Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1826

Experiment: 6H2_phage 10 MOI; agar plate assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1825 and GFF1826 are separated by 75 nucleotidesGFF1826 and GFF1827 are separated by 2 nucleotides GFF1825 - Type I secretion system ATPase, LssB family LapB, at 7,155 to 8,798 GFF1825 GFF1826 - Alkylated DNA repair protein AlkB, at 8,874 to 9,524 GFF1826 GFF1827 - ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63), at 9,527 to 10,585 GFF1827 Position (kb) 8 9 10Strain fitness (log2 ratio) -3 -2 -1 0 1at 7.997 kb on - strand, within GFF1825at 8.138 kb on - strand, within GFF1825at 8.243 kb on - strand, within GFF1825at 8.243 kb on - strand, within GFF1825at 8.249 kb on + strand, within GFF1825at 8.250 kb on - strand, within GFF1825at 8.444 kb on + strand, within GFF1825at 8.445 kb on - strand, within GFF1825at 8.445 kb on - strand, within GFF1825at 8.447 kb on + strand, within GFF1825at 8.448 kb on - strand, within GFF1825at 8.802 kb on + strandat 8.802 kb on + strandat 8.860 kb on - strandat 9.018 kb on + strand, within GFF1826at 9.019 kb on - strand, within GFF1826at 9.228 kb on + strand, within GFF1826at 9.229 kb on - strand, within GFF1826at 9.229 kb on - strand, within GFF1826at 9.266 kb on + strand, within GFF1826at 9.267 kb on - strand, within GFF1826at 9.292 kb on + strand, within GFF1826at 9.292 kb on + strand, within GFF1826at 9.427 kb on - strand, within GFF1826at 9.529 kb on - strandat 9.557 kb on + strandat 9.912 kb on - strand, within GFF1827at 10.095 kb on + strand, within GFF1827at 10.095 kb on + strand, within GFF1827at 10.096 kb on - strand, within GFF1827at 10.099 kb on + strand, within GFF1827at 10.100 kb on - strand, within GFF1827at 10.100 kb on - strand, within GFF1827at 10.100 kb on - strand, within GFF1827at 10.100 kb on - strand, within GFF1827at 10.333 kb on + strand, within GFF1827

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Per-strain Table

Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay
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7,997 - GFF1825 0.51 -1.5
8,138 - GFF1825 0.60 +1.4
8,243 - GFF1825 0.66 -0.1
8,243 - GFF1825 0.66 -0.4
8,249 + GFF1825 0.67 -0.6
8,250 - GFF1825 0.67 -0.2
8,444 + GFF1825 0.78 +0.1
8,445 - GFF1825 0.78 -1.2
8,445 - GFF1825 0.78 -1.5
8,447 + GFF1825 0.79 -0.5
8,448 - GFF1825 0.79 -1.0
8,802 + +0.1
8,802 + -1.7
8,860 - -0.7
9,018 + GFF1826 0.22 +0.6
9,019 - GFF1826 0.22 +0.2
9,228 + GFF1826 0.54 +1.2
9,229 - GFF1826 0.55 +0.4
9,229 - GFF1826 0.55 +0.3
9,266 + GFF1826 0.60 +0.7
9,267 - GFF1826 0.60 -3.2
9,292 + GFF1826 0.64 +0.8
9,292 + GFF1826 0.64 +0.3
9,427 - GFF1826 0.85 +1.0
9,529 - +0.3
9,557 + -0.4
9,912 - GFF1827 0.36 +1.7
10,095 + GFF1827 0.54 -0.1
10,095 + GFF1827 0.54 -0.7
10,096 - GFF1827 0.54 +0.0
10,099 + GFF1827 0.54 -2.3
10,100 - GFF1827 0.54 +0.2
10,100 - GFF1827 0.54 +0.6
10,100 - GFF1827 0.54 +0.2
10,100 - GFF1827 0.54 -2.6
10,333 + GFF1827 0.76 +1.7

Or see this region's nucleotide sequence