb on + strand, within GFF2320 at 106.971 kb on + strand, within GFF2320 at 107.086 kb on - strand, within GFF2320 at 107.086 kb on - strand, within GFF2320 at 107.102 kb on - strand, within GFF2320 at 107.145 kb on + strand, within GFF2320 at 107.213 kb on + strand, within GFF2320 at 107.284 kb on + strand, within GFF2320 at 107.284 kb on + strand, within GFF2320 at 107.392 kb on - strand at 107.392 kb on - strand at 107.404 kb on - strand at 107.406 kb on + strand at 107.461 kb on + strand at 107.461 kb on + strand at 107.462 kb on - strand at 108.324 kb on + strand, within GFF2322 at 108.382 kb on - strand at 108.400 kb on + strand at 108.444 kb on + strand at 108.449 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 104,952 + +1.9 104,987 - -0.9 105,035 - GFF2319 0.14 -0.1 105,074 - GFF2319 0.19 -0.0 105,096 + GFF2319 0.23 +0.1 105,347 + GFF2319 0.61 +1.0 105,446 - GFF2319 0.76 -0.1 105,498 + GFF2319 0.83 -0.2 105,498 + GFF2319 0.83 +0.9 105,499 - GFF2319 0.84 +0.7 105,568 - +0.8 105,568 - +1.2 105,569 + -0.2 105,570 - -0.5 105,613 - -1.4 105,634 + -0.4 105,743 + +1.0 105,775 - -1.2 105,881 - -1.7 105,952 - +0.3 106,057 - GFF2320 0.15 -3.3 106,288 + GFF2320 0.29 -4.8 106,297 - GFF2320 0.30 +2.0 106,366 + GFF2320 0.34 +0.4 106,425 + GFF2320 0.37 -1.2 106,426 - GFF2320 0.37 +0.5 106,426 - GFF2320 0.37 +0.9 106,426 - GFF2320 0.37 -1.6 106,426 - GFF2320 0.37 +0.9 106,426 - GFF2320 0.37 -3.8 106,690 - GFF2320 0.53 +0.6 106,704 + GFF2320 0.54 +0.5 106,716 - GFF2320 0.55 -2.6 106,716 - GFF2320 0.55 -0.4 106,760 + GFF2320 0.58 -0.8 106,884 + GFF2320 0.65 +2.7 106,937 - GFF2320 0.68 -0.3 106,938 + GFF2320 0.68 -0.7 106,971 + GFF2320 0.70 -0.3 107,086 - GFF2320 0.77 -0.1 107,086 - GFF2320 0.77 -1.3 107,102 - GFF2320 0.78 +1.6 107,145 + GFF2320 0.81 -1.5 107,213 + GFF2320 0.85 -0.1 107,284 + GFF2320 0.89 -0.7 107,284 + GFF2320 0.89 -1.7 107,392 - +0.6 107,392 - -1.7 107,404 - -1.9 107,406 + +0.3 107,461 + +0.7 107,461 + -1.0 107,462 - -0.4 108,324 + GFF2322 0.85 -1.2 108,382 - -3.1 108,400 + -1.6 108,444 + -1.0 108,449 + -3.7
Or see this region's nucleotide sequence