Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2186 and GFF2187 are separated by 3 nucleotides GFF2187 and GFF2188 are separated by 573 nucleotides GFF2188 and GFF2189 are separated by 184 nucleotides
GFF2186 - N-succinyl-L,L-diaminopimelate desuccinylase (EC 3.5.1.18), at 32,563 to 33,690
GFF2186
GFF2187 - FIG138056: a glutathione-dependent thiol reductase, at 33,694 to 34,050
GFF2187
GFF2188 - RND efflux system, inner membrane transporter CmeB, at 34,624 to 37,737
GFF2188
GFF2189 - Nitrate/nitrite sensor protein (EC 2.7.3.-), at 37,922 to 39,622
GFF2189
Position (kb) 34
35
36
37
38 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 33.720 kb on - strand at 33.921 kb on + strand, within GFF2187 at 33.940 kb on + strand, within GFF2187 at 34.322 kb on - strand at 34.416 kb on + strand at 34.428 kb on + strand at 34.437 kb on + strand at 34.789 kb on + strand at 34.967 kb on + strand, within GFF2188 at 35.069 kb on + strand, within GFF2188 at 35.288 kb on + strand, within GFF2188 at 35.288 kb on + strand, within GFF2188 at 35.376 kb on - strand, within GFF2188 at 35.376 kb on - strand, within GFF2188 at 35.404 kb on - strand, within GFF2188 at 35.408 kb on + strand, within GFF2188 at 35.429 kb on + strand, within GFF2188 at 35.429 kb on + strand, within GFF2188 at 35.430 kb on - strand, within GFF2188 at 35.430 kb on - strand, within GFF2188 at 35.430 kb on - strand, within GFF2188 at 35.430 kb on - strand, within GFF2188 at 35.458 kb on - strand, within GFF2188 at 35.471 kb on + strand, within GFF2188 at 36.021 kb on + strand, within GFF2188 at 36.021 kb on + strand, within GFF2188 at 36.108 kb on - strand, within GFF2188 at 36.247 kb on + strand, within GFF2188 at 36.248 kb on - strand, within GFF2188 at 36.251 kb on - strand, within GFF2188 at 36.339 kb on - strand, within GFF2188 at 36.524 kb on - strand, within GFF2188 at 36.571 kb on - strand, within GFF2188 at 36.588 kb on - strand, within GFF2188 at 36.614 kb on - strand, within GFF2188 at 36.671 kb on + strand, within GFF2188 at 36.672 kb on - strand, within GFF2188 at 36.672 kb on - strand, within GFF2188 at 36.770 kb on + strand, within GFF2188 at 36.915 kb on - strand, within GFF2188 at 37.035 kb on + strand, within GFF2188 at 37.194 kb on - strand, within GFF2188 at 37.194 kb on - strand, within GFF2188 at 37.194 kb on - strand, within GFF2188 at 37.194 kb on - strand, within GFF2188 at 37.205 kb on + strand, within GFF2188 at 37.294 kb on - strand, within GFF2188 at 37.508 kb on + strand at 37.509 kb on - strand at 37.509 kb on - strand at 37.605 kb on + strand at 37.610 kb on - strand at 37.634 kb on + strand at 37.763 kb on + strand at 37.764 kb on - strand at 37.833 kb on + strand at 37.838 kb on + strand at 37.923 kb on + strand at 37.966 kb on + strand at 38.042 kb on + strand at 38.043 kb on - strand at 38.108 kb on - strand, within GFF2189 at 38.488 kb on - strand, within GFF2189 at 38.496 kb on - strand, within GFF2189 at 38.589 kb on + strand, within GFF2189 at 38.644 kb on + strand, within GFF2189
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 33,720 - +0.4 33,921 + GFF2187 0.64 +0.2 33,940 + GFF2187 0.69 +1.8 34,322 - -0.2 34,416 + +0.2 34,428 + +0.2 34,437 + -0.4 34,789 + +0.7 34,967 + GFF2188 0.11 +1.2 35,069 + GFF2188 0.14 -0.5 35,288 + GFF2188 0.21 -0.2 35,288 + GFF2188 0.21 +0.9 35,376 - GFF2188 0.24 -3.6 35,376 - GFF2188 0.24 +1.9 35,404 - GFF2188 0.25 -0.3 35,408 + GFF2188 0.25 -1.0 35,429 + GFF2188 0.26 -0.0 35,429 + GFF2188 0.26 -4.1 35,430 - GFF2188 0.26 +1.1 35,430 - GFF2188 0.26 -0.8 35,430 - GFF2188 0.26 +0.5 35,430 - GFF2188 0.26 +0.2 35,458 - GFF2188 0.27 +0.3 35,471 + GFF2188 0.27 -0.4 36,021 + GFF2188 0.45 +0.1 36,021 + GFF2188 0.45 -0.2 36,108 - GFF2188 0.48 +0.4 36,247 + GFF2188 0.52 -0.4 36,248 - GFF2188 0.52 +0.7 36,251 - GFF2188 0.52 +0.5 36,339 - GFF2188 0.55 -0.3 36,524 - GFF2188 0.61 -4.3 36,571 - GFF2188 0.63 +0.1 36,588 - GFF2188 0.63 +0.4 36,614 - GFF2188 0.64 -2.2 36,671 + GFF2188 0.66 -1.4 36,672 - GFF2188 0.66 +0.8 36,672 - GFF2188 0.66 -0.3 36,770 + GFF2188 0.69 -1.0 36,915 - GFF2188 0.74 +0.2 37,035 + GFF2188 0.77 +0.2 37,194 - GFF2188 0.83 +0.4 37,194 - GFF2188 0.83 -0.0 37,194 - GFF2188 0.83 +0.1 37,194 - GFF2188 0.83 -0.4 37,205 + GFF2188 0.83 +0.9 37,294 - GFF2188 0.86 -0.1 37,508 + -3.4 37,509 - -1.8 37,509 - -0.3 37,605 + +0.6 37,610 - -0.2 37,634 + +0.8 37,763 + +0.3 37,764 - -0.3 37,833 + +0.3 37,838 + -0.3 37,923 + +0.4 37,966 + +0.6 38,042 + +0.8 38,043 - -1.5 38,108 - GFF2189 0.11 -0.1 38,488 - GFF2189 0.33 -3.4 38,496 - GFF2189 0.34 -0.0 38,589 + GFF2189 0.39 +2.0 38,644 + GFF2189 0.42 -1.5
Or see this region's nucleotide sequence