Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2056 and GFF2057 overlap by 11 nucleotides GFF2057 and GFF2058 overlap by 1 nucleotides GFF2058 and GFF2059 overlap by 1 nucleotides
GFF2056 - Sulfate and thiosulfate import ATP-binding protein CysA (EC 3.6.3.25), at 20,649 to 21,746
GFF2056
GFF2057 - Sulfate transport system permease protein CysW, at 21,736 to 22,611
GFF2057
GFF2058 - Sulfate transport system permease protein CysT, at 22,611 to 23,444
GFF2058
GFF2059 - Sulfate and thiosulfate binding protein CysP, at 23,444 to 24,460
GFF2059
Position (kb) 22
23
24 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 21.832 kb on + strand, within GFF2057 at 21.875 kb on + strand, within GFF2057 at 21.901 kb on + strand, within GFF2057 at 22.003 kb on + strand, within GFF2057 at 22.004 kb on - strand, within GFF2057 at 22.066 kb on + strand, within GFF2057 at 22.310 kb on - strand, within GFF2057 at 22.310 kb on - strand, within GFF2057 at 22.620 kb on + strand at 22.621 kb on - strand at 23.031 kb on - strand, within GFF2058 at 23.071 kb on + strand, within GFF2058 at 23.072 kb on - strand, within GFF2058 at 23.239 kb on - strand, within GFF2058 at 23.361 kb on + strand at 23.361 kb on + strand at 23.405 kb on + strand at 23.449 kb on + strand at 23.450 kb on - strand at 23.566 kb on + strand, within GFF2059 at 23.567 kb on - strand, within GFF2059 at 23.610 kb on + strand, within GFF2059 at 23.610 kb on + strand, within GFF2059 at 23.611 kb on - strand, within GFF2059 at 23.779 kb on - strand, within GFF2059 at 23.779 kb on - strand, within GFF2059 at 23.815 kb on + strand, within GFF2059 at 23.976 kb on + strand, within GFF2059 at 24.201 kb on + strand, within GFF2059 at 24.274 kb on + strand, within GFF2059 at 24.275 kb on - strand, within GFF2059 at 24.364 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 21,832 + GFF2057 0.11 +2.0 21,875 + GFF2057 0.16 -2.4 21,901 + GFF2057 0.19 +0.1 22,003 + GFF2057 0.30 -1.2 22,004 - GFF2057 0.31 +1.0 22,066 + GFF2057 0.38 -1.2 22,310 - GFF2057 0.66 -0.0 22,310 - GFF2057 0.66 -1.3 22,620 + +1.8 22,621 - -1.4 23,031 - GFF2058 0.50 -0.4 23,071 + GFF2058 0.55 +0.9 23,072 - GFF2058 0.55 +0.3 23,239 - GFF2058 0.75 -1.7 23,361 + -3.3 23,361 + -0.5 23,405 + -3.4 23,449 + -0.6 23,450 - -0.2 23,566 + GFF2059 0.12 +0.1 23,567 - GFF2059 0.12 -0.2 23,610 + GFF2059 0.16 +0.2 23,610 + GFF2059 0.16 +0.7 23,611 - GFF2059 0.16 +0.5 23,779 - GFF2059 0.33 +0.0 23,779 - GFF2059 0.33 +0.2 23,815 + GFF2059 0.36 -0.4 23,976 + GFF2059 0.52 -4.1 24,201 + GFF2059 0.74 -1.8 24,274 + GFF2059 0.82 -2.4 24,275 - GFF2059 0.82 +0.6 24,364 - -0.2
Or see this region's nucleotide sequence