Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2226 and GFF2227 are separated by 1 nucleotides GFF2227 and GFF2228 overlap by 8 nucleotides GFF2228 and GFF2229 are separated by 51 nucleotides
GFF2226 - Hypothetical radical SAM family enzyme in interesting gene cluster, at 19,780 to 21,021
GFF2226
GFF2227 - FIG01124638: hypothetical protein, at 21,023 to 21,898
GFF2227
GFF2228 - FIG00638667: hypothetical protein, at 21,891 to 22,877
GFF2228
GFF2229 - FIG074102: hypothetical protein, at 22,929 to 23,777
GFF2229
Position (kb)
21
22
23 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 20.895 kb on - strand, within GFF2226 at 20.895 kb on - strand, within GFF2226 at 20.973 kb on + strand at 20.992 kb on - strand at 20.994 kb on - strand at 21.015 kb on + strand at 21.022 kb on - strand at 21.054 kb on + strand at 21.096 kb on + strand at 21.096 kb on + strand at 21.207 kb on + strand, within GFF2227 at 21.207 kb on + strand, within GFF2227 at 21.208 kb on - strand, within GFF2227 at 21.303 kb on + strand, within GFF2227 at 21.304 kb on - strand, within GFF2227 at 21.388 kb on + strand, within GFF2227 at 21.485 kb on - strand, within GFF2227 at 21.625 kb on + strand, within GFF2227 at 21.712 kb on - strand, within GFF2227 at 21.787 kb on + strand, within GFF2227 at 21.812 kb on - strand at 22.016 kb on + strand, within GFF2228 at 22.111 kb on - strand, within GFF2228 at 22.132 kb on + strand, within GFF2228 at 22.153 kb on - strand, within GFF2228 at 22.153 kb on - strand, within GFF2228 at 22.305 kb on - strand, within GFF2228 at 22.390 kb on + strand, within GFF2228 at 22.678 kb on + strand, within GFF2228 at 22.678 kb on + strand, within GFF2228 at 22.678 kb on + strand, within GFF2228 at 22.698 kb on + strand, within GFF2228 at 22.726 kb on - strand, within GFF2228 at 22.813 kb on - strand at 22.939 kb on + strand at 22.940 kb on - strand at 22.954 kb on + strand at 22.954 kb on + strand at 22.955 kb on - strand at 22.959 kb on - strand at 22.969 kb on + strand at 23.099 kb on + strand, within GFF2229 at 23.100 kb on - strand, within GFF2229 at 23.162 kb on + strand, within GFF2229 at 23.178 kb on - strand, within GFF2229 at 23.181 kb on - strand, within GFF2229 at 23.379 kb on + strand, within GFF2229 at 23.385 kb on - strand, within GFF2229 at 23.436 kb on + strand, within GFF2229 at 23.437 kb on - strand, within GFF2229 at 23.437 kb on - strand, within GFF2229 at 23.619 kb on - strand, within GFF2229 at 23.624 kb on + strand, within GFF2229 at 23.624 kb on + strand, within GFF2229 at 23.627 kb on + strand, within GFF2229 at 23.627 kb on + strand, within GFF2229 at 23.667 kb on - strand, within GFF2229 at 23.760 kb on - strand at 23.805 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 20,895 - GFF2226 0.90 -0.8 20,895 - GFF2226 0.90 +0.3 20,973 + +1.1 20,992 - -0.5 20,994 - -3.7 21,015 + -1.0 21,022 - +0.3 21,054 + +1.3 21,096 + +0.9 21,096 + +0.7 21,207 + GFF2227 0.21 -0.4 21,207 + GFF2227 0.21 -0.4 21,208 - GFF2227 0.21 -1.8 21,303 + GFF2227 0.32 +0.1 21,304 - GFF2227 0.32 +0.4 21,388 + GFF2227 0.42 -0.4 21,485 - GFF2227 0.53 -1.2 21,625 + GFF2227 0.69 -0.0 21,712 - GFF2227 0.79 +0.0 21,787 + GFF2227 0.87 -0.5 21,812 - +1.0 22,016 + GFF2228 0.13 +0.4 22,111 - GFF2228 0.22 +0.1 22,132 + GFF2228 0.24 -0.5 22,153 - GFF2228 0.27 +0.0 22,153 - GFF2228 0.27 +0.4 22,305 - GFF2228 0.42 +0.3 22,390 + GFF2228 0.51 -0.7 22,678 + GFF2228 0.80 -0.2 22,678 + GFF2228 0.80 -3.7 22,678 + GFF2228 0.80 +0.4 22,698 + GFF2228 0.82 +0.1 22,726 - GFF2228 0.85 -3.5 22,813 - -3.4 22,939 + +1.8 22,940 - -0.7 22,954 + +1.2 22,954 + -2.7 22,955 - +0.4 22,959 - +0.0 22,969 + +1.4 23,099 + GFF2229 0.20 -0.0 23,100 - GFF2229 0.20 -3.4 23,162 + GFF2229 0.27 -1.6 23,178 - GFF2229 0.29 -0.1 23,181 - GFF2229 0.30 -0.0 23,379 + GFF2229 0.53 -0.3 23,385 - GFF2229 0.54 -0.9 23,436 + GFF2229 0.60 +0.3 23,437 - GFF2229 0.60 +0.7 23,437 - GFF2229 0.60 +1.0 23,619 - GFF2229 0.81 +0.8 23,624 + GFF2229 0.82 +1.3 23,624 + GFF2229 0.82 +0.3 23,627 + GFF2229 0.82 -2.3 23,627 + GFF2229 0.82 +0.5 23,667 - GFF2229 0.87 +0.8 23,760 - -0.4 23,805 - +0.6
Or see this region's nucleotide sequence